Detection of virulence factor encoding genes on Escherichia coli isolated from broiler chicken in Blitar District, Indonesia

Abstract

Abstract. Efendi MH, Faridah HD, Wibisono FM, Wibisono FJ, Nisa N, Fatimah F, Ugbo EN. 2022. Detection of virulence factor encoding genes on Escherichia coli isolated from broiler chicken in Blitar District, Indonesia. Biodiversitas 23: 3437-3442. Broiler chicken is a source of protein that is widely consumed by the public. However, broiler chicken production sometimes decreases due to infectious diseases such as colibacillosis caused by pathogenic Escherichia coli possessing virulence genes. Virulence factors function to facilitate colonization and invasion of host cells to cause disease. The presence of these virulence factors is encoded by various genes such asthe increased serum survival gene and P fimbriae gene which plays a role in surface adhesion. The present study aims to detect the presence of virulence genes from extended-spectrum beta-lactamase (ESBL) producing E.coli isolated from broiler chickens in the Blitar District. A total of 110 cloacal swabs collected by systematic random sampling from broiler poultry farms in four different sub-districts were screened for ESBL-producing E. coli and virulence genes by phenotypic and molecular methods, respectively. Out of 110 E.coli recovered, 95 (86.4%) were observed to show a high level of resistance to the tested antibiotics, and 34 (35.7%) were ESBL-producers. Among ESBL producing E. coli isolates, 22 (73.5%) and 1 (2.9%) were found to have the iss and papC gene virulence factors, respectively using the polymerase chain reaction (PCR) method. The results of this study indicate that virulence genes can be found in E. coli from poultry farms. The iss gene is the most predominant virulence gene. The reportof these virulence factors in E. coli isolated from broiler could impose a serious potential public health problem.

Molecular detection of hlyF gene on multidrug resistance of avian pathogenic Escherichia coli isolated from ducks on wet markets of Surabaya, Indonesia

“…Ducks originating from live markets in Indonesia are still sold traditionally, exacerbated by the lack of biosecurity and sanitation implemented by workers in the markets (Effendi et al 2022). The ducks can be infected by bacteria, both pathogenic and non-pathogenic, including Pasteurella multocida, Salmonella enterica, and Escherichia coli (Wei et al 2013;Soman et al 2014;Li et al 2016;Abd El-Ghany, 2023).…”

“…Interpretation of the results of the diameter of the inhibition zone is adjusted based on CLSI (CLSI 2020; Effendi et al 2021;Yanestria et al 2022). Isolates are declared multidrug-resistant (MDR), where bacteria are resistant to three or more different classes of antibiotics (Kissinga et al 2018;Effendi et al 2022;Putri et al 2023).…”

Integration of water, sanitation, and hygiene program with biosecurity: A One Health approach to reduce the prevalence and exposure of antibiotic-resistant bacteria in the livestock community

“…In Asia, India reported an occurrence rate of up to 75% [44], whereas Qatar reported 18% [45]. In Indonesia, 35.7% of broiler farms [46] and 52% in Sri Lanka [47] were detected.…”