Molecular detection of Sarcocystis species in slaughtered sheep by PCR–RFLP from south-western of Iran

Abstract

Sarcocystis spp. are the cyst forming protozoan parasites that prevalent in livestock all around the world. In the presented work, we examined 40 macroscopic and 40 microscopic sarcocysts from Khouzestan and Lorestan provinces, south-western Iran, utilizing PCR-RFLP based on amplification of 18S rRNA gene. Using AvaI, HindII, TaqI and EcoRI restriction enzymes the results represented Sarcocystis gigantea in both macroscopic and microscopic cysts. This result supports the importance of molecular investigations on characterization of Sarcocystis species when we aimed to assess the reliable control and preventive programs.

Oesophageal sarcocystosis observed at slaughter provides a reliable and efficient proximate measure of Toxoplasma gondii seroprevalence in sheep

“…In Australia, macroscopic sarcocystosis is known to occur in all sheep‐producing states (Tasmania, Victoria, New South Wales, South Australia, Western Australia, Queensland), although finer details of its distribution within these states is not well known, other than for South Australia . Outside of Australia, we found evidence of macroscopic sarcocystosis existing in at least 10 countries . As T. gondii is known to have a worldwide distribution, this suggests that these two parasitoses likely co‐occur in sheep worldwide.…”

Identification of Sarcocystis spp. in One-humped Camels (Camelus dromedarius) from Riyadh and Dammam, Saudi Arabia, via Histological and Phylogenetic Approaches

“…For all tissues, approximately 20 g were minced before digestion for a period of 30 min in 100 mL at a temperature of 40 • C; the digestion solution comprised pepsin (1.3 g), HCl (3.5 mL) and NaCl (2.5 g) in distilled water (500 mL) [25]. After digestion, the mixture was centrifuged at 3500× g for 3 min, followed by Giemsa staining and optical microscopy evaluation [26].…”

Os parasitas de ovinos

“…A PCR-RFLP é amplamente utilizada e considerada como método molecular rápido, pouco dispendioso e acurado para discriminar diferentes protozoários, bem como Sarcocystis spp. (Ellis et al, 1995;Tenter, 1999;Motamedi et al, 2011, Hamidinejat et al, 2014Formisiano et al, 2013).…”