Ghrelin and NUCB2/Nesfatin‐1 Co‐Localization With Digestive Enzymes in the Intestine of Pejerrey (Odontesthes bonariensis)

Abstract

Ghrelin (orexigenic) and nesfatin‐1 (anorexigenic) are two peptides with opposing actions on food intake regulation and are mainly expressed in the hypothalamus and gut of mammals and fish. Both are involved in the regulation of a wide range of physiological processes in vertebrates, including metabolism, growth, and reproduction. However, the anatomical relationship between these peptides and the nutrient assimilation processes are not well understood. Thus, the aim of this work was to determine the localization of ghrelin, nesfatin‐1, and several enzymes involved in the digestive process (lipoprotein lipase, aminopeptidase A, trypsin, and sucrase‐isomaltase) in the intestine of pejerrey (Odontesthes bonariensis), a species with commercial importance in South America. We observed co‐localization of ghrelin and nesfatin‐1 in enteroendocrine cells, absorptive cells, and in cells of the lamina propia. Approximately half of the cells displaying ghrelin‐like immunoreactivity co‐localized the NUCB2/nesfatin‐1‐like signal. In addition, both peptides showed co‐localization with lipoprotein lipase, aminopeptidase A, trypsin, or sucrase‐isomaltase. All digestive enzymes except for aminopeptidase A and trypsin, showed high co‐localization (68–88%) with both ghrelin‐like and NUCB2/nesfatin‐1‐like signals in absorptive, enteroendocrine, and lamina propria cells. Together, our results provide immunohistochemical evidence supporting a role for both ghrelin and NUCB2/nesfatin‐1 in the regulation of nutrient assimilation in fish. Anat Rec, 302:973–982, 2019. © 2018 Wiley Periodicals, Inc.

Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre‐juveniles after recruitment

“…Data are the mean ± activity was found in early and late pre-juveniles. This suggests that this activity could correspond to a sucrase-independent maltaseglucoamylase complex (Bertucci et al, 2019;Brun et al, 2020;Chaudet et al, 2019;Dhital et al, 2013;Morelos-Castro et al, 2020).…”

NUCB2/nesfatin-1 suppresses the acrosome reaction in sperm within the mouse epididymis

“…Nesfatin-1, a neuronal peptide responsible for regulating food intake and inducing satiety, is expressed in the hypothalamus and central nervous system (Goebel et al 2009 ; Goebel-Stengel et al 2011 ). However, recent studies revealed that NUCB2/nesfatin-1 is not only expressed in the hypothalamus, but also peripheral organs such as the pancreas, stomach, intestines, liver, kidneys, and adipose and reproductive tissues (Schalla and Stengel 2018 ; Bertucci et al 2019 ; Puzio et al 2021 ; Goyal and Dhar 2022 ). We have also demonstrated the expression of NUCB2/nesfatin-1 in the reproductive organs of mice, including ovaries, the uterus, and testes (Kim et al 2011 ; Kim et al 2014 ).…”

“…Recent reports demonstrated that nesfatin-1 is also expressed in peripheral organs of humans and rodents, and functions as a regulatory peptide (Dore et al 2017 ). In rats, nesfatin-1 immunoreactivity has been identified in the gastric tract, suggesting that it has a role in gastrointestinal function (Li et al 2013 ; Bertucci et al 2019 ). Nesfatin-1 is expressed in ghrelin-secreting X/A-like cells in the stomach, which stimulate appetite (Stengel et al 2010 ).…”

Ontogenetic Pattern Changes of Nucleobindin-2/Nesfatin-1 in the Brain and Intestinal Bulb of the Short Lived African Turquoise Killifish

“…Nesf-1 ir-cells were found in the feeding regulatory nuclei of the hypothalamus and anterior intestine of the goldfish [14] and in the mucosal cellular layer of the anterior gastrointestinal tract in zebrafish [16]. NUCB2/Nesf-1 and ghrelin co-localize in the enteroendocrine cells of the anterior intestine [17] and in the hepatopancreas of goldfish [18], in the gut mucosal cells of zebrafish [16], and in intestinal enteroendocrine cells of pejerry [19]. Furthermore, the anorexigenic role of Nesf-1 seems to also be conserved in goldfish and Ya-fish, where central or peripheral administration of Nesf-1 induces feeding behavior suppression [14,15,17] as well as in Siberian sturgeon, where it acts through the cholecystokinin signal pathway [20,21].…”