Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain

Hatef, Azadeh; Shajan, Sooraj; Unniappan, Suraj

doi:10.1016/j.ygcen.2014.09.009

Cited by 45 publications

(31 citation statements)

References 24 publications

(73 reference statements)

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“…NUCB2/nesfatin-1 enhances insulin release from β-cells and reduces food intake 11–14 . Our analysis revealed that with medium content of fat in the diet (MFD-C), significant lower nucb2a gene expression was observed compared to high (HFD-C) and low (LFD-C) content of fat in the diets (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

Falcinelli

Rodiles

Hatef

et al. 2017

Sci Rep

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In the present study, we explored whether dietary lipid content influences the gut microbiome in adult zebrafish. Diets containing three different lipid levels (high [HFD], medium [MFD], and low [LFD]) were administered with or without the supplementation of Lactobacillus rhamnosus (P) to zebrafish in order to explore how the dietary lipid content may influence the gut microbiome. Dietary lipid content shifted the gut microbiome structure. The addition of L. rhamnosus in the diets, induced transcriptional reduction of orexigenic genes, upregulation of anorexigenic genes, and transcriptional decrease of genes involved in cholesterol and triglyceride (TAG) metabolism, concomitantly with lower content of cholesterol and TAG. Probiotic feeding also decreased nesfatin-1 peptide in HFD-P and attenuated weight gain in HFD-P and MFD-P fed zebrafish, but not in LFD-P group. Intestinal ultrastructure was not affected by dietary fat level or probiotic inclusion. In conclusion, these findings underline the role of fat content in the diet in altering gut microbiota community by shifting phylotype composition and highlight the potential of probiotics to attenuate high-fat diet-related metabolic disorder.

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Section: Resultsmentioning

confidence: 99%

“…Immunofluorescence study was performed on 4 fish exactly as described previously 11, 57 . Briefly, paraffin-embedded sections (10 sections per fish) were deparaffinized in 100% xylene for 10 min and re-hydrated in graded ethanol series.…”

Section: Methodsmentioning

confidence: 99%

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

Falcinelli

Rodiles

Hatef

et al. 2017

Sci Rep

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“…In addition, NUCB2 mRNA levels increase in liver and hypothalamus in fish fed fat-enriched diets and decrease in gut after long-term feeding with a high-protein diet, suggesting that macronutrients regulate the expression of NUCB2/nesfatin-1 (Blanco et al, 2016a). In zebrafish, two isoforms of NUCB2 (NUCB2A and NUCB2B) exist, and both mRNAs decrease in the brain post-prandially and after food deprivation, suggesting an anorexigenic role for nesfatin-1 (Hatef et al, 2015). In Ya-fish, NUCB2A mRNA levels increase post-prandially in both hypothalamus and intestine, and fasting induces a decrease in NUCB2A mRNA levels in the hypothalamus, but an increase in the hepatopancreas, suggesting anorexigenic and metabolic roles (Lin et al, 2014b).…”

Section: Hormones Involved In Food Intakementioning

confidence: 99%

The Neuroendocrine Regulation of Food Intake in Fish: A Review of Current Knowledge

2016

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Fish are the most diversified group of vertebrates and, although progress has been made in the past years, only relatively few fish species have been examined to date, with regards to the endocrine regulation of feeding in fish. In fish, as in mammals, feeding behavior is ultimately regulated by central effectors within feeding centers of the brain, which receive and process information from endocrine signals from both brain and peripheral tissues. Although basic endocrine mechanisms regulating feeding appear to be conserved among vertebrates, major physiological differences between fish and mammals and the diversity of fish, in particular in regard to feeding habits, digestive tract anatomy and physiology, suggest the existence of fish- and species-specific regulating mechanisms. This review provides an overview of hormones known to regulate food intake in fish, emphasizing on major hormones and the main fish groups studied to date.

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“…Nesfatin-1 increases glucose-stimulated insulin secretion from b cells by direct action involving Ca 2+ influx through L-type calcium channels (Nakata et al, 2011). Nesfatin-1 was also identified in zebrafish (Hatef et al, 2014), Ya fish (Lin et al, 2014), trout (Caldwell et al, 2014) and frogs (Senejani et al, 2014). Nesfatin-1 suppresses feeding (Gonzalez et al, 2010;Kerbel and Unniappan, 2012), and reproductive hormone secretion in goldfish (Gonzalez et al, 2012b).…”

Section: Introductionmentioning

confidence: 99%

Nucleobindin-1 encodes a nesfatin-1-like peptide that stimulates insulin secretion

Ramesh

Mohan

Unniappan

2015

General and Comparative Endocrinology

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Nutrient status modulates the expression of nesfatin-1 encoding nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain

Cited by 45 publications

References 24 publications

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

Dietary lipid content reorganizes gut microbiota and probiotic L. rhamnosus attenuates obesity and enhances catabolic hormonal milieu in zebrafish

The Neuroendocrine Regulation of Food Intake in Fish: A Review of Current Knowledge

Nucleobindin-1 encodes a nesfatin-1-like peptide that stimulates insulin secretion

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