Hazelnut oil, extracted from hazelnuts, can be used as a functional food with many nutritional benefits. To completely assess its chemical constituents in hazelnut oil, one main cultivar of a flat-European hybrid hazelnut (C. heterophylla Fisch. × C. avellana L.)
cv. ‘Dawei’ was taken as the material, and a new optimized silyl-etherification derivatization was established by being followed with Gas chromatography-mass spectrometry (GC-MS). Meanwhile, an electron spin resonance (ESR) method was used to elucidate the scavenging capacity of
free radicals. In result, the filbertone (exclusive marker), 16 fatty acids (8 saturated and 8 unsaturated), 4 sterols and 5 other components were identified at once. Furthermore, hybrid hazelnut oil, filbertone and two sterols that contained in hazelnut oil were used to test the scavenge
free radical activities. The results showed that the hybrid hazelnut oil expressed meaningful IC50 values (DPPH: 66.39, hydroxyl: 66.64, alkyl: 118.82 and O2¯: 75.38 μg/mL). In conclusion, this study selected an effective derivatization method for the chemical
constituents detection, and provided detailed data, which laid an experimental foundation for quality control of a hybrid hazelnut oil. In addition, the free radical scavenging activity was observed, which provided a basis for the development of antioxidant products in hybrid hazelnut oil.
Rumex japonicus Houtt. (RJH) is a kind of Traditional Chinese Medicine (TCM) widely used in the treatment of serious skin diseases such as psoriasis. To further explore its bioactive components, the five-year root of RJH was used, the nuclear magnetic resonance (NMR) technique
was employed with DMSO-d6 solvent for chemical structures, the scavenging capacity of the free radicals (DPPH, alkyl, –OH, and ˙O−2) was evaluated by electron spin resonance (ESR) method. In the results, seven compounds that were wholly
characterized as anthraquinones (1 and 2), its c-glycosides (4–7), and flavonoid glycoside (3) especially for the hydrogen atoms from –OH groups located at phenol rings and glucose moieties. The compound 4 and 5 showed the
significant bioac-tivity with the IC50 values (DPPH: 23.34 and 23.25 μM; alkyl: 20.27 and 20.15 μM; –OH: 26.03 and 25.50 μM; and ˙O−2: 14.69 and 14.68 μM, respectively). In conclusion, the DMSO-d6
solvent could make active protons be expressed clearly for accurate assignments. The compounds 4 and 5 could be suggested to be applied for potential candidates for related drugs.
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