Molecular phylogenetic studies on the Diatrypaceae based on rDNA-ITS sequences

Abstract

Abstract:The order Diatrypales (Ascomycota) contains one single family, the Diatrypaceae. To obtain insight in the phylogenetic relationships within this family, the complete sequences of the ITS region (ITS1, 5.8S rRNA gene and ITS2) of 53 isolates from the five main genera in the family (Diatrype, Diatrypella, Cryptosphaeria, Eutypa and Eutypella) were determined and aligned for phylogenetic reconstruction. Sequence analysis revealed the presence of tandem repeated motifs 11 nucleotides-long, placed in homologous positions along the ITS1 region. Parsimony analysis established the existence of nine monophyletic groups and one branch with a single isolate of Eutypella quaternata. The phylogenetic relationships established by parsimony analysis did not correlate well with classical taxonomic schemes. None of the five genera included in this study was found to be monophyletic. The genera Diatrype, Eutypa and Cryptosphaeria each were divided into two groups. Isolates of Diatrype flavovirens appeared in a clade separated from the one that grouped Diatrype disciformis and the rest of Diatrype species. The Eutypa strains appeared distributed into two clades, one grouping Eutypa lata and related species (Eutypa armeniacae, Eutypa laevata, Eutypa petrakii), and anothAccepted for publication September 22, 2003. 1 Corresponding author. E-mail: fernandopelaez@merck.com er with the remaining species of the genus. Eutypella (excluding Eutypella quaternaria) appeared as an unstable monophyletic group, which was lost when the sequence alignment was subjected to neighbor-joining analysis. The genus Diatrypella was not associated with any monophyletic group, suggesting that the multisporate asci character has appeared several times during the evolution of the group. Overall, our study suggests the need to revise many of the concepts usually applied to the classification of members of the family.

Molecular Identification of Fomitiporia mediterranea and Eutypa lata/Libertella blepharis in Platanus × acerifolia

“…Two additional interspersed 11‐bp motifs were found, inside the same region, at positions 85C–95G and 108C–118G. All these repeats were modifications of the motif CTACCCTGTAG previously found in the ITS1 region of Dyatripaceae as tandem repeats or interspersed sequence patterns (Acero et al., 2004). All the Eutypa spp.…”

Diversity of diatrypaceous fungi associated with grapevine canker diseases in California

“…Phylogenetic analyses.-ITS sequences of Diatrypaceae published in Acero et al (2004) and Trouillas and Gubler (2004) were obtained from GenBank and included in the phylogenetic analysis. Taxa were selected to represent all major phylogenetic groups in the Diatrypaceae.…”

“…Excellent illustrations are available in an earlier treatment of Sphaeriaceae allantosporae by Berlese (1900), although Berlese's classification scheme is dated and many of the described species do not constitute valid names (Rappaz 1987, Vasilyeva andStephenson 2004). Acero et al (2004) proposed a first approach for a phylogenetic classification of these fungi based on ITS rDNA sequence data, showing divergent lineages and paraphyly within the family. Moreover this study did not support the typical classification scheme, which is based mostly on stromatal configuration as well as the morphology and disposition of perithecia (Glawe andRogers 1984, Rappaz 1987).…”

Diatrypasimilis australiensis, a novel xylarialean fungus from mangrove

“…Therefore whether D. australiensis is a representative of a new family in the Xylariales or a member of Diatrypaceae is not clear. Some genera in Diatrypaceae are polyphyletic and the molecular phylogeny is not in complete alignment with the morphology-based taxonomy of the family, as indicated by Acero et al (2004).…”