Molecular characterization identifies intra‐host recombination and zoonotic potential of canine rotavirus among dogs from Thailand

Abstract

Rotavirus (RV) is an RNA virus belonging to the Reoviridae family.There are nine groups of rotaviruses (A-I). Group A rotavirus (RVA) is one of the major pathogens causing gastroenteritis in humans and animals worldwide (Greenberg & Estes, 2009). The virus contains 11 dsRNA segments encoding viral structure proteins (VP1, VP2, VP3, VP4, VP6 and VP7) and non-structural proteins (NSP1, NSP2, NSP3, NSP4, NSP5 and NSP6). The RVAs can be classified based on two classification systems. In the first classification system, two outer layer proteins (VP7 and VP4) are used to determine the genotype by G and [P]. There are 35G (G1-G35) and 50P (P[1]-P[50])

Molecular evolution and genetic characteristics of G3P[3] group A canine rotavirus isolated in Wuhan, China

“…Based on the phylogenetic tree analysis of the whole genome of the isolated strain, the nucleotide sequences of some of the gene fragments were highly homologous to other species such as the VP7, VP6, NSP1 and NSP3 genes. The VP7 and VP4 genes of the isolated strain in this study belonged to the G3 genotype (AU-1-like) and the P [3] genotype (Cat 97-like), which are the most common genotypes in dogs. The phylogenetic analysis assay (Fig.…”

“…The G3P [3] genotype is the predominant genotype in dogs and the isolated strain belonged to this group. Most gene sequences were closely related to the Thai group A CRVs (CU128, CU132 and CU20139).…”

“…Most gene sequences were closely related to the Thai group A CRVs (CU128, CU132 and CU20139). These Thai group A CRVs were first reported in the Thai kennel industry in 2021, and may have originated from human and bat group A rotavirus through multiple reassortments [3]. We suspect that the isolated strain and Thai strains may have a closely related origin, and that the strain may also have been derived from human and bat group A rotavirus, or spread from Thailand to Wuhan.…”

“…Isolation and characterization of RVA/Canine-tc/CHn/WH/2020/G3P [3] In this study, CRV was isolated from a 2-month-old Yorkshire dog that was also infected with CPV. The clinical symptoms of this dog included haematochezia.…”

“…The CDS of the VP1 gene of the isolated strain RVA/Canine-tc/CHN/WH/2020 /G3P [3] was amplified by RT-PCR. The full-length CDS was 3267 bp, which encoded a total of 1 088 amino acids.…”