Monoamine receptors in human corneal epithelium and endothelium

Grueb, Matthias; Wallenfels-Thilo, Barbara; Denk, Oliver; Mielke, J.; Reinthal, Eva; Rohrbach, Jens Martin; Bartz‐Schmidt, Karl Ulrich

doi:10.1111/j.1600-0420.2005.00551.x

Cited by 26 publications

(32 citation statements)

References 16 publications

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“…However, the increase in CCT was statistically significant one month after initiation of tamsulosin treatment. The presence of α-1 receptors in freshly fixed human corneal epithelium and endothelium was demonstrated by Grueb et al 18 Moreover, direct radioligand binding studies indicate that intact corneal epithelial and endothelial cells exhibit α-1 adrenergic receptors. 19 Alpha-1 adrenergic receptors seem to be associated with regulation of inositol-1,4,5-tris-phospate formation.…”

mentioning

confidence: 80%

Influence of Tamsulosin on the Anterior Segment Parameters and Central Corneal Thickness

Palamar¹,

Emre²,

Turna³

et al. 2014

Turkiye Klinikleri J Med Sci

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To in ves ti ga te the ef fects of tam su lo sin (α-1 ad re ner gic re cep tor an ta go nist) on the ma in nu me ri cal pa ra me ters of an te ri or seg ment using Pen ta cam ro ta ting Sche immp flug came ra, and on bi o mec ha ni cal pa ra me ters using Re ic hert Ocu lar Res pon se Analy ser (ORA) in pa tients with be nign pros ta te hyperp la si a. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : In ad di ti on to full eye exa mi na ti on [best cor rec ted vi su al acu ity, ke ra to metry, in tra o cu lar pres su re (IOP) me a su red with Gold mann app la na ti on to no metry], Pen ta cam [cen tral cor ne al thick ness (CCT), an te ri or cham ber depth, ante ri or cham ber vo lu me, an te ri or cham ber ang le, pu pil di a me ter] and ORA (cor ne al hyste re sis, corne al re sis tan ce fac tor, cor ne al com pen sa ted IOP, Gold mann cor re la ted IOP) me a su re ments of 30 eyes of 15 ma le pa ti ents we re per for med be fo re and one month af ter tam su lo sin the rapy. Pa i red ttest and non-pa ra met ric Wil co xon test we re used for com pa ri sons. R Re e s su ul lt ts s: : Me an age in the study gro up was 61.50±6.10 (ran ge 47-69) ye ars. Af ter tam su lo sin tre at ment, CCT in cre a sed and this incre ment was sta tis ti cally sig ni fi cant (p=0.002). No ne of the ot her pa ra me ters that we re eva lu a ted sho wed sta tis ti cally sig ni fi cant dif fe ren ce af ter tam su lo sin use. C Co on nc c l lu u s si i o on n: : Tam su lo sin le ads to signi fi cant in cre ment in CCT. The ef fects of tam su lo sin on CCT sho uld be ta ken in to con si de ra ti on for pro per cli ni cal in ter pre ta ti on in pa ti ents using tam su lo sin.K Ke ey y W Wo or rd ds s: : Anterior eye segment; corneal topography; tamsulosin Ö ÖZ ZE ET T A Am ma aç ç: : Tam su lo si nin (α1 ad re ner jik re sep tör an ta go nis ti) be nign pros tat hi perp la zi li ol gular da Pen ta cam Sche immp flug ka me ra sis te mi ile çe ki len ön seg ment nü me rik pa ra met re le ri ne ve Re ic hert Ocu lar Res pon se Analy ser (ORA) ci ha zı ile kor ne a nın bi o me ka nik pa ra met re le ri üze ri ne olan et ki le ri nin de ğer len di ril me si. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Top lam 15 er kek ol gu nun 30 gö zü ne tamsu lo sin te da vi si ön ce si ve son ra sı tam of tal mo lo jik mu a ye ne (en iyi dü zel til miş gör me kes kin li ği, kera to met ri, Gold mann ap la nas yon to no met ri si ile öl çül müş gö zi çi ba sın cı (GİB), Pen ta cam [san tral kor ne a ka lın lı ğı (SKK), ön ka ma ra de rin li ği, ön ka ma ra hac mi, ön ka ma ra açı sı, pu pil ça pı] ve ORA (kor ne al his te re zis, kor ne al di renç fak tö rü, kor ne al dü zel til miş GİB, Gold mann dü zel til miş GİB) öl çüm le ri ya pıl dı. Kar şı laş tır ma lar için eş leş ti ril miş t-tes ti ve non-pa ra met rik Wil co xon test le ri kulla nıl dı. B Bu ul l g gu u l la ar r: : Ça lış ma gru bu nun or ta la ma ya şı 61,50±6,10 (47 ve 69 ara sın da) idi. SKK tam su losin te da vi si son ra sın da ön ce si ne gö re ar tış gös te...

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mentioning

confidence: 80%

Influence of Tamsulosin on the Anterior Segment Parameters and Central Corneal Thickness

Palamar¹,

Emre²,

Turna³

et al. 2014

Turkiye Klinikleri J Med Sci

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“…Although there was functional evidence for the presence of ␣ 2 -adrenergic receptors in corneal epithelial cells [12] , the ␣ 2 -receptor subtype had not been demonstrated in corneal epithelium for a long time. Recent studies have shown the presence ␣ 2A -adrenergic receptors in freshly fixed human corneal endothelium [14] . Our examination of bovine corneal epithelial and endothelial cells confirmed the presence of ␤ 1 -and ␤ 2 -adrenergic receptors in these tissues.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of ␤ -adrenergic receptors on corneal epithelial and endothelial cells has been established [8,9,14] . Corneal epithelial ␤ -adrenergic receptors have been associated with stimulation of adenylyl cyclase and cAMP-dependent protein kinase, as well as chloride secretion [13,22] .…”

Section: Discussionmentioning

confidence: 99%

“…Corneal epithelial ␤ -adrenoceptors have been associated with stimulation of adenylyl cyclase and cAMP-dependent protein kinase [13] . Recent studies revealed the presence of ␣ 1 -, ␤ 1 -and ␤ 2 -adrenergic receptors in human corneal epithelium, and the presence of ␣ 1 -, ␣ 2A -, ␤ 1 -and ␤ 2 -adrenergic receptors in human corneal endothelium [14] . Functional evidence for the expression of these receptors and their relative strength in the activation of the cAMP-PKA axis, however, has not yet been demonstrated.…”

Section: Pka In Corneal Epithelium and Endotheliummentioning

confidence: 99%

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Adrenergic Regulation of cAMP/Protein Kinase A Pathway in Corneal Epithelium and Endothelium

2008

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Purpose: The G-protein-coupled receptor/cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA) pathway is one of the most common and versatile signal pathways in eukaryotic cells. The aim of this study was to characterize subtypes of adrenergic G-protein-coupled receptors and their influence on cAMP concentration and PKA activity in bovine corneal epithelial and endothelial cells. Procedures: Adrenergic receptors and PKA were studied using polyclonal antibodies. cAMP concentration was determined with an enzyme immunoassay, and PKA activity was estimated by the kinases consumption of adenosine triphosphate. Results: In bovine corneal epithelial and endothelial cells, immunocytochemistry and Western blot were positive for α₁-, α_2A-, β₁- and β₂-adrenergic receptors. Stimulation of corneal epithelial and endothelial β-adrenoceptors with isoprenaline led to a dose-dependent increase in cAMP concentration and activation of PKA. Stimulation of corneal α_2A-adrenoceptors with brimonidine resulted in a dose-dependent decrease in cAMP concentration and the inhibition of PKA activity. Conclusions: In corneal epithelial and endothelial cells, β-adrenergic stimulation leads to activation of PKA via stimulation of adenylyl cyclase, and α_2A-adrenoceptor stimulation inhibits PKA activity via inhibition of adenylyl cyclase. Stimulation and inhibition of the corneal cAMP-PKA pathway may play a role in important corneal functions such as wound healing or homeostasis. Long-term therapy with α_2A-agonists or β-antagonists may influence these functions in a currently unknown way.

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“…15 Additionally, direct radioligand binding studies indicate that intact corneal epithelial and endothelial cells both exhibit α1 adrenergic receptors. 16 Alpha-1 adrenergic receptors seem to play a role in regulation of inositol-1,4,5-tris-phospate formation. 17 Although the physiological role of α1 adrenergic receptors and their second messengers phosphatidyl inositol and cyclic AMP in corneal epithelium and endothelium is not clear, they may be involved in the regulation of corneal homeostasis and fluid transportation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Tamsulosin on Corneal Biomechanical Features

Palamar¹,

Emre²,

Turna³

et al. 2016

Turkiye Klinikleri J Ophthalmol

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A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Tamsulosin (Flomax ® ) usage was reported to increase central corneal thickness (CCT). As CCT and ocular biomechanical parameters are closely related, this study was conducted to investigate the possible effects of tamsulosin on biomechanical parameters of cornea as measured with Reichert Ocular Response Analyser (ORA) in patients with benign prostate hyperplasia. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Besides full eye examination (best corrected visual acuity, intraocular pressure (IOP) measured with Goldmann applanation tonometry, CCT) ORA (corneal hysteresis, corneal resistance factor, corneal compensated IOP, Goldmann correlated IOP) measurements of 30 eyes of 15 male patients before and one month after tamsulosin therapy were performed. For comparisons paired t-test was used. R Re es su ul lt ts s: : Mean age in the study group was 61.50±6.10 (range 47-69) years. Pre-tamsulosin CCT was lower than post-tamsulosin values (pre-: 549.18±28.84 micrometers, post-: 556.77±24.86 micrometers; p=0.002). Although statistically insignificant corneal resistance factor after tamsulosin use was lower (pre-: 10.73±1.05 mmHg, post-: 9.45±1.51 mmHg; p=0.071). Measurements before and after tamsulosin treatment was not statistically different for any of the other parameters. C Co on nc cl lu us si io on n: : Tamsulosin seems not only to cause an increment on CCT, but also effect corneal resistance factor. The long term effects of tamsulosin on corneal biomechanical properties should further be investigated with larger group of patients. K Ke ey y W Wo or rd ds s: : Cornea; tamsulosin; eye Ö ÖZ ZE ET T A Am ma aç ç: : Tamsulosin (Flomax ® ) kullanımı sonrası merkezi kornea kalınlığında (MKK) artış olduğu bilinmektedir. Kornea kalınlığı ile korneanın biyomekanik özellikleri arasındaki yakın ilişki bulunduğundan, benign prostat hiperplazisi sebebiyle tamsulosin kullanan hastalarda korneada gelişebilecek muhtemel biyomekanik özellik değişikliklerinin Reicher Oküler Response Analiz cihaz (ORA) ile incelenmesi amacıyla bu çalışma planlanmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Tamsulosin kullanılmasından önce ve kullanımından 1 ay sonra 15 hastanın 30 gözünde tam oftalmolojik muayeneye (en iyi düzeltilmiş görme keskinliği, göz içi basınç ölçümü GİB) ilaveten MKK ölçümü ve ORA (korneal histerezis, korneal rezistans faktör, kornea kompanse GIB ve Goldmann korele GIB) ölçümleri yapılmış ve sonuçlar karşılaştırılmıştır. İstatistiksel analiz için eşleştirilmiş-t testi kullanılmıştır. B Bu ul lg gu ul la ar r: : Çalışma grubundaki hastaların ortalama yaşı 61,50±6,10 (range 47-69) yıl idi. Tamsulosin öncesi ve sonrasındaki MKK sırasıyla 549,18±28,84 ve 556,77±24,86 µm (p=0,002)idi. İn-celenen parametreler içerisinde hiçbir parametre için tedavi sonrasında anlamlı değişiklik izlenmemekle beraber yalnızca korneal rezistan faktör için , bir miktar azalma eğilimi olduğu izlenmiştir (pre-: 10,73±1,05 mmHg, post-:9,45±1,51 mmHg; p=0,071). S So on n...

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Monoamine receptors in human corneal epithelium and endothelium

Cited by 26 publications

References 16 publications

Influence of Tamsulosin on the Anterior Segment Parameters and Central Corneal Thickness

Influence of Tamsulosin on the Anterior Segment Parameters and Central Corneal Thickness

Adrenergic Regulation of cAMP/Protein Kinase A Pathway in Corneal Epithelium and Endothelium

Effect of Tamsulosin on Corneal Biomechanical Features

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