Molecular detection of virulence genes in Klebsiella Pneumoniae clinical isolates from Kurdistan Province, Iran

Abstract

Introduction: The purpose of this study was to detect ybtS, entB, mrkD, magA, kfu, iutA, rmpA and K2 genes in extended-spectrum beta-lactamase (ESBL) - and non-ESBL producing Klebsiella pneumoniae.
 Methods: To this end, 70 K. pneumoniae isolates were selected from hospitals of Kurdistan Province, Iran. The ESBL phenotype was conducted utilizing the disc diffusion technique in accordance with CLSI procedures. Detection of virulence factor genes was performed by the PCR in the ESBL and non-ESBL isolates.
 Results: Sixty-two (88.6%) isolates of K. pneumoniae were ESBL producers. Further, entB had the most frequency in all the isolates. There were no significant differences between ESBL production and the presence of ybt S, entB, mrkD, magA, kfu, iutA, rmpA and K2 genes and the presence of these genes and variables such as presence of sex, clinical specimen type, and hvKP phenotype among the ESBL and non-ESBL K. pneumoniae isolates.
 Conclusion: In conclusion, in other studies, K. pneumoniae strains were separated from liver abscesses and the magA gene was frequently present; however, in our study, the K. pneumoniae strains were separated from various clinical specimens and the magA gene had low frequency.

Analysis of antibiotic resistance and virulence patterns inKlebsiella pneumoniaeisolated from human urinary tract infections in India

“…They could not detect any virulence genes in 2Á7% of their isolates. The levels of virulence genes were comparable with previous works by different authors where a wide range of values was observed and reported (Jian- Li et al 2017;Shakib et al 2018;Ali and Al-kakei, 2019). However, the difference among the values could be due to different geographical location and sample size.…”

Molecular Detection Of Some Capsulargenes Of Klebsiella Pneumoniae Isolated From Clinical Samples

“…The serotype k1,k3 , k5 and k20 genes were not detected in K. pneumonia isolates . Regarding serotype K2 gene, it was identified in 12.9 % isolates of our study but was not detected in study of Hamam et al [10] and Shakib et al [14] . However, it was reported in 6.7 % in the study of Chen et al [5] Serotype K54 detected in 37.8 % isolates of our study and 2.1 % in the isolates of study by Chen et al [5] .Capsule serotype k57 in 25.8 % isolates of our study, while in the study of Ssekatawa et al [18] there was no detection of K57 serotype gene.…”

Has resistance to chlorhexidine increased among clinically-relevant bacteria? A systematic review of time course and subpopulation data

“…pneumoniae isolated in the 1940s and 1950s prior to the widespread use of antibiotics [ 32 , 33 ], were compared to more recently isolated strains [ 34 ]. Wand, et al [ 32 ] and Shakib et al [ 35 ] both reported similar patterns in expression of virulence and resistance factors with differences between pre-antibiotic and more recent strains supporting the idea that important virulence and resistance factors that were infrequently expressed in pre-antibiotic era K . pneumoniae strains are now commonly expressed.…”