Molecular characterization of patchouli (Pogostemon spp) germplasm

Sandes, Silvio Santos; Zucchi, Maria Imaculada; Pinheiro, José Baldin; Bajay, Miklos Maximiliano; Batista, Carlos Eduardo de Araújo; Brito, Fred A.L.; Arrigoni-Blank, M.F.; Álvares-Carvalho, Sheila Valéria; Silva-Mann, Renata; Blank, A.F.

doi:10.4238/gmr.15017458

Cited by 10 publications

(9 citation statements)

References 28 publications

(17 reference statements)

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“…Thus, utmost care is necessary in order to avoid losing this accession, since it may be most important for future studies on genetic improvement. Ten patchouli accessions from the AGB of medicinal and aromatic plants of UFS were efficiently identified as two distinct genetic clusters by STRUCTURE (Sandes et al, 2016), highlighting the importance of such methods in studies with AGBs of medicinal and aromatic plants.…”

Section: Discussionmentioning

confidence: 99%

“…Because they are dominant loci, it is not possible to distinguish heterozygotes from homozygotes; however, multiple loci can be produced from each amplification with polymerase chain reaction (PCR; Goulão and Oliveira, 2001). Several genetic diversity studies on medicinal plants, both in natural populations and in collections of germplasm banks, have been carried out using ISSRs, such as studies on Capparis spinosa, Eucommia ulmoides, and Pogostemon cablin (Liu et al, 2015;Yu et al, 2015;Sandes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

et al. 2016

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ABSTRACT. Varronia curassavica Jacq. is a medicinal and aromatic plant from Brazil with significant economic importance. Studies on genetic diversity in active germplasm banks (AGB) are essential for conservation and breeding programs. The aim of this study was to analyze the genetic diversity of V. curassavica accessions of the AGB of Medicinal and Aromatic Plants of the Federal University of Sergipe (UFS), using inter-simple sequence repeat molecular markers. Twentyfour primers were tested, and 14 were polymorphic and informative, resulting in 149 bands with 97.98% polymorphism. The UPGMA dendrogram divided the accessions into Clusters I and II. Jaccard similarity coefficients for pair-wise comparisons of accessions ranged between 0.24 and 0.78. The pairs of accessions VCUR-001/VCUR-503, VCUR-001/VCUR-504, and VCUR-104/VCUR-501 showed relatively low similarity (0.24), and the pair of accessions VCUR-402/VCUR-403 showed medium similarity (0.78). Twenty-eight accessions were divided into three distinct clusters, according to the STRUCTURE analysis. The genetic diversity of V. curassavica in the AGB of UFS is low to medium, and it requires expansion. Accession VCUR-802 is the most suitable for selection in breeding program of this species, since it clearly represents all of the diversity present in the AGB.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

et al. 2016

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“…The reproducibility of this marker is associated with two characteristics that provide high specificity to the ISSR primers: high annealing temperature and design based on the microsatellite regions, which makes ISSR semi-arbitrary markers (Sandes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira¹,

Silva²,

Alves³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Brazil has about 300 Croton species in different types of vegetation. Croton tetradenius Baill., which is endemic to the Northeast region and predominant in the Caatinga vegetation, stands out among the several species of this genus. Considering the importance of knowing the genetic variability of a species, the objective of this study was to analyze the genetic diversity of the genotypes of natural populations of C. tetradenius in the State of Sergipe, using ISSR molecular markers. Forty individuals were sampled in four natural populations of the State of Sergipe, Brazil. Thirteen primers were used for DNA amplification using ISSR-PCR, totaling 77 amplified fragments, of which 94.8% were polymorphic. Results of the cluster analysis obtained by the Jaccard's similarity index, using the UPGMA method, resulted in the formation of six distinct clusters. Analysis of molecular variance (AMOVA), used to estimate the genetic variability among populations, revealed significant genetic variance (P < 0.01) between and within the 2 C.S. Almeida-Pereira et al.Genetics and Molecular Research 16 (2): gmr16029602 studied populations, and most of the genetic diversity was found (87%) within populations. According to the Jaccard's similarity index, none of the studied plants was genetically identical. CTE210 and CTE305 presented high similarity index (0.76), while CTE105 presented low similarity index (<0.16) with all related individuals. ISSR markers were efficient and allowed the formation of a molecular profile, and had sufficient polymorphism to estimate the genetic variability between the accessions of the studied populations.

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“…To determine genetic diversity, nuclear DNA were extracted fromPatchouli mutant young leafwith a modified CTAB method by Sandes et al (2016) by addition of MATAB 4%. PCR reaction were consisted with 3.0 μL 5 ng/μL DNA, 0.5 μL 10 μM forward primer, 0.5 μL 10 μM reverse primer, 1.6 μL 2.5 mM dNTPs, 1.0 μL 1 U/μL Taq DNA polymerase, 2.0 μL 10X PCR buffer (50 mM KCl, 10 mM Tris-HCl, pH 8.0), 0.8 μL 25 mM MgCl 2 , and 10.6 μL H 2 O.…”

Section: Methodsmentioning

confidence: 99%

“…The utilization of molecular markers on patchouli plants remains limited, while Sandes et al (2013) developed an SSR (Simple Sequence Repeat) markers to classify two Patchouli species. Examination on patchouli diversity with molecular markers were reported by Wu et al (2011) on genetic diversity through ISSR and SRAP markers, genome sequences on chloroplasts (He et al, 2016) and molecular characterization (Sandes et al, 2016). Utilization of molecular marker as a tool of selection in Patchouli is not widely known as reports concerning the selection of Patchouli in Indonesia remains insufficient.…”

Section: Abstract: Diversity Genotyping Patchouli Pogostemonmentioning

confidence: 99%

Genetic and Leaf Characteristic Diversity on 10 Mutant Progenies of Patchouli (Pogostemon cablin) Provide Insights to Selection Strategies

Tahir¹,

Riniarti²,

Ersan³

et al. 2019

Agrivita.J.Agr.Sci

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The importance of patchouli plants to produce essential oil has established this medicinal herb as one of the Indonesia's main economic contributor. To date, Indonesia can only provide 5 varieties i.e. Sidikalang, Tapak Tuan, Lhokseumawe, Patchoulina 1 and Patchoulina 2. In order to increase national patchouli production, several efforts needs to be done by providing new superior patchouli lines. This experiment was undertaken to reveal diversity of mutant progenies derived from gamma ray irradiation in patchouli. Six SSR primers were used to determine genetic diversity on MV3 generation of the mutants. Molecular analysis revealed a moderate to high polymorphism based on tested primers, which amplified 73 bands, and resulting of 0.705 PIC value. Dendrogram analysis based on Euclidean dissimilarity showed broad diversity that ranged from 0.23 to 0.82. Phenotypic tree gave clear separation that all mutant clones were clustered into three different group, with Eucledian dissimilarity coefficient ranging from 0.02 to 0.13. This report indicates that Patchouli vegetativelypropagated mutants were able to maintain their variability continuously from their parental lines (M0). Furthermore, our findings offer comprehensive information on breeding strategies of patchouli plants, giving an opportunity to assess important traits at early generation.

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Molecular characterization of patchouli (Pogostemon spp) germplasm

Cited by 10 publications

References 28 publications

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Genetic and Leaf Characteristic Diversity on 10 Mutant Progenies of Patchouli (Pogostemon cablin) Provide Insights to Selection Strategies

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