Molecular characterization, tissue distribution and feeding related changes of NUCB2A/nesfatin-1 in Ya-fish (Schizothorax prenanti)

Lin, Fangjun; Zhou, Chaowei; Chen, Hu; Wu, Hongwei; Xin, Zhiming; Ju, Liu; Gao, Yundi; Yuan, Dengyue; Wang, Tao; Wei, Ran; Chen, Defang; Yang, Shiyong; Wu, Yan; Pu, Yundan; Li, Zhiqiong

doi:10.1016/j.gene.2013.12.031

Cited by 49 publications

(41 citation statements)

References 51 publications

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“…A more recent report also found two NUCB2 genes in Ya fish (Lin et al, 2014). Here, we determined that NUCB2A and NUCB2B gene sequences in zebrafish are highly similar to NUCB2 from other fishes and mammals (Fig.…”

Section: Discussionsupporting

confidence: 68%

“…Recently, Lin et al (2014) reported a similar increase in NUCB2A mRNA expression in the intestine and hypothalamus after feeding. NUCB2A mRNA expression in the hepatopancreas increased upon food deprivation and after short term fasting in goldfish (Gonzalez et al, 2010) and Ya fish (Lin et al, 2014). These results provide evidence supporting the potential metabolic roles of NUCB2 in fish.…”

Section: Introductionmentioning

confidence: 71%

“…Moreover, peripheral or central injection of native nesfatin-1 caused a significant reduction in food intake of goldfish (Gonzalez et al, 2010). Recently, Lin et al (2014) reported a similar increase in NUCB2A mRNA expression in the intestine and hypothalamus after feeding. NUCB2A mRNA expression in the hepatopancreas increased upon food deprivation and after short term fasting in goldfish (Gonzalez et al, 2010) and Ya fish (Lin et al, 2014).…”

Section: Introductionmentioning

confidence: 82%

“…Proteolytic processing of NUCB2 by prohormone convertases results in the N-terminal fragment, nesfatin-1 (residues 1-82) and two C-terminal peptides, nesfatin-2 (residues 85-163) and nesfatin-3 (residues 166-396) (Oh-I et al, 2006;Gonzalez et al, 2010Gonzalez et al, , 2012. In fish, it is likely that two isoforms of NUCB2 (NUCB2A and NUCB2B) exist and nesfatin-1 has been identified in goldfish (Carassius auratus) (Gonzalez et al, 2010) and Ya fish (Schizothorax prenanti) (Lin et al, 2014). NUCB2A mRNA is expressed in the brain (hypothalamus), pituitary, liver, hepatopancreas, intestine and gonads of goldfish (Gonzalez et al, 2010) and Ya fish (Lin et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In fish, it is likely that two isoforms of NUCB2 (NUCB2A and NUCB2B) exist and nesfatin-1 has been identified in goldfish (Carassius auratus) (Gonzalez et al, 2010) and Ya fish (Schizothorax prenanti) (Lin et al, 2014). NUCB2A mRNA is expressed in the brain (hypothalamus), pituitary, liver, hepatopancreas, intestine and gonads of goldfish (Gonzalez et al, 2010) and Ya fish (Lin et al, 2014). More recently, nesfatin-1 was detected in the circulation of female rainbow trout (Caldwell et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain

Hatef

Shajan

Unniappan

2015

General and Comparative Endocrinology

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Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain

Hatef

Shajan

Unniappan

2015

General and Comparative Endocrinology

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Expression profiling and inflammatory activation analysis of high‐mobility group box 1 in Schizothorax prenanti

Chen

Hong

et al. 2022

J Aqua Anim Hlth

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Objective High‐mobility group box 1 (HMGB1) is a highly conserved nuclear protein and participates in the immune response to pathogens in bony fish. In this study, the structure and function of HMGB1 in the cyprinid fish Schizothorax prenanti (SpHMGB1) were investigated. Methods The spatial structure of SpHMGB1 was predicted by CPHmodels. Quantitative reverse transcription PCR was used to detect the mRNA of SpHMGB1 in different tissues and Streptococcus agalactiae infection. The macrophage was treated with synthetic SpHMGB1‐B box peptide to analyze the inflammatory activity. Result Structurally, SpHMGB1 had the conserved A box, B box, and acid tail compared with Zebrafish Danio rerio and mice Mus musculus. SpHMGB1 was universally expressed in various tissues, with the highest expression in the middle kidney. In vivo, SpHMGB1 was significantly induced in response to Streptococcus agalactiae infection in the blood and spleen. Synthetic SpHMGB1‐B box peptide activated respiratory burst and up‐regulated the messenger RNA expression of interleukin‐1β, tumor necrosis factor α, interleukin‐10, interferon regulatory factor 1, interferon regulatory factor 7, C‐X‐C motif chemokine ligand 11‐1, C‐X‐C motif chemokine ligand 11‐2, and toll‐like receptor 4 in macrophages. Conclusion This study suggested that SpHMGB1 participated in the response to bacterial pathogens and that SpHMGB1‐B box peptide played an important role in mediating the immune response of S. prenanti.

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Ghrelin and NUCB2/Nesfatin‐1 Co‐Localization With Digestive Enzymes in the Intestine of Pejerrey (Odontesthes bonariensis)

Bertucci

Blanco

Sánchez-Bretaño

et al. 2018

The Anatomical Record

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Ghrelin (orexigenic) and nesfatin‐1 (anorexigenic) are two peptides with opposing actions on food intake regulation and are mainly expressed in the hypothalamus and gut of mammals and fish. Both are involved in the regulation of a wide range of physiological processes in vertebrates, including metabolism, growth, and reproduction. However, the anatomical relationship between these peptides and the nutrient assimilation processes are not well understood. Thus, the aim of this work was to determine the localization of ghrelin, nesfatin‐1, and several enzymes involved in the digestive process (lipoprotein lipase, aminopeptidase A, trypsin, and sucrase‐isomaltase) in the intestine of pejerrey (Odontesthes bonariensis), a species with commercial importance in South America. We observed co‐localization of ghrelin and nesfatin‐1 in enteroendocrine cells, absorptive cells, and in cells of the lamina propia. Approximately half of the cells displaying ghrelin‐like immunoreactivity co‐localized the NUCB2/nesfatin‐1‐like signal. In addition, both peptides showed co‐localization with lipoprotein lipase, aminopeptidase A, trypsin, or sucrase‐isomaltase. All digestive enzymes except for aminopeptidase A and trypsin, showed high co‐localization (68–88%) with both ghrelin‐like and NUCB2/nesfatin‐1‐like signals in absorptive, enteroendocrine, and lamina propria cells. Together, our results provide immunohistochemical evidence supporting a role for both ghrelin and NUCB2/nesfatin‐1 in the regulation of nutrient assimilation in fish. Anat Rec, 302:973–982, 2019. © 2018 Wiley Periodicals, Inc.

show abstract

Molecular characterization, tissue distribution and feeding related changes of NUCB2A/nesfatin-1 in Ya-fish (Schizothorax prenanti)

Cited by 49 publications

References 51 publications

Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain

Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain

Expression profiling and inflammatory activation analysis of high‐mobility group box 1 in Schizothorax prenanti

Ghrelin and NUCB2/Nesfatin‐1 Co‐Localization With Digestive Enzymes in the Intestine of Pejerrey (Odontesthes bonariensis)

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