Molecular toxicity of Benzo(a)pyrene mediated by elicited oxidative stress infer skeletal deformities and apoptosis in embryonic zebrafish

Elfawy, Hasnaa A.; Anupriya, S.; Mohanty, Sasmita; Patel, Paritosh; Ghosal, Sayam; Panda, Pritam Kumar; Das, Biswadeep; Verma, Suresh K.; Patnaik, Srinivas

doi:10.1016/j.scitotenv.2021.147989

Cited by 37 publications

(16 citation statements)

References 52 publications

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“…Other studies have also shown induction of oxidative stress and apoptosis to be the mechanisms of B[ a ]P action. Elfawy et al [ 101 ] observed that B[ a ]P induced morphological and physiological abnormalities in the zebrafish skeleton (head, spine, and tail) due to induced oxidative stress leading to apoptosis.…”

Section: Metabolism Of B[ a ]Pmentioning

confidence: 99%

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Bukowska

Mokra

Michałowicz

2022

IJMS

159

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Benzo[a]pyrene (B[a]P) is the main representative of polycyclic aromatic hydrocarbons (PAHs), and has been repeatedly found in the air, surface water, soil, and sediments. It is present in cigarette smoke as well as in food products, especially when smoked and grilled. Human exposure to B[a]P is therefore common. Research shows growing evidence concerning toxic effects induced by this substance. This xenobiotic is metabolized by cytochrome P450 (CYP P450) to carcinogenic metabolite: 7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), which creates DNA adducts, causing mutations and malignant transformations. Moreover, B[a]P is epigenotoxic, neurotoxic, and teratogenic, and exhibits pro-oxidative potential and causes impairment of animals’ fertility. CYP P450 is strongly involved in B[a]P metabolism, and it is simultaneously expressed as a result of the association of B[a]P with aromatic hydrocarbon receptor (AhR), playing an essential role in the cancerogenic potential of various xenobiotics. In turn, polymorphism of CYP P450 genes determines the sensitivity of the organism to B[a]P. It was also observed that B[a]P facilitates the multiplication of viruses, which may be an additional problem with the widespread COVID-19 pandemic. Based on publications mainly from 2017 to 2022, this paper presents the occurrence of B[a]P in various environmental compartments and human surroundings, shows the exposure of humans to this substance, and describes the mechanisms of its toxicity.

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Section: Metabolism Of B[ a ]Pmentioning

confidence: 99%

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Bukowska

Mokra

Michałowicz

2022

IJMS

159

View full text Add to dashboard Cite

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“…The cardiomyocyte apoptosis was detected by AO staining ( Elfawy et al, 2021 , Zhu et al, 2015 ). Zebrafish embryos treated to 48 h after administration in each group were taken, the test solution of methanol extract of FAAC was removed, washed with culture water for 3 times, and then zebrafish embryos were stained with AO dye at 7.0 μg/mL in the dark for 30 min, and then washed twice with phosphate buffer (PBS, pH 7.4) ( Patel et al, 2020 , Verma et al, 2021a ).…”

Section: Methodsmentioning

confidence: 99%

Study on chemical constituents of Folium Artemisiae argyi Carbonisatum, toxicity evaluation on zebrafish and intestinal hemostasis

Wang

Shao

et al. 2022

Saudi Pharmaceutical Journal

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“…Epigenetic regulation of gene expression is a dynamic and reversible process ( 10 – 12 ). In biological and pathological processes, the related modifications are crucial for interpreting environmental signals and altering the expression of related genes ( 13 , 14 ). When these genes are dysregulated, diseases such as ESCC can occur ( 15 – 19 ).…”

Section: Introductionmentioning

confidence: 99%

METTL3 Is Associated With the Malignancy of Esophageal Squamous Cell Carcinoma and Serves as a Potential Immunotherapy Biomarker

Zhou

Guo

et al. 2022

Front. Oncol.

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Methyltransferase-like 3 (METTL3) is an RNA methyltransferase mediating N6 methyladenosine (m6A) modification. Its role in cancer pathogenesis and progression has attracted increasing attention. However, the immunological role, possible immune mechanism, and clinical significance of METTL3 in esophageal squamous cell carcinoma (ESCC) remain to be confirmed. The Tumor Genome Atlas (TCGA) provided clinical and transcriptome sequencing data for this study (162 tumor tissue samples and 11 normal tissue samples), while the Immunology Database and Analysis Portal (immport, https://www.immport.org/home) provided 2483 immune-related genes. METTL3 was substantially expressed in ESCC and linked to poor prognosis in ESCC, according to the findings. Functional analysis revealed that METTL3 is mainly involved in chromosomal homologous recombination and DNA mismatch repair processes, which could be potential mechanisms for tumor disease development and progression. Analysis on the TISIDB website shows that effector memory CD8 T cells, NK cells, neutrophils and other cells are highly correlated with METTL3 expression. We screened immune genes associated with METTL3 by Spearman’s analysis and performed functional analysis. These immune genes were mostly linked with immune processes, such as cytokine receptors, the MAPK signaling pathway, and natural killer cell-mediated cytotoxicity, indicating that METTL3 is a key molecule in the immune regulation of esophageal cancer. In addition, based on METTL3-related immune genes, we separated the patients into several subgroups and constructed a prognostic prediction model consisting of six immune genes. As an independent prognostic indicator for ESCC, the risk score of this model can be employed. A nomogram was also developed to accurately evaluate individual prognoses based on clinical indicators and risk scores. In summary, this study suggests that METTL3 is not only a potential pathogenic molecule for esophageal carcinogenesis and progression but also a potential biological marker for forecasting ESCC patient prognosis and could serve as a basis for clinical decision making.

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Molecular toxicity of Benzo(a)pyrene mediated by elicited oxidative stress infer skeletal deformities and apoptosis in embryonic zebrafish

Cited by 37 publications

References 52 publications

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Study on chemical constituents of Folium Artemisiae argyi Carbonisatum, toxicity evaluation on zebrafish and intestinal hemostasis

METTL3 Is Associated With the Malignancy of Esophageal Squamous Cell Carcinoma and Serves as a Potential Immunotherapy Biomarker

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