Efficient differentiation of vascular endothelial cells from dermal-derived mesenchymal stem cells induced by endothelial cell lines conditioned medium

Zhou, Ling; Niu, Xuping; Liang, Jiannan; Li, Junqin; Li, Jiao; Cheng, Yueai; Meng, Yanfeng; Wang, Qiang; Yang, Xiaoli; Wang, Gang; Shi, Yu; Dang, Erle; Zhang, Kaiming

doi:10.1101/271148

Cited by 4 publications

(5 citation statements)

References 22 publications

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“…The VEGF release may accelerate cell migration (Mohsen, Hatef, Hamid, Ali, & Ghasem, ; Torio‐Padron et al, ). However, it seems that keeping VEGF in the scaffold supports vascularization because it not only enhances the proliferation of cells (Carpenter et al, ; Zhou et al, ), but also regulates the interaction between stem cells and ECs (such as directional differentiation of stem cells induced by ECs and pore size in the scaffold) and promotes angiogenesis (Adibfar et al, ; Cho, Moon, Park, Lee, & Seo, ; Zhou et al, ). Thus, in the present study, we designed two kinds of nanoparticles to load SDF‐1 and VEGF, respectively.…”

Section: Discussionmentioning

confidence: 99%

An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

Zhao

et al. 2019

J Biomedical Materials Res

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Controlled release of functional factors contributes to target migration of therapeutic cells and plays a crucial role in the in situ vascularization of tissue repair and regeneration. A biomedical application requires the selective release of multiple factors which will guide the synergy of the cells. Here, we developed an injectable system based on a temperature-responsive hydrogel and stromal cell-derived factor-1 (SDF-1)/vascular endothelial growth factor (VEGF) loaded into two types of nanoparticles to induce migration and rapid proliferation of mesenchymal stem cells (MSCs) and endothelial cells (ECs) via selective SDF-1/VEGF release. Series of in vitro and in vivo experiments demonstrate that our composited system can accurately guide MSCs and ECs for vascularization. In addition, the properties of the nanoparticles and hydrogel, including micro/nanoscales, characteristic of charge, and biocompatibility, played crucial roles for the selective release and cells behavior (target migration and rapid proliferation).

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Section: Discussionmentioning

confidence: 99%

An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

Zhao

et al. 2019

J Biomedical Materials Res

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“…To further verify that whether the effect of miR‐103 on the HFD‐induced atherosclerotic lesions in ApoE −/− mice was correlated with the PECAM‐1 + endothelial cells. First, PECAM‐1 + endothelial cells were sorted out using flow cytometry (Figure a; Zhou et al, ). RT‐qPCR (Figure b) revealed that miR‐103 expression in PECAM‐1 + endothelial cells of ApoE −/− mice fed a HFD were higher than that in PECAM‐1 + endothelial cells of ApoE −/− mice fed an ND.…”

Section: Resultsmentioning

confidence: 99%

Inhibition of microRNA‐103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN‐mediated MAPK signaling

Jiang

Qiao

Wang

et al. 2019

Journal Cellular Physiology

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Atherosclerosis (AS), a chronic disorder of large arteries, is the underlying pathological process of heart disease and stroke. Former researchers have found that microRNAs (miRs) are involved in the several key processes of AS. Apolipoprotein E knockout (ApoE −/− ) mice fed a high-fat-diet (HFD) to establish AS model. The expression of miR-103 was characterized in the mice model. The effects of miR-103 on inflammation and endoplasmic reticulum stress (ERS) were analyzed when the expression of miR-103 was inhibited in ApoE −/− mice fed an HFD and human aortic endothelial cells (HAECs) exposed to oxidized low-density lipoprotein (ox-LDL). The relationship between miR-103 and phosphatase and tensin homolog (PTEN) was identified by luciferase activity detection and real-time quantitative polymerase chain reaction (RT-qPCR). Gain-and loss-function approaches were further applied for investigating the regulatory effects of miR-103 and PTEN on ERS. Role of MAPK signaling was then analyzed using PD98059 to block this pathway. miR-103 was highly expressed in the ApoEApoE −/− mice fed an HFD. Downregulation of miR-103 suppressed inflammation and ERS in endothelial cells isolated from ApoE −/− mice fed a HFD and ox-LDL-exposed HAECs. In addition, miR-103 can target PTEN and downregulate its expression. Overexpression of PTEN reversed the miR-103-induced activation of MAPK signaling. Moreover, PTEN upregulation or MAPK signaling inhibition ease miR-103-induced inflammation and ERS in vivo and in vitro. Thus, miR-103 depletion restrains the progression of AS through blocking PTEN-mediated MAPK signaling.

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“…However, there is a challenge to get effective induction of blood vessels into engineered graft in tissue engineering. The major obstacle is to obtain an abundant source of e cient autologous endothelial cells [25]. [29].…”

Section: Discussionmentioning

confidence: 99%

Biomimetic vascularized adipose-derived mesenchymal stem cells bone-periosteum graft enhances angiogenesis and osteogenesis in a rabbit spine fusion model

Fu¹,

Chen²,

Wang³

et al. 2021

Preprint

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BackgroundSeveral artificial bone grafts have been developed for bone reconstruction but fail to achieve anticipated osteogenesis due to their insufficient neovascularization capacity. Besides, periosteum plays an essential role in the neovascularization process in bone formation and healing. The aim of this study was to develop a cell-based biomimetic vascularized bone-periosteum construct (VBPC) to provide better neovascularization for osteogenesis and bone regeneration.MethodsTwenty-four male New Zealand white rabbits were divided into four groups according to the experimental materials. We first cultured adipose-derived mesenchymal stem cells (AMSCs) and seeded them evenly in the collagen/chitosan sheet to form an AMSCs-sheet-engineered periosteum. Simultaneously, the AMSCs were seeded onto alginate scaffolds and were cultured to differentiate to endothelial-like cells to form vascularized bone constructs (VBC). The success of endothelial differentiation was confirmed by real-time polymerase chain reaction and immunofluorescence staining analysis. The AMSCs-sheet-engineered periosteum was wrapped onto VBC to create biomimetic VBPC, which was then implanted in bilateral L4-5 intertransverse space of rabbit. The acellular alginate-sheet construct, VBC, and non-vascularized AMSCs-alginate-periosteum construct were used as controls. At 12 weeks after implantation, the bone-forming capacities of the constructs were determined by computed tomography, biomechanical testing, histology, and immunohistochemistry staining analyses.ResultsTwelve weeks after implantation, the VBPC group significantly increased new bone formation volume than the control groups. Biomechanical testing demonstrated a higher torque strength in the VBPC group, and suggested that the cell sheet played a critical role for mechanical support. Notably, the hematoxylin and eosin, Masson’s trichrome, and immunohistochemistry stained histologic results revealed that the VBPC group promoted the formation of blood vessels and new bones in the L4-5 intertransverse fusion areas.ConclusionsThe tissue-engineered biomimetic VBPC showed great capability in promoting angiogenesis and osteogenesis in vivo. The VBPC may overcome the deficits of traditional bone grafts. These findings suggest a novel approach to improve the timely formation of blood vessels from bone substitutes and provide an ideal source for bone regeneration.

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Efficient differentiation of vascular endothelial cells from dermal-derived mesenchymal stem cells induced by endothelial cell lines conditioned medium

Cited by 4 publications

References 22 publications

An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

An injectable scaffold based on temperature‐responsive hydrogel and factor‐loaded nanoparticles for application in vascularization in tissue engineering

Inhibition of microRNA‐103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN‐mediated MAPK signaling

Biomimetic vascularized adipose-derived mesenchymal stem cells bone-periosteum graft enhances angiogenesis and osteogenesis in a rabbit spine fusion model

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