Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats

Saito, Ryusuke; Muneta, Takeshi; Ozeki, Nobutake; Nakagawa, Yusuke; Udo, Mio; Yanagisawa, Katsuhiko; Tsuji, Kunikazu; Tomita, Makoto; Koga, Hideyuki; Sekiya, Ichiro

doi:10.1186/s12891-017-1393-8

Cited by 6 publications

(8 citation statements)

References 23 publications

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“…However, only mild to moderate exercise is beneficial, as strenuous daily exercise aggravates OA ( Fig. 3 ) ( 115 ). Evidence on this matter from relevant animal studies is presented in Table III .…”

Section: Osteoarthritismentioning

confidence: 99%

Insight into the roles of melatonin in bone tissue and bone‑related diseases (Review)

Chen

et al. 2021

Int J Mol Med

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Bone-related diseases comprise a large group of common diseases, including fractures, osteoporosis and osteoarthritis (OA), which affect a large number of individuals, particularly the elderly. The progressive destruction and loss of alveolar bone caused by periodontitis is a specific type of bone loss, which has a high incidence and markedly reduces the quality of life of patients. With the existing methods of prevention and treatment, the incidence and mortality of bone-related diseases are still gradually increasing, creating a significant financial burden to societies worldwide. To prevent the occurrence of bone-related diseases, delay their progression or reverse the injuries they cause, new alternative or complementary treatments need to be developed. Melatonin exerts numerous physiological effects, including inducing anti-inflammatory and antioxidative functions, resetting circadian rhythms and promoting wound healing and tissue regeneration. Melatonin also participates in the health management of bone and cartilage. In the present review, the potential roles of melatonin in the pathogenesis and progression of bone injury, osteoporosis, OA and periodontitis are summarized. Furthermore, the high efficiency and diversity of the physiological regulatory effects of melatonin are highlighted and the potential benefits of the use of melatonin for the clinical prevention and treatment of bone-related diseases are discussed.

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Section: Osteoarthritismentioning

confidence: 99%

Insight into the roles of melatonin in bone tissue and bone‑related diseases (Review)

Chen

et al. 2021

Int J Mol Med

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“…We suggest that weight changes did not affect our experiments. According to the literature (Saito et al, 2017), we knew that not all rats could complete the exercise program due to intolerant intensity, especially in the OAH group. We found that all rats in the OAL group were able to complete the program; drop-out began at 3 week, and 80% of rats completed the program in the OAM group.…”

Section: Resultsmentioning

confidence: 99%

“…During the experimental period, rats that failed to run despite application of combined stimulation were defined as drop-outs and excluded from the experimental groups. Meanwhile, observation was required to ensure smooth execution of experiments, and the rats’ completion rate was calculated every week (Saito et al, 2017) (Figure 1C). Rats were sacrificed at the end of the experimental period.…”

Section: Methodsmentioning

confidence: 99%

The Therapeutic Effects of Treadmill Exercise on Osteoarthritis in Rats by Inhibiting the HDAC3/NF-KappaB Pathway in vivo and in vitro

Zhang

Yang

et al. 2019

Front. Physiol.

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Osteoarthritis (OA) is a disease characterized by non-bacterial inflammation. Histone deacetylase 3 (HDAC3) is a crucial positive regulator in the inflammation that leads to the development of non-OA inflammatory disease. However, the precise involvement of HDAC3 in OA is still unknown, and the underlying mechanism of exercise therapy in OA requires more research. We investigated the involvement of HDAC3 in exercise therapy-treated OA. Expression levels of HDAC3, a disintegrin and metalloproteinase with thrombospondin motifs-5 (ADAMTS-5), matrix metalloproteinase-13 (MMP-13), HDAC3 and nuclear factor-kappaB (NF-kappaB) were measured by western blotting, reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. Cartilage damage and OA evaluation were measured by hematoxylin and eosin staining and Toluidine blue O staining according to the Mankin score and OARSI score, respectively. We found that moderate-intensity treadmill exercise could relieve OA. Meanwhile, the expression of HDAC3, MMP-13, ADAMTS-5 and NF-kappaB decreased, and collagen II increased in the OA + moderate-intensity treadmill exercise group (OAM) compared with the OA group (OAG) or OA + high- or low-intensity treadmill exercise groups (OAH or OAL). Furthermore, we found the selective HDAC3 inhibitor RGFP966 could also alleviate inflammation in OA rat model through inhibition of nuclear translocation of NF-kappaB. To further explore the relationship between HDAC3 and NF-kappaB, we investigated the change of NF-kappaB relocation in IL-1β-treated chondrocytes under the stimulation of RGFP966. We found that RGFP966 could inhibit the expression of inflammatory markers of OA via regulation of HDAC3/NF-kappaB pathway. These investigations revealed that RGFP966 is therefore a promising new drug for treating OA.

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“…Regarding the metabolism of the articular cartilage, the balance and relationship between unloading, loading, and exercise have been still unclear, and some animal studies have demonstrated the influence of mechanical stress on OA progression using MIA injection, including treadmill running. However, the results were inconclusive 39,40 . To the best of our knowledge, the present study is the first to assess the influence of joint unloading on articular cartilage degeneration using rat model of OA in the PF and TF joints.…”

Section: Discussionmentioning

confidence: 99%

Joint unloading inhibits articular cartilage degeneration in knee joints of a monosodium iodoacetate-induced rat model of osteoarthritis

Takahashi

Matsuzaki

Kuroki

et al. 2019

Osteoarthritis and Cartilage

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s u m m a r yObjective: The aim of the study was to examine how mechanical unloading affects articular cartilage degeneration in the patellofemoral (PF) and tibiofemoral (TF) joints of a monosodium iodoacetate (MIA)induced rat model of osteoarthritis (OA). Design: The study involved 60 male rats. OA was induced by intra-articular injecting MIA into both knee joints. All animals were equally divided into two groups: sedentary (SE) and hindlimb unloading (HU) groups. Histopathological changes in the articular cartilage of the PF and TF joints were evaluated using the Osteoarthritis Research Society International (OARSI) score and modified Mankin score at 2 and 4 weeks after MIA injection. Results: In the SE and HU groups, representative histopathological changes in OA were detected in the PF and TF joints. The OARSI and modified Mankin scores for the PF and TF joints tended to increase over time after the injection of 0.2 mg or 1.0 mg of MIA in the SE and HU groups. Both the scores for the HU group were significantly lower than those for the SE group [OARSI score: P < 0.0001 (1.0-mg injection at 4 weeks); modified Mankin score: P ¼ 0.0116 (0.2-mg injection at 4 weeks); P ¼ 0.0004 and < 0.0001 (1.0-mg injection at 2 and 4 weeks, respectively)]. Conclusion: This study revealed new histological evidence that indicates that unloading condition suppresses articular cartilage degeneration and is beneficial in many areas of basal and clinical research involving OA.

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Strenuous running exacerbates knee cartilage erosion induced by low amount of mono-iodoacetate in rats

Cited by 6 publications

References 23 publications

Insight into the roles of melatonin in bone tissue and bone‑related diseases (Review)

Insight into the roles of melatonin in bone tissue and bone‑related diseases (Review)

The Therapeutic Effects of Treadmill Exercise on Osteoarthritis in Rats by Inhibiting the HDAC3/NF-KappaB Pathway in vivo and in vitro

Joint unloading inhibits articular cartilage degeneration in knee joints of a monosodium iodoacetate-induced rat model of osteoarthritis

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