Molecular characterization of the genus Papaver section Oxytona using ISSR markers

Abstract

Papaver bracteatum Lindl., P. orientale L., and P. pseudo-orientale (Fedde) Medw., species of section Oxytona Bernh. in the genus Papaver L., are found widely in the native flora of Turkey. They are commercially important for their high alkaloid content. Because genus Papaver section Oxytona has similar morphological characters, it is not easy to distinguish these species. We used the inter-simple sequence repeat (ISSR) molecular marker system to determine the molecular characterisation of this section. In this study, 20 ISSR primers have been studied in 180 accessions collected from 5 different regions of Turkey. A total of 82 bands were obtained, of which 80 were polymorphic. Average genetic distance was found to be 0.35, the Shannon index was 0.50, and the polymorphism rate was 96.97%. The primer AT10 had the smallest number of bands, which was 3, while the primers AT19 and AT3 had the greatest number of bands, which was 5. In conclusion, the ISSR marker system can be used for the classification of section Oxytona.

Anticancer Activity of Papaver Somniferum

“…somniferum L. (the opium poppy) belongs to the family Papaveraceae and is widely used for medicinal purposes because it contains various alkaloids such as morphine, noscapine, narcotine, codeine, papaverine, and others [9][10][11]. Current studies show that alkaloids obtained from Papaver species could not only be used as analgesic or sedative but also in other areas such as cancer treatment [12].…”

When island-like populations at high elevation show genetic divergence despite no morphological variability: the case of Lupinus montanus in Central Mexico

“…How do patterns of genetic diversity and the structure of natural populations relate to past geological and climate events? In order to answer these questions we employed intersimple sequence repeat (ISSR) markers, which have been widely used to detect genetic diversity in plants (Ge et al, 2005a;Sica et al, 2005;Meloni et al, 2006;Alam et al, 2009;Escaravage et al, 2011;Gürkök et al, 2013). The ISSR technique presents several advantages: high reproducibility due to long primers that permit the use of stringent annealing temperatures (45-60 °C) and high polymorphism, including within a species (Pradeep Reddy et al, 2002).…”

New species and a synonym of the genus Hesperis (Brassicaceae) from Turkey

“…Our results are similar to those given in the literature (Figure 4). The ISSR marker system is a PCR-based technique that uses a single amplification primer composed of a microsatellite motif to target a subset of simple sequence repeats (SSRs) or microsatellites (Zietkiewicz et al, 1994;Gürkök et al, 2013). ISSR-PCR is a simple, quick, and efficient technique.…”