Co-existence of chlorosis inducing strain of Cucumber mosaic virus with tospoviruses on hot pepper (Capsicum annuum) in India

Abstract

Cucumo- and tospoviruses are the most destructive viruses infecting hot pepper (chilli). A diagnostic survey was conducted to assess the prevalence of cucumo and tospoviruses in chilli growing tracts of Tamil Nadu. Infected plants showing mosaic with chlorotic and necrotic rings, veinal necrosis, mosaic mottling, leaf filiformity and malformation were collected. Molecular indexing carried out through reverse transcription polymerase chain reaction (RT-PCR) with coat protein gene specific primer of Cucumber mosaic virus (CMV) and tospovirus degenerate primer corresponding to the L segment (RdRp). Ostensibly, amplifications were observed for both CMV and tospoviruses as sole as well for mixed infections. The sequence analysis indicated that the Capsicum chlorosis virus (CaCV) and Groundnut bud necrosis virus (GBNV) to be involved with CMV in causing combined infections. The co-infection of CMV with CaCV was detected in 10.41% of the symptomatic plant samples and combined infection of CMV with GBNV was recorded in around 6.25% of the symptomatic plants surveyed. The amino acid substitution of Ser129 over conserved Pro129 in coat protein of CMV implies that CMV strain involved in mixed infection as chlorosis inducing strain. Further, the electron microscopy of symptomatic plant samples explicated the presence of isometric particles of CMV and quasi spherical particles of tospoviruses. This is the first molecular evidence for the natural co-existence of chlorosis inducing CMV strain with CaCV and GBNV on hot pepper in India.

Detection of orthotospoviruses in medicinal plants in China

“…It can, therefore, be inferred that chlorotic rings and line patterns observed in Apocynaceae plants may be an indication of orthotospovirus infection. In 2021, J. Vinodhini reported the co‐existence of chlorosis inducing strain of Cucumber mosaic virus with orthotospoviruses (CaCV and Groundnut bud necrosis virus, GBNV) on Capsicum annuum in India (Vinodhini et al, 2021). The observed symptoms may have been caused by orthotospoviruses and the other viruses.…”

İzmir ilinde domates ve biber bitkilerini etkileyen tomato spotted wilt virus (TSWV) ve cucumber mosaic virus (CMV) etmenlerinin moleküler karakterizasyonu

“…It is noteworthy that TSWV and CMV are prevalent throughout the Mediterranean Basin (Roggero et al, 2002;Yardımcı & Eryiğit, 2006;Debreczeni et al, 2015) and recently in Asia (Jiang et al, 2017;Vinodhini et al, 2021), Africa (Karavina et al, 2016;Waweru et al, 2020) and North America (Lin et al, 2003;Batuman et al, 2017). Furthermore, the rapid increase of new variants arise makes difficult to control the viruses (Roggero et al, 2002).…”

Detection of Banana Mild Mosaic Virus in Musa In Vitro Plants: High-Throughput Sequencing Presents Higher Diagnostic Sensitivity Than (IC)-RT-PCR and Identifies a New Betaflexiviridae Species

“…The infection often results in symptomless plants of Musa spp., and the virus has a worldwide distribution, which can explain its high prevalence [9,10]. The impact of BanMMV seems somewhat mild on banana crop, although mixed infections (mostly with BSV and BBrMV) can lead to severe leaf necrosis [9,11,12]. The virus displays a very high molecular diversity [13].…”