Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

Brito, Fred A.L.; Nízio, Daniela Aparecida Castro; Silva, A V C; Diniz, Leandro Eugênio Cardamone; Rabbani, Allívia Rouse Carregosa; Arrigoni-Blank, M.F.; Álvares-Carvalho, Sheila Valéria; Figueira, Glyn Mara; Júnior, Ílio Montanari; Blank, A.F.

doi:10.4238/gmr.15038681

Cited by 15 publications

(14 citation statements)

References 17 publications

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“…Many studies have proved the effectiveness of this marker in articles on genetic diversity and characterization of accessions between and within populations, such as those with Ocimum basilicum (Aghaei et al, 2012), Pitcairnia flammea (Souza-Sobreira et al, 2015), Croton heliotropiifolius (Rocha et al, 2016), Erythrina velutina (Souza et al, 2016), and Varronia curassavica (Brito et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira¹,

Silva²,

Alves³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Brazil has about 300 Croton species in different types of vegetation. Croton tetradenius Baill., which is endemic to the Northeast region and predominant in the Caatinga vegetation, stands out among the several species of this genus. Considering the importance of knowing the genetic variability of a species, the objective of this study was to analyze the genetic diversity of the genotypes of natural populations of C. tetradenius in the State of Sergipe, using ISSR molecular markers. Forty individuals were sampled in four natural populations of the State of Sergipe, Brazil. Thirteen primers were used for DNA amplification using ISSR-PCR, totaling 77 amplified fragments, of which 94.8% were polymorphic. Results of the cluster analysis obtained by the Jaccard's similarity index, using the UPGMA method, resulted in the formation of six distinct clusters. Analysis of molecular variance (AMOVA), used to estimate the genetic variability among populations, revealed significant genetic variance (P < 0.01) between and within the 2 C.S. Almeida-Pereira et al.Genetics and Molecular Research 16 (2): gmr16029602 studied populations, and most of the genetic diversity was found (87%) within populations. According to the Jaccard's similarity index, none of the studied plants was genetically identical. CTE210 and CTE305 presented high similarity index (0.76), while CTE105 presented low similarity index (<0.16) with all related individuals. ISSR markers were efficient and allowed the formation of a molecular profile, and had sufficient polymorphism to estimate the genetic variability between the accessions of the studied populations.

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Section: Discussionmentioning

confidence: 99%

“…(Brito et al, 2016), in Capparis spinosa L. (Liu et al, 2015), in Rheum (Tabin et al, 2016), in Withania somnifera (Khan and Shah, 2016), and in Panax stipuleanatus Tsai (Trieu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira¹,

Silva²,

Alves³

et al. 2017

Genet. Mol. Res.

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“…This is because the genetic content is little influenced by the environment and can provide useful information for the management of the species and the development of strategies for the conservation of genetic resources (Celestino et al, 2015;Baruah et al, 2017). Since it is an aromatic species with medicinal potential and found in areas of intense anthropic activity, it is necessary to obtain information on the genetic diversity of natural populations of E. fruticosa, which may guide subsequent stages of conservation and use of this species (Alves et al, 2016;Brito et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Research Article Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential.

et al. 2017

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ABSTRACT. Eplingiella fruticosa (Salzm. ex Benth.) Harley & J.F.B. Pastore, ex Hyptis fruticosa Salzm. ex Benth. is an aromatic and medicinal plant of the family Lamiaceae, found mainly in regions with intense anthropic activity. Information on the genetic diversity of this species is scarce. However, it can be assessed using molecular markers that identify the level of diversity among phenotypically identical individuals. The present study aimed to characterize the genetic diversity of a native population of E. fruticosa from the State of Sergipe using ISSR molecular markers. Samples of 100 plants were collected in 11 municipalities of the State of Sergipe and analyzed using eight ISSR primers, resulting in 72 informative bands. The cluster analysis obtained using the neighbor joining method resulted in three groups: Group I consisted of 50 plants, mainly from the municipalities of Areia Branca, Estância, Japaratuba, Moita Bonita, Pirambu, and Salgado; Group II was .250), and the greatest distance occurred between plants EPF50 and EPF96 (0.9778). The Shannon index had a mean value of 0.42, and diversity was considered moderate. Heterozygosity had a mean value of 0.267 and was considered low. Polymorphic information content (0.253) was considered moderately informative. Genetic diversity of E. fruticosa plants was intermediate, and the results of the present study can assist in the conservation and use of the genetic resources of this species.

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“…ISSR (inter-simple sequence repeat) molecular markers is an excellent tool for the genetic diversity analysis and characterization of accessions and cultivars of several species due to the high degree of polymorphism, reproducibility and low cost (Salazar-Laureles et al, 2015;Brito et al, 2016;Silva et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Desmanthus sp accessions using ISSR markers and morphological traits

Costa

Fracetto

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Desmanthus is a genus of forage legumes with high nutritional value, productive potential, and ability to obtain nitrogen in association with diazotrophic bacteria. The use of accurate techniques for genotype identification and characterization is essential for breeding programs. Morphological markers are widely used to know the genetic diversity and the molecular markers are fundamental in these studies. We investigated the genetic diversity among Desmanthus sp genotypes in Pernambuco (Brazilian Northeast State), using morphological traits and ISSR markers. Morphological and molecular characterizations were performed in 18 and 26 accessions, respectively, in plants belonging to the germplasm bank of forage legumes of Universidade Federal Rural de Pernambuco (UFRPE), Academic Unit of Serra Talhada, PE, Brazil. Eight ISSR primers were selected, and 95 loci were generated, with polymorphism of 95.79%. The allele number observed was 1.958, where the effective number was 1.359, and the Nei diversity genetic 2 J.C. Costa et al.Genetics and Molecular Research 16 (2): gmr16029667 index was 0.226. About the morphological markers, seed number was the variable that most contributed to the genetic divergence. A large amount of genetic diversity was observed among Desmanthus species, occurring spontaneously in Pernambuco, Brazil. Thus, the variability found in morphological and ISSR markers is importance for the improvement of Desmanthus spp. Our findings showed that 17L, 27L, 25F, 22F, 19S, 13Au, and 28G accessions could be used in breeding programs to explore the maximum genetic divergence.

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Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

Cited by 15 publications

References 17 publications

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Research Article Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential.

Genetic diversity of Desmanthus sp accessions using ISSR markers and morphological traits

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