Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam

Borah, Munmi; Kumar, Rupesh; Siddappa, Sundaresha

doi:10.23910/ijep/2019.6.3.0320

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…Borah et al [41] detected the presence of CMV in Capsicum chinense Jacq. in India by RT-PCR and prepared the rst report on molecular characterization of the virus.…”

Section: Discussionmentioning

confidence: 99%

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Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

AKDURA

Kiliç

2022

Preprint

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Background Tomato (Lycopersicum esculentum Mill.) and pepper (Capsicum anuum L.) are the host species for the Cucumber mosaic virus (CMV) and Tomato mosaic virus (ToMV). This article aims to report the detection of serolocigally and molecularly and identification of genetic diversity these viruses in Hakkari province, Turkey.Methods and results A total of 184 leaf samples were collected from tomato and pepper plants that showed virus-like symptoms. Sample collection was carried out in Hakkari province in October, 2020. CMV and ToMV factors were investigated by Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). DAS-ELISA test showed that 106 (57.60%) of the samples were infected with one or more viruses. For phylogenetic analyses, 10 samples which were positive for CMV and ToMV were tested using Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. Coat protein (CP) and replicase genes of Hakkari CMV and ToMV isolates were amplified by RT-PCR using specific primers. The resulting DNA sequences were deposited in NCBI GenBank (Accession Nos: OM286731, OM286732, OM286733, OM286734, OM418629). When the replicase and coat protein gene sequences of four ToMV isolates and one CMV isolate were compared with the isolates in GenBank, a nucleotide homology of 98.1-99.6% and 91.7-99.4% were found, respectively. Phylogenetic analysis revealed a strong similarity between Hakkari ToMV and CMV isolates and other Turkish isolates. Consistent with the molecular studies conducted in Turkey on CMV and ToMV, the isolates detected in this study also clustered in subgroup Ia. Conclusions With this study, CMV and ToMV factors were serologically and molecularly detected for the first time in Hakkari and the first nation-wide report for ToMV replicase sequences in infected tomato and peppers were submitted.

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“…Borah et al [41] detected the presence of CMV in Capsicum chinense Jacq. in India by RT-PCR and prepared the rst report on molecular characterization of the virus.…”

Section: Discussionmentioning

confidence: 99%

“…CMV is one of the most harmful viruses causing disease outbreaks in agricultural products all around the world [21]. Studies to be conducted in other countries will be helpful in understanding the epidemiology of CMV and in designing an appropriate management program [41].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

AKDURA

Kiliç

2022

Preprint

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“…Tospoviruses (CaCV and GBNV) infected chilli plants showed concentric rings on mature leaves and chlorotic spots on younger leaves 12 . Indeed, CMV infected chilli plants showed mosaic mottling, puckering, shoe string or filiformity and stunted growth 26 , 27 . Based on symptomology, symptomatic leaf samples of mosaic mottling along with concentric chlorotic ring spots and mosaic with veinal necrosis were suspected for mixed infection.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, mixed infections tend to affect virulence, accumulation, symptomology and host range which ultimately results in unpredictable pathological consequences 24 , 25 . Moreover, virus interaction in host plant hews the evolutionary dynamic of viral populations 26 . Unraveling the viruses concerned with mixed infections is vital for better understanding of the ecology and evolution of viral diseases which are essential for viral disease prevention and formulating management strategies 25 .…”

Section: Introductionmentioning

confidence: 99%

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Vinodhini

Rajendran

Abirami

et al. 2021

Sci Rep

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Cucumo- and tospoviruses are the most destructive viruses infecting hot pepper (chilli). A diagnostic survey was conducted to assess the prevalence of cucumo and tospoviruses in chilli growing tracts of Tamil Nadu. Infected plants showing mosaic with chlorotic and necrotic rings, veinal necrosis, mosaic mottling, leaf filiformity and malformation were collected. Molecular indexing carried out through reverse transcription polymerase chain reaction (RT-PCR) with coat protein gene specific primer of Cucumber mosaic virus (CMV) and tospovirus degenerate primer corresponding to the L segment (RdRp). Ostensibly, amplifications were observed for both CMV and tospoviruses as sole as well for mixed infections. The sequence analysis indicated that the Capsicum chlorosis virus (CaCV) and Groundnut bud necrosis virus (GBNV) to be involved with CMV in causing combined infections. The co-infection of CMV with CaCV was detected in 10.41% of the symptomatic plant samples and combined infection of CMV with GBNV was recorded in around 6.25% of the symptomatic plants surveyed. The amino acid substitution of Ser129 over conserved Pro129 in coat protein of CMV implies that CMV strain involved in mixed infection as chlorosis inducing strain. Further, the electron microscopy of symptomatic plant samples explicated the presence of isometric particles of CMV and quasi spherical particles of tospoviruses. This is the first molecular evidence for the natural co-existence of chlorosis inducing CMV strain with CaCV and GBNV on hot pepper in India.

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Induction of RNA-Mediated Resistance Against Cucumber Mosaic Virus on Exogenous Application of dsRNA

Saikia

Routhu

Borah

2023

Technologies for Rural Development

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Molecular Detection and Phylogenetic Analysis of Cucumber Mosaic Virus Infecting Bhut Jolokia (Capsicum chinense Jacq.) of Assam

Cited by 6 publications

References 8 publications

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Induction of RNA-Mediated Resistance Against Cucumber Mosaic Virus on Exogenous Application of dsRNA

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