Monoiodoacetic acid induces arthritis and synovitis in rats in a dose- and time-dependent manner: proposed model-specific scoring systems

Udo, Mio; Muneta, Takeshi; Tsuji, Kunikazu; Ozeki, Nobutake; Nakagawa, Yusuke; Ohara, Toshiyuki; Saito, Ryusuke; Yanagisawa, Katsuhiko; Koga, Hideyuki; Sekiya, Ichiro

doi:10.1016/j.joca.2016.02.005

Cited by 108 publications

(141 citation statements)

References 30 publications

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“…According to another study of ours, 0.1 mg MIA induced punctate depressions on the surface of cartilage and cartilage erosion proceeded with time in Wistar rats [10]. In our current results, additional running advanced cartilage degeneration induced by 0.1 mg MIA in only the tibial cartilage, not in the femoral cartilage.…”

Section: Discussionsupporting

confidence: 69%

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Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats

Saito

Muneta

Ozeki

et al. 2017

BMC Musculoskelet Disord

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BackgroundIt is still debated whether strenuous running in the inflammatory phase produces beneficial or harmful effect in rat knees. We examined (1) the dropout rate of rats during a 30-km running protocol, (2) influences of strenuous running and/or low amounts of mono-iodoacetate injection on cartilage, and (3) the effect of strenuous running on synovitis.MethodsRats were forced to run 30 km over 6 weeks and the dropout rate was examined. One week after 0.1 mg mono-iodoacetate was injected into the right knee, rats were forced to run either 15 km or not run at all over 3 weeks, after which knee cartilage was evaluated. Synovium at the infrapatellar fat pad was also examined histologically.ResultsEven though all 12 rats run up to 15 km, only 6 rats completed 30 km of running. Macroscopically, 0.1 mg mono-iodoacetate induced erosion at the tibial cartilage irrespective of 15 km of running. Histologically, 0.1 mg mono-iodoacetate induced loss of cartilage matrix in the tibial cartilage, and an additional 15 km of strenuous running significantly exacerbated the loss. Synovitis caused by mono-iodoacetate improved after running.ConclusionsOnly 50% of rats completed 30 km of running because of foot problems. Strenuous running further exacerbated tibial cartilage erosion but did not influence synovitis induced by mono-iodoacetate.

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Section: Discussionsupporting

confidence: 69%

“…Intra-articular injection of mono-iodoacetate (MIA) induces arthritis and 1 mg MIA is often used to induce arthritis in rats. However, in this condition, both cartilage and bone are rapidly destructed [8–10]. Therefore, the use of this amount seems to be inappropriate for analyzing OA progression in OA models.…”

Section: Introductionmentioning

confidence: 99%

Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats

Saito

Muneta

Ozeki

et al. 2017

BMC Musculoskelet Disord

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“…In conclusion, the absence of WB asymmetry even 42 days after injection of 0.1 mg MIA indicates that this lower dose produced a discrete OA pain phenotype, rather than producing a more slowly developing OA model than that induced by 1 mg MIA injection 42 . Our study demonstrates that varying experimental procedures such as dose of MIA generates discrete OA pain phenotypes that may model pain phenotypes in human OA, which may aid the elucidation of the diverse mechanisms underlying OA pain and develop targeted treatments suitable for phenotypic subgroups.…”

Section: Discussionmentioning

confidence: 76%

Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA)

Nwosu

Mapp

Chapman

et al. 2016

Osteoarthritis and Cartilage

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SummaryObjectivesTo address the hypothesis that different types of established osteoarthritis (OA) pain behaviours have associations with different aspects of articular pathology, we investigated the relationship between structural knee joint pathology and pain behaviour following injection of a low vs a high dose of monosodium iodoacetate (MIA) in the rat.MethodsRats received a single intra-articular injection of 0.1 mg or 1 mg MIA or saline (control). Pain behaviour (hind limb weight bearing asymmetry (WB) and hindpaw withdrawal threshold (PWT) to punctate stimulation) was assessed. Cartilage and synovium were examined by macroscopic visualisation of articular surfaces and histopathology.ResultsBoth doses of MIA lowered PWTs, 1 mg MIA also resulted in WB asymmetry. Both doses were associated with cartilage macroscopic appearance, proteoglycan loss, abnormal chondrocyte morphology, increased numbers of vessels crossing the osteochondral junction, synovitis and macrophage infiltration into the synovium. PWTs were more strongly associated with chondrocyte morphology, synovitis and macrophage infiltration than with loss of cartilage surface integrity.ConclusionsBoth pain behaviours were associated with OA structural severity and synovitis. Differences in pain phenotype following low vs higher dose of MIA were identified despite similar structural pathology. OA structural pathology as traditionally measured only partially explains the MIA-induced pain phenotype.

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“…Inflammation stimulates fibrosis in AT, and macrophages can express extracellular matrix components 20. Of note, monoiodoacetate injection can induce OA and provoke IFP inflammation and fibrosis 21 22. IFP area and vascularisation are increased in the murine high-fat diet-induced OA model 10.…”

Section: Discussionmentioning

confidence: 99%

Knee and hip intra-articular adipose tissues (IAATs) compared with autologous subcutaneous adipose tissue: a specific phenotype for a central player in osteoarthritis

et al. 2017

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Monoiodoacetic acid induces arthritis and synovitis in rats in a dose- and time-dependent manner: proposed model-specific scoring systems

Cited by 108 publications

References 30 publications

Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats

Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats

Relationship between structural pathology and pain behaviour in a model of osteoarthritis (OA)

Knee and hip intra-articular adipose tissues (IAATs) compared with autologous subcutaneous adipose tissue: a specific phenotype for a central player in osteoarthritis

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