Morphogenesis and tissue cultures of three citrus species

Duran-Vila, N.; Ortega, Victoria; Navarro, Luís

doi:10.1007/bf00036520

Cited by 63 publications

(40 citation statements)

References 9 publications

(6 reference statements)

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“…In Citrus, the morphogenic response of in vitro cultures depends on genotype, explant type and orientation, composition of culture medium, and conditions of incubation (Durán-Vila et al, 1989;Ghorbel et al, 1998;García-Luis et al, 1999;Bordon et al, 2000;Costa et al, 2004). Epicotyl segments from in vitro germinated seedlings have a higher morphogenic ability than other tissues used as explant source.…”

Section: Parâmetros De Cultura De Tecidos Em Cultivares De Laranjeiramentioning

confidence: 99%

Tissue culture parameters in sweet orange cultivars

Mendes

Cidade

Manzoli

et al. 2008

Pesq. agropec. bras.

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-The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specifi c. Epicotyl explants from 35-day-old seedlings produced signifi cantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was signifi cantly higher in medium with NAA and IBA than with NAA alone.

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Section: Parâmetros De Cultura De Tecidos Em Cultivares De Laranjeiramentioning

confidence: 99%

Tissue culture parameters in sweet orange cultivars

Mendes

Cidade

Manzoli

et al. 2008

Pesq. agropec. bras.

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“…Tissue culture and micropropagation protocols have been described for a number of Citrus spp. using a wide range of explant sources (Grinblat 1972;Barlass and Skene 1982;Duran-Vila et al 1989;Beloualy 1991;Carimi et al 1995;Altaf and Ahmad 1997;Normah et al 1997;Al-Khayri and Al-Bahrany 2001;Khawale and Singh 2005;Ali and Mirza 2006;Altaf et al 2008;Altaf et al 2009a, b;Khan et al 2009;Laskar et al 2009;Sharma et al 2009;Pe'rez-Tornero et al 2010;Singh and Rajam 2009;. However, a little work has been carried out on the tissue culture of C. jambhiri (Raman et al 1992;Altaf and Ahmad 1997;Khawale and Singh 2005;Ali and Mirza 2006;Altaf et al 2008;Sharma et al 2009;Savita et al 2010).…”

Section: Introductionmentioning

confidence: 99%

An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

Savita

Singh

Virk

et al. 2011

Physiol Mol Biol Plants

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Citrus jambhiri Lush. (family Rutaceae), commonly known as 'rough lemon', is one of the favourite rootstocks for lemons, oranges, mandarins, grape fruits and kinnows in Punjab. The present investigation deals with development of an efficient miropropagation protocol for Citrus jambhiri Lush. using cotyledons as explant. Maximum callus induction (91.66 %) was observed on MS medium supplemented with 2,4-D (2 mg/L) in combination with ME (500 mg/L). Green healthy calli were cut into small pieces and cultured on MS medium for regeneration. Maximum shoot regeneration (87.50 %) was observed with BA (3 mg/L). Effect of increasing age of callus was also studied which showed that callus retained regeneration capacity (58.33 %) even after 420 days of culture. Regenerated shoots were separated out and cultured on rooting medium. Maximum rooting response (91.67 %) was observed on half strength MS medium supplemented with NAA (0.5 mg/L). After hardening and acclimatization the plantlet were transferred to the field and showed 67 % survival.

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“…and mandarin (C. reticulata Blanco cv. Monica) using 7.5 mg dm The maximum induction of adventitious buds in 'Pineapple' orange (Citrus sinensis) and citron (Citrus medica) explants was obtained on MS medium with 3.0 mg dm -3 BAP, while for lime (Citrus aurantifolia) 1.0 mg dm -3 BAP showed better results in explants obtained from internodal segments of greenhouse cultivated plants (Duran-Vila & Navarro, 1989).…”

Section: In Vitro Induction and Regeneration Of Adventitious Buds Andmentioning

confidence: 97%

Sour orange bud regeneration and in vitro plant development related to culture medium composition and explant type

et al. 2010

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-In order to evaluate the formation of adventitious buds and in vitro regeneration of sour orange plants (Citrus aurantium L.) two organogenesis-inducing experiments were conducted. In the fi rst experiment, the induction and in vitro regeneration of adventitious buds were tested on epicotyl and internodal segments under the infl uence of BAP or KIN associated with NAA. The second experiment evaluated the in vitro regeneration of sour orange plants related to different explant types (epicotyls segments, internodal segments of in vitro germinated plantlets and internodal segments of greenhouse cultivated plants). Data collected on both experiments included the percentage of responsive explants (explants that formed buds), and the number of buds per explant. The addition of BAP showed the best organogenic response. In vitro germinated epicotyl segments and internodal segments are recommended as explants for sour orange in vitro organogenesis. Rooting of regenerated shoots was achieved without the need of auxin in the medium.

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Morphogenesis and tissue cultures of three citrus species

Cited by 63 publications

References 9 publications

Tissue culture parameters in sweet orange cultivars

Tissue culture parameters in sweet orange cultivars

An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

Sour orange bud regeneration and in vitro plant development related to culture medium composition and explant type

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