Multifunctional Biomimetic Nanofibrous Scaffold Loaded with Asiaticoside for Rapid Diabetic Wound Healing

Abstract

Diabetes mellitus is a chronic disease with a high mortality rate and many complications. A non-healing diabetic foot ulcer (DFU) is one the most serious complications, leading to lower-extremity amputation in 15% of diabetic patients. Nanofibers are emerging as versatile wound dressing due to their unique wound healing properties, such as a high surface area to volume ratio, porosity, and ability to maintain a moist wound environment capable of delivering sustained drug release and oxygen supply to a wound. The present study was aimed to prepare and evaluate a polyvinyl alcohol (PVA)–sodium alginate (SA)–silk fibroin (SF)-based multifunctional nanofibrous scaffold loaded with asiaticoside (AT) in diabetic rats. The SEM findings showed that fibers’ diameters ranged from 100–200 nm, and tensile strengths ranged from 12.41–16.80 MPa. The crosslinked nanofibers were sustained AT over an extended period. The MTT and scratch assay on HaCat cells confirmed low cytotoxicity and significant cell migration, respectively. Antimicrobial tests revealed an excellent anti-microbial efficacy against P. aeruginosa and S. aureus bacteria. In-vivo study demonstrated better wound healing efficacy in diabetic rats. In addition, the histopathological studies showed its ability to restore the normal structure of the skin. The present study concluded that developed multifunctional nanofibers have a great potential for diabetic wound healing applications.

Asiaticoside delays senescence and attenuate generation of ROS in UV‑exposure cells through regulates TGF‑β1/Smad pathway

“…Not only capacity of anti-aging in UV damaged cells as reported in the present study, but also capability against retinal degradation and ameliorate inflammation through Nrf2/HO-1 pathway and TLR4/NF-κB pathway, respectively ( 57 , 58 ). Furthermore, asiaticoside-contained foam dressing, nano-composite and nanofibrous scaffold have been developed for traumatic treatment, showing great potential of asiaticoside in wound healing ( 14 , 59 , 60 ). By taking advantage of nanocarriers, novel usages of asiaticoside could be expanded and more applications related to asiaticoside might be developed.…”

Electrospun Biomimetic Multifunctional Nanofibers Loaded with Ferulic Acid for Enhanced Antimicrobial and Wound-Healing Activities in STZ-Induced Diabetic Rats

“…Diabetic wound control (toxic control) was used in group I, placebo control was used in group II, and FA-SS-PCL-NF and FA-SS-CA-NF treatments were used in groups III and IV, respectively. The differences in the wound areas were observed at intervals of 0, 5, 9, and 14 days after treatment [ 34 , 35 , 36 , 37 ].…”

Polysaccharide Electrospun Nanofibers for Wound Healing Applications

“… 93 The main causes of delayed wound healing are chronic inflammation, growth factor secretion disorder, microbial infection and destruction of angiogenesis. 94 , 95 In addition, reduced proliferation, migration, and relocation of fibroblasts severely impede the wound healing process. Therefore, the development of wound dressings with specific functions such as antibacterial, anti-inflammatory, promotion of cell proliferation and migration, and promotion of angiogenesis can correct the disrupted stages of wound healing and guide the wound to follow an orderly healing procedure.…”