Electrospun Biomimetic Multifunctional Nanofibers Loaded with Ferulic Acid for Enhanced Antimicrobial and Wound-Healing Activities in STZ-Induced Diabetic Rats

Anand, Sneha; Pandey, Prashant; Begum, M. Yasmin; Chidambaram, Kumarappan; Arya, Dilip Kumar; Gupta, Ravi; Sankhwar, Ruchi; Jaiswal, Shweta; Thakur, S.S.; Rajinikanth, Paruvathanahalli Siddalingam

doi:10.3390/ph15030302

Cited by 35 publications

(18 citation statements)

References 48 publications

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“…To a certain extent, the fields of pharmaceutics and drug delivery rely heavily on the inputs of materials sciences (new excipients) and engineering (new techniques) [ 8 , 9 , 10 , 11 , 12 , 13 ]. Thus, during the past half a century, on the one hand, more and more types of materials (including natural polymers, synthesis polymers, phospholipid, surfactants, and even inorganic materials) have been tried as new drug carriers for adjusting the drug release performances [ 14 , 15 , 16 , 17 , 18 ]. Among them, polymeric excipients are the main stream [ 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Films Prepared from a Combination of Electrospinning and Casting for Offering a Dual-Phase Drug Release

et al. 2022

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One of the most important trends in developments in electrospinning is to combine itself with traditional materials production and transformation methods to take advantage of the unique properties of nanofibers. In this research, the single-fluid blending electrospinning process was combined with the casting film method to fabricate a medicated double-layer hybrid to provide a dual-phase drug controlled release profile, with ibuprofen (IBU) as a common model of a poorly water-soluble drug and ethyl cellulose (EC) and polyvinylpyrrolidone (PVP) K60 as the polymeric excipients. Electrospun medicated IBU-PVP nanofibers (F7), casting IBU-EC films (F8) and the double-layer hybrid films (DHFs, F9) with one layer of electrospun nanofibers containing IBU and PVP and the other layer of casting films containing IBU, EC and PVP, were prepared successfully. The SEM assessments demonstrated that F7 were in linear morphologies without beads or spindles, F8 were solid films, and F9 were composed of one porous fibrous layer and one solid layer. XRD and FTIR results verified that both EC and PVP were compatible with IBU. In vitro dissolution tests indicated that F7 were able to provide a pulsatile IBU release, F8 offered a typical drug sustained release, whereas F9 were able to exhibit a dual-phase controlled release with 40.3 ± 5.1% in the first phase for a pulsatile manner and the residues were released in an extended manner in the second phase. The DHFs from a combination of electrospinning and the casting method pave a new way for developing novel functional materials.

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Section: Introductionmentioning

confidence: 99%

Hybrid Films Prepared from a Combination of Electrospinning and Casting for Offering a Dual-Phase Drug Release

et al. 2022

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“…The formulation parameters, like concentration of polymer, voltage (kV), the distance between the tip and collector and the flow rate, were optimized and selected based on the earlier published literature. 3 , 24 SEM image confirmed the surface topography, diameter and uniformity of developed nanofibers. The average diameter of CS-PVA-ZnO-CUR nanofiber was found to be 274.16 ± 91.43 nm as depicted in Figure 1A and their corresponding histogram in Figure 1B , whereas the average diameter of CS-PVA-ZnO-CUR (CL) nanofiber was found 297.38 ± 69.86 nm, Figure 1C and their histogram Figure 1D .…”

Section: Resultsmentioning

confidence: 58%

“…The antimicrobial activity of nanofibers was determined using the disc diffusion method of agar well towards Gram-ve, Pseudomonas aeruginosa (ATCC 27853, Boston, MA, USA) and Gram+ve bacteria, Staphylococcus aureus (ATCC 25923, Seattle, WA, USA). 24 For 24 hrs, P. aeruginosa was cultured using Luria Bertani broth (LB) and S. aureus using Tryptone soy broth (TSB) (HiMedia LQ508) aqueous media in an orbital shaker at 37 °C and 200 rpm. The bacterial cultures were smeared in triplicate on MHA agar plate (Mueller Hinton) (HiMedia; M173).…”

Section: Characterizationmentioning

confidence: 99%

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ECM Mimicking Biodegradable Nanofibrous Scaffold Enriched with Curcumin/ZnO to Accelerate Diabetic Wound Healing via Multifunctional Bioactivity

Yadav

Arya

Pandey

et al. 2022

IJN

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Introduction Foot ulceration is one of the most severe and debilitating complications of diabetes, which leads to the cause of non-traumatic lower-extremity amputation in 15–24% of affected individuals. The healing of diabetic foot (DF) is a significant therapeutic problem due to complications from the multifactorial healing process. Electrospun nanofibrous scaffold loaded with various wound dressing materials has excellent wound healing properties due to its multifunctional action. Purpose This work aimed to develop and characterize chitosan (CS)-polyvinyl alcohol (PVA) blended electrospun multifunctional nanofiber loaded with curcumin (CUR) and zinc oxide (ZnO) to accelerate diabetic wound healing in STZ-induced diabetic rats. Results In-vitro characterization results revealed that nanofiber was fabricated successfully using the electrospinning technique. SEM results confirmed the smooth surface with web-like fiber nanostructure diameter ranging from 200 – 250 nm. An in-vitro release study confirmed the sustained release of CUR and ZnO for a prolonged time. In-vitro cell-line studies demonstrated significantly low cytotoxicity of nanofiber in HaCaT cells. Anti-bacterial studies demonstrated good anti-bacterial and anti-biofilm activities of nanofiber. In-vivo animal studies demonstrated an excellent wound-healing efficiency of the nanofibers in STZ–induced diabetic rats. Furthermore, the ELISA assay revealed that the optimized nanofiber membrane terminated the inflammatory phases successfully by downregulating the pro-inflammatory cytokines (TNF-α, MMP-2, and MMP-9) in wound healing. In-vitro and in-vivo studies conclude that the developed nanofiber loaded with bioactive material can promote diabetic wound healing efficiently via multifunction action such as the sustained release of bioactive molecules for a prolonged time of duration, proving anti-bacterial/anti-biofilm properties and acceleration of cell migration and proliferation process during the wound healing. Discussion CUR-ZnO electrospun nanofibers could be a promising drug delivery platform with the potential to be scaled up to treat diabetic foot ulcers effectively.

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“…Biocomposite films with 8 mm diameter were positioned in the well. To determine the inhibition zone, Petri dishes were incubated at 37 ± 1 °C for 24 hours [32,33].…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Preparation and Antimicrobial Activity of Corn Cob and Coir Reinforced Biodegradable Starch Biocomposite Films for Food Packaging Application

Nanda

Singh

2022

AFSJ

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Finding a bio-composite film to replace petroleum-based synthetic plastic has received considerable attention in recent years. In this research, biocomposite films were effectively prepared using corn cob powder and coconut coir as reinforcing materials in various concentrations using solution casting and annealing techniques. Corn starch was used as a co-biopolymer, glycerol (3% v/v) and acetic acid (5% v/v) as plasticizers were added as well. Biodegradability, mechanical, physical, FTIR, SEM, XRD, tensile and antibacterial tests were performed on the biocomposite samples. The biodegradability test showed that the samples were biodegradable and that the rate of biodegradation improved with time, reaching 87.37 % in just ten days. The SEM test was used to investigate the molecular structure of the biocomposite films, and the findings demonstrated that the raw ingredients had been well mixed, resulting in a smooth surface. The antibacterial test was conducted against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative) bacteria. This study examines the influence of fiber reinforcement on the mechanical properties of biocomposites based on starch. Various natural fibers may boost the strength of biocomposites, as shown by the findings. According to the results, maize starch, corn cobs, and coconut coir are all suitable for food packaging purposes.

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Electrospun Biomimetic Multifunctional Nanofibers Loaded with Ferulic Acid for Enhanced Antimicrobial and Wound-Healing Activities in STZ-Induced Diabetic Rats

Cited by 35 publications

References 48 publications

Hybrid Films Prepared from a Combination of Electrospinning and Casting for Offering a Dual-Phase Drug Release

Hybrid Films Prepared from a Combination of Electrospinning and Casting for Offering a Dual-Phase Drug Release

ECM Mimicking Biodegradable Nanofibrous Scaffold Enriched with Curcumin/ZnO to Accelerate Diabetic Wound Healing via Multifunctional Bioactivity

Preparation and Antimicrobial Activity of Corn Cob and Coir Reinforced Biodegradable Starch Biocomposite Films for Food Packaging Application

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