Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia

Ansharieta, Ribby; Ramandinianto, Sancaka Chasyer; Effendi, Mustofa Helmi; Plumeriastuti, Hani

doi:10.13057/biodiv/d220402

Cited by 26 publications

(36 citation statements)

References 21 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prevalence of the blaTEM genes in ESBLproducing E. coli was 3 (1.7%). This finding is in line with the research conducted by Ansharieta et al [21], who stated that E. coli contamination found in milk from dairy farms tends to encode the blaTEM gene in ESBL-producing E. coli bacteria. These results show that pathogenic E. coli originating from food of animal origin are also exposed to antibiotics and can transfer these genes to other pathogenic bacteria under certain conditions [45].…”

Section: Discussionsupporting

confidence: 92%

“…The ESBL-producing E. coli detected was further examined at a molecular level. Bacterial DNA was extracted using the QIAamp DNA Mini Kit (Promega) protocol according to Kristianingtyas et al [19], and the blaTEM gene was detected using the PCR method as described by Putra et al [20] and Ansharieta et al [21] as indicated in Table -1. After the amplification, products were visualized by exposure of the gel to ultraviolet light and subsequently photographed and documented using agel documentation system (Promega).…”

Section: Confirmation Test For Extended-spectrum Beta-lactamase (Esbl...mentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background and Aim: Raw milk can be a source of food-borne disease transmission and a medium for spreading antibiotic-resistant bacteria. Staphylococcus aureus and Escherichia coli are bacteria that have the pathogenic ability to attack host cells and are capable of harboring antibiotic-resistant genes. This study estimated the prevalence and antibiotic resistance of S. aureus and E. coli isolated from raw milk in East Java, Indonesia. Materials and Methods: Two hundred and fifty raw milk samples were collected from five dairy farms in East Java. S. aureus and E. coli were isolated using their respective selective media, whereas antibiotic susceptibility testing was performed using the Kirby–Bauer disk diffusion method. The methicillin-resistant S. aureus (MRSA) was confirmed using the oxacillin resistance screen agar test, and extended-spectrum beta-lactamase (ESBL)-producing E. coli was determined using the double-disk synergy test. The presence of mecA and blaTEM genes were screened by the polymerase chain reaction method. Results: Results indicated that the prevalence of S. aureus was 138 (55.2%) and that E. coli was 176 (70.4%). Of the 138 S. aureus isolated, 27 (19.6%) were MRSA, and among the 176 E. coli isolates identified, 3 (1.7%) were ESBL producers. The mecA gene was observed in 2 (7.4%) MRSA and all 3 (100%) ESBL-producing E. coli isolated harbored blaTEM genes. Conclusion: The presence of MRSA and ESBL-producing E. coli in raw milk is a serious public health threat, and public awareness should be raised about the dangers posed by these pathogenic organisms.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Confirmation Test For Extended-spectrum Beta-lactamase (Esbl...mentioning

confidence: 99%

Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The relative abundance of the ESBL producing E. coli in samples poultry has been shown to vary with geographic location (Wibisono et al 2020d). In this study, isolates including ESBL producing E. coli were detected by DDST (Ansharieta et al 2021a). Molecular identification showed that 25 (73,5%) samples of ESBL producing E. coli encoding iss gene, and 1 (2,9%) sample of ESBL producing E. coli encoding papC gene (Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…Infectious diseases involve the causative agent and host, as well as environmental factors. Diseases caused by Escherichia coli are disease agents that are often faced by all livestock farms, especially poultry farms, therefore knowledge, and information about disease incidence and prevention, control, and eradication efforts are needed (Putra et al 2020;Wibisono et al 2020b;Ansharieta et al 2021a).…”

Section: Introductionmentioning

confidence: 99%

Detection of virulence factor encoding genes on Escherichia coli isolated from broiler chicken in Blitar District, Indonesia

Effendi

FARIDAH²,

WIBISONO³

et al. 2022

Biodiversitas

Self Cite

View full text Add to dashboard Cite

Abstract. Efendi MH, Faridah HD, Wibisono FM, Wibisono FJ, Nisa N, Fatimah F, Ugbo EN. 2022. Detection of virulence factor encoding genes on Escherichia coli isolated from broiler chicken in Blitar District, Indonesia. Biodiversitas 23: 3437-3442. Broiler chicken is a source of protein that is widely consumed by the public. However, broiler chicken production sometimes decreases due to infectious diseases such as colibacillosis caused by pathogenic Escherichia coli possessing virulence genes. Virulence factors function to facilitate colonization and invasion of host cells to cause disease. The presence of these virulence factors is encoded by various genes such asthe increased serum survival gene and P fimbriae gene which plays a role in surface adhesion. The present study aims to detect the presence of virulence genes from extended-spectrum beta-lactamase (ESBL) producing E.coli isolated from broiler chickens in the Blitar District. A total of 110 cloacal swabs collected by systematic random sampling from broiler poultry farms in four different sub-districts were screened for ESBL-producing E. coli and virulence genes by phenotypic and molecular methods, respectively. Out of 110 E.coli recovered, 95 (86.4%) were observed to show a high level of resistance to the tested antibiotics, and 34 (35.7%) were ESBL-producers. Among ESBL producing E. coli isolates, 22 (73.5%) and 1 (2.9%) were found to have the iss and papC gene virulence factors, respectively using the polymerase chain reaction (PCR) method. The results of this study indicate that virulence genes can be found in E. coli from poultry farms. The iss gene is the most predominant virulence gene. The reportof these virulence factors in E. coli isolated from broiler could impose a serious potential public health problem.

show abstract

“…Asia is one of the epicenters of antibiotic resistance, there are a surprising number of drug-resistant strains, including K. pneumoniae, and a large number of acquired Gramnegative MDR strains have been found (Jean and Hsueh 2011). AMR can be disseminated rapidly through a variety of possible pathways, including foodborne pathogens, insects, wastewater, pets, food producers or wild animals Ansharieta et al 2021). In particular, more than 25 outbreaks of human infectious disease outbreaks were reported, 11 associated with animals on farms, and zoos from 1990 to 2000 (Bender and Shulman 2004).…”

Section: Have Been Found To Be Infected With Esblproducingmentioning

confidence: 99%

Molecular detection of extended spectrum ?-lactamase producing Klebsiella pneumoniae from wild deer

et al. 2022

Self Cite

View full text Add to dashboard Cite

Abstract. Riwu KHP, Rantam FA, Effendi MH, Khairullah AR, Widodo A, Kurniawan SC, Ugbo EN. 2022. Molecular detection of extended spectrum ?-lactamase producing Klebsiella pneumoniae from wild deer. Biodiversitas 23: 4256-4262. Antimicrobial resistance (AMR) has become a severe threat worldwide and its remote cause remains unclear in most parts due to the global emergence of new resistance mechanisms by microorganisms. This research aimed to identify the presence of extended-spectrum ?-lactamase (ESBL) Klebsiella pneumoniae from wild deer feces in several places in East Java and East Nusa Tenggara. This study used 129 fresh feces samples collected from wild deer and analyzed for the presence of K. pneumoniae using standard microbiological techniques. An antibiotic sensitivity test was performed using Kirby-Bauer Disc Diffusion Method. The isolates were confirmed to the ESBL producer by double disk test and ESBL genes were identified by PCR. Nine samples (6.98%) were K. pneumoniae positive out of the 129 wild deer fresh feces. Among the nine K. pneumoniae isolates studied 3 (33.33%) were multidrug resistant, East Java (1 isolate) and East Nusa Tenggara (2 isolates). All the multidrug-resistant K. pneumoniae isolates were ESBL positive and also harbored blaTEM gene. The presence of MDR, and ESBL-producing K. pneumoniae isolates was confirmed in wild deer and this explained that wild deer should be considered a source of transmission of MDR and ESBL to public health.

show abstract

Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia

Cited by 26 publications

References 21 publications

Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia

Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia

Detection of virulence factor encoding genes on Escherichia coli isolated from broiler chicken in Blitar District, Indonesia

Molecular detection of extended spectrum ?-lactamase producing Klebsiella pneumoniae from wild deer

Contact Info

Product

Resources

About