Knee and hip intra-articular adipose tissues (IAATs) compared with autologous subcutaneous adipose tissue: a specific phenotype for a central player in osteoarthritis

Eymard, Florent; Pigenet, A.; Citadelle, D.; Tordjman, Joan; Foucher, Louise; Rose, Cindy; Flouzat-Lachaniette, Charles-Henri; Rouault, Christine; Clément, Karine; Bérenbaum, Francis; Chevalier, Xavier; Houard, Xavier

doi:10.1136/annrheumdis-2016-210478

Cited by 88 publications

(105 citation statements)

References 24 publications

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“…However, as of now, any article has been published on how to extract and identify exosomes in synovial tissue. Importantly, more and more researchers consider the synovial membrane and intra-articular fat pad as an anatomical unit in OA pathogenesis and pain, and inflammation of synovial membrane in the knee joint may play a central role in OA and affect not only cartilage but also intra-articular fat pad [7,29,30]. Moreover, previous team studies have shown that synovial inflammation may affect articular cartilage damage through the innate immune system, aggravating the course of OA [11].…”

Section: Discussionmentioning

confidence: 99%

Extraction and identification of synovial tissue-derived exosomes by different separation techniques

et al. 2020

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Objective: The aim of this study is to compare the efficiency of different separation techniques for extracting synovial tissue-derived exosomes. Methods: The synovial tissue discarded during knee arthroscopy or total knee arthroplasty surgery was collected from the Third Affiliated Hospital of Beijing University of Chinese Medicine. Ultracentrifugation (UC), filtration combined with size exclusion chromatography (SECF), and 8% polyethylene glycol (PEG) were used to extract synovial tissue-derived exosomes. Transmission electron microscopy (TEM), nanoparticle tracer analysis (NTA), and Western Blot (WB) were used to detect the morphology, particle size, and biomarker proteins (CD9, CD63, Flotillin-1, and calnexin) of exosomes. Results: The extracts of enriched round and discoid vesicles were successfully extracted with UC, SECF, and PEG. The results of TEM have shown that all three extraction methods can extract circular or elliptical vesicles with discand cup-shaped structures from the synovial tissue, with the diameter is about 30-150 nm. NTA suggested the main peaks of three groups of exosomes are around 100-120 nm, and the concentration of the three groups of exosomes was greater than 1 × 10 10 /ml. The results of WB showed that three positive protein markers (CD9, CD63, and Flotillin-1) were highly expressed in the suspension extracted by the three methods and low in the synovial tissue. However, the negative protein (calnexin) was highly expressed in synovial tissues and PEG group, while low in UC and SECF group. Conclusion: Morphology, particle size, and labeled protein marker detection confirmed that UC, SECF, and PEG can extract exosomes derived from synovial tissue; UC and SECF are more recommended for the extraction of synovial tissue-derived exosomes, which provides a methodological basis for studying the function and mechanism of synovial tissue exosomes in the future.

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Section: Discussionmentioning

confidence: 99%

Extraction and identification of synovial tissue-derived exosomes by different separation techniques

et al. 2020

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“…Higher level of IL-6 was associated with an increased risk of knee OA progression 64 , and serum levels of IL-6 and TNF-a were associated with joint space narrowing and cartilage loss in older adults 65 . A number of studies reported that IPFP secreted more IL-6 66,67 , IL-8, and prostaglandin E2 than ScAT in patients with knee OA 68 .…”

Section: Mechanisms Underlying the Link Between Adipose Tissue And Knmentioning

confidence: 99%

Systemic and local adipose tissue in knee osteoarthritis

Chang

Liao

et al. 2018

Osteoarthritis and Cartilage

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Knee osteoarthritis (OA) is the most common joint disease. Body adipose tissue has been shown to be related to the development and progression of knee OA. Among systemic adipose tissues, subcutaneous adipose tissue is significantly and negatively associated with muscle mass and forces, and could be related to the presence and progression of knee OA. Visceral adipose tissue is associated with increased cartilage loss and production of pro-inflammatory cytokines. Intra-muscular adipose tissue is associated with knee osteoarthritic changes, but it remains controversial if inter-muscular adipose tissue has a role to play in the pathogenesis for knee OA. Knee local adipose tissue such as infrapatellar fat pad (IPFP) can interact with neighbouring tissues, and may have a biphasic effect in knee OA. The underlying mechanisms for the roles of the systemic and local fat in knee OA could be related to biomechanical, metabolic, inflammatory factors and fat fibrosis, which may have a separated or combined effect on OA. Tissue engineering from systemic or local adipose tissue is a new research direction, and adipose tissue-derived stem cells from systemic or local adipose tissue may be beneficial for OA cartilage repair. Research on systemic and local adipose tissue would provide novel approaches for prevention and treatment of knee OA, but further studies are required to explore the roles of different adipose tissues in knee OA and the effects of stem cells derived from different adipose tissues on knee OA.

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“…During the past 10 years, IPFP has emerged as a new player in KOA for its pro‐inflammatory nature and ability to regulate cartilage metastasis and synovial inflammation . IPFP has been shown to have different phenotypes than subcutaneous fat tissue, with higher degrees of fibrosis and angiogenesis . The cause of fibrosis has not been elucidated, nor is it known whether cartilage degeneration is associated with IPFP fibrosis.…”

mentioning

confidence: 99%

“…5,6 IPFP has been shown to have different phenotypes than subcutaneous fat tissue, with higher degrees of fibrosis and angiogenesis. 7 The cause of fibrosis has not been elucidated, nor is it known whether cartilage degeneration is associated with IPFP fibrosis.…”

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confidence: 99%

Volume change in infrapatellar fat pad is associated not with obesity but with cartilage degeneration

Masaki

Takahashi

Hashimoto³

et al. 2019

Journal Orthopaedic Research

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Infrapatellar fat pad has been implicated in knee osteoarthritis. We examined whether infrapatellar fat pad volume is associated with quantitative cartilage changes using magnetic resonance imaging T1ρ mapping. Fifty‐eight knees of knee pain patients (19 men, 39 women, mean age 57.0 [range 29–85] years) who were conservatively managed and had >1 T1ρ mapping images taken over time were evaluated. We used three slices from the medial femoral and tibial cartilage; areas showing T1ρ values <130 ms and >50 ms were designated as having cartilage degeneration. Cases were categorized into three groups: Improvement, No Change, and Deterioration. Fat‐suppressed T2‐weighted sagittal magnetic resonance images were used for measuring infrapatellar fat pad volume. Percent change in infrapatellar fat pad volume was −5.01 ± 5.66%, −2.06 ± 4.92%, and 0.05 ± 6.09% in the Improvement (n = 22), No Change (n = 22), and Deterioration (n = 14) groups, respectively, demonstrating significantly reduced infrapatellar fat pad volume in the Improvement group (p < 0.05). Multivariate regression analyses revealed that the percent change in infrapatellar fat pad volume significantly affected T1ρ change category independent of age, sex, follow‐up period, baseline infrapatellar fat pad volume, and Kellgren‐Lawrence grade. Infrapatellar fat pad volume and obesity or body weight change showed no correlation. Infrapatellar fat pad volume was reduced in patients with improved quantitative cartilage assessment on magnetic resonance imaging T1ρ mapping. This is the first study demonstrating associations between quantitative cartilage changes and infrapatellar fat pad morphological changes, suggesting a detrimental role of infrapatellar fat pad volume in articular cartilage degeneration. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res

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Knee and hip intra-articular adipose tissues (IAATs) compared with autologous subcutaneous adipose tissue: a specific phenotype for a central player in osteoarthritis

Abstract: IFP but also SPFP and AFP (gathered under the term 'IAAT') may play a deleterious role in OA by affecting joint homeostasis because of their inflammatory phenotype and their close interaction with synovium in the same functional unit.

Cited by 88 publications

References 24 publications

Extraction and identification of synovial tissue-derived exosomes by different separation techniques

Extraction and identification of synovial tissue-derived exosomes by different separation techniques

Systemic and local adipose tissue in knee osteoarthritis

Volume change in infrapatellar fat pad is associated not with obesity but with cartilage degeneration

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