In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis

Kaur, Sukhjit

doi:10.31018/jans.v8i2.865

Cited by 3 publications

(4 citation statements)

References 24 publications

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“…The earliest root induction was observed in MS medium supplemented with 1 mg/l NAA + 1 mg/l IAA and 1 mg/l NAA + 0.5 mg/l IBA. Similar observations were reported in Citrus jambhiri Lush (Kaur 2016). IBA has been observed to be an early root inducer in Citrus limon L. cv.…”

Section: Chhetri Et Alsupporting

confidence: 88%

“…When auxins were used alone, the highest number of roots were achieved in 1 mg/l IAA + 0.5 mg/l IBA. The combination of IAA and IBA produced highest number of roots in Citrus limon (Perez-Tornero et al 2009, Kaur 2016 and Citrus macrophylla (Tallon et al 2009). Early root initiation and higher number of roots were achieved when 1 mg/l NAA was used in MS media.…”

Section: Chhetri Et Almentioning

confidence: 99%

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Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees

Chhetri

Mathew

Pereira

2021

Plant Tissue Cult. & Biotech.

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A study was conducted to standardize a protocol for in-vitro direct regeneration and mass multiplication of Citrus indica Tanaka using shoot tip explants excised from mature trees. Shoot tips were inoculated in MS medium supplemented with varying concentrations and combinations of cytokinins and auxins. MS media when fortified with BAP 0.5 mg/l and 0.5 mg/l BAP + 1.0 mg/l Kn were found to be the best treatments for shoot initiation while MS supplemented with 1 mg/l IBA; 0.5 mg/l NAA + 0.5 mg/l IBA and 0.5 mg/l NAA + 0.5 mg/l IAA were the best treatments for root induction. Among the different media used for hardening, 100 % survivability was obtained when plantlets were hardened using vermicompost as the potting medium. Subsequently, these plantlets were transferred to larger pots and acclimatization was achieved gradually in outdoor conditions. Plant Tissue Cult. & Biotech. 31(1): 13-23, 2021 (June)

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Section: Chhetri Et Alsupporting

confidence: 88%

Section: Chhetri Et Almentioning

confidence: 99%

Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees

Chhetri

Mathew

Pereira

2021

Plant Tissue Cult. & Biotech.

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“…In vitro regeneration in C . jambhiri was successfully demonstrated deploying wide range of tissues and organs such as shoot tips [ 5 , 6 ], nodal explants [ 1 , 3 , 5 ], epicotyl segments [ 7 , 8 ], and cotyledons [ 9 , 10 ]. However, low multiplication rate and time-consuming culture processes are still prevalent problems in Citrus micropropagation [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

High efficient de novo root-to-shoot organogenesis in Citrus jambhiri Lush.: Gene expression, genetic stability and virus indexing

et al. 2021

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A protocol for high-frequency direct organogenesis from root explants of Kachai lemon (Citrus jambhiri Lush.) was developed. Full-length roots (~3 cm) were isolated from the in vitro grown seedlings and cultured on Murashige and Skoog basal medium supplemented with Nitsch vitamin (MSN) with different concentrations of cytokinin [6-benzylaminopurine, (BAP)] and gibberellic acid (GA3). The frequency of multiple shoot proliferation was very high, with an average of 34.3 shoots per root explant when inoculated on the MSN medium supplemented with BAP (1.0 mg L–1) and GA3 (1.0 mg L–1). Optimal rooting was induced in the plantlets under half strength MSN medium supplemented with indole-3-acetic acid (IAA, 0.5–1.0 mg L–1). IAA induced better root structure than 1-naphthaleneacetic acid (NAA), which was evident from the scanning electron microscopy (SEM). The expressions of growth regulating factor genes (GRF1 and GRF5) and GA3 signaling genes (GA2OX1 and KO1) were elevated in the regenerants obtained from MSN+BAP (1.0 mg L-1)+GA3 (1.0 mg L-1). The expressions of auxin regulating genes were high in roots obtained in ½ MSN+IAA 1.0 mg L-1. Furthermore, indexing of the regenerants confirmed that there was no amplicons detected for Huanglongbing bacterium and Citrus tristeza virus. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers detected no polymorphic bands amongst the regenerated plants. This is the first report that describes direct organogenesis from the root explant of Citrus jambhiri Lush. The high-frequency direct regeneration protocol in the present study provides an enormous significance in Citrus organogenesis, its commercial cultivation and genetic conservation.

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“…Bearss. Some workers 7,15 induced embryogenesis in C. reticulata and tried to produce direct in vitro plantlet through using epicotyl segment as explants.…”

Section: Introductionmentioning

confidence: 99%

Plantlet regeneration via nucellar embryogenesis in Citrus jambhiri

Singh¹,

Paliwal²,

Singh³

2020

Flora and Fauna

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To produce true-to-type and rapid multiplication micropropagation technique was utilized to the nucellar tissue with ovular halves of C. jambhiri. Nucellar tissues were cultured in a modified MS medium supplemented with different concentrations of 2ip viz. 0.25,0.50 mg l-1 alone and in combination of 0.50 mg l-1 NAA. Initiation of cell division and differentiation of proembryogenic tissue became apparent in first 80 days.These proembryos developed into embryos through subculturing in fresh medium. Low concentration of 0.25 2ip was found more suitable for the development of embryo producing large number of fully developed embryo in comparision to the embryo produced in the high concentration of 2ip (0.50 mg l-1) and in combination of 0.25 2ip and 0.50 mg l-1 NAA. Normally developed embryos in 0.25 2ip showed best germination in the fresh medium supplemented with 0.25 mg l-1 IAA, 100 ME mg l-1 and 5mg mg l-1 amino acids within 30 days as compared to other treatments. These germinated embryos were utilized for producing disease free saplings after hardening and nurturing in laboratory conditions. The disease free saplings thus produced can be used to establish new Citrus orchards within short time.

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In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis

Cited by 3 publications

References 24 publications

Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees

Micropropagation of Citrus indica Tanaka using Shoot tips from Mature Trees

High efficient de novo root-to-shoot organogenesis in Citrus jambhiri Lush.: Gene expression, genetic stability and virus indexing

Plantlet regeneration via nucellar embryogenesis in Citrus jambhiri

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