Sour orange bud regeneration and in vitro plant development related to culture medium composition and explant type

Abstract

-In order to evaluate the formation of adventitious buds and in vitro regeneration of sour orange plants (Citrus aurantium L.) two organogenesis-inducing experiments were conducted. In the fi rst experiment, the induction and in vitro regeneration of adventitious buds were tested on epicotyl and internodal segments under the infl uence of BAP or KIN associated with NAA. The second experiment evaluated the in vitro regeneration of sour orange plants related to different explant types (epicotyls segments, internodal segments of in vitro germinated plantlets and internodal segments of greenhouse cultivated plants). Data collected on both experiments included the percentage of responsive explants (explants that formed buds), and the number of buds per explant. The addition of BAP showed the best organogenic response. In vitro germinated epicotyl segments and internodal segments are recommended as explants for sour orange in vitro organogenesis. Rooting of regenerated shoots was achieved without the need of auxin in the medium.

In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis

“…The highest number of well-developed shoots was 4.2 shoots per explant and obtained with medium containing 0.5 mg lit -1 GA 3 . Silva et al (2010) by culturing Citrus aurantium epicotyl segments in MT (Murashige and Tucker, 1969) medium containing 1mglit -1 BAP and 0.3mglit -1 NAA were able to achieve 80.9% regeneration efficiency from which 3.1 buds per explants attained. The constituents of culture medium play an important role in the process of tissue differentiation.…”

In Vitro Mutagenesis and Propagation of Paulownia Tomentosa (Thumb) for Salt Tolerance

“…Other researchers reported that a gamma-ray dose of 0.1 kGy improved genetic variability but that a dose of 0.2 kGy increased differences in plant growth and production, as well as, molecular heterogeneity in soybeans and peanuts (Ferguson et al, 2004;Hanafiah et al, 2010). These gamma-ray effects have been linked to DNA structural rearrangements (breaks, transpositions, deletions, and so on) resulting from various types of DNA disruption (Silva et al, 2010). Miri et al (2009) utilized RAPD markers to identify genetic differences between induced banana mutant clones (Musa acuminata cv.…”

Organogênese in vitro e transformação genética de variedades de tangerina (Citrus reticulata Blanco e Citrus clementina hort. ex Tan.)

“…Os segmentos internodais utilzados nos experimentos de transformação genética com as três variedades não responderam ao processo de regeneração após a inoculação com A. tumefaciens e, desse modo, não foi possível a obtenção de plantas transgênicas com esse tipo de explante. Diferente do observado em trabalhos de organogênese in vitro com segmentos internodais com diferentes espécies de citros (GHORBEL et al, 1998;MOURA et al, 2001;ALMEIDA et al, 2002;SCHINOR et al, 2006;SILVA et al, 2010), nos quais foram obtidos resultados satisfatórios de regeneração in vitro, entretanto neste estudo, é possível afirmar que os segmentos internodais das três espécies estudadas sejam recalcitrantes ao processo de transformação genética nas condições descritas na metodologia.…”