A G3P[9] rotavirus strain with an unusual genome constellation in a diarrheic cat in Thailand

Lestari, Fajar Budi; Chandranoi, Kirkvich; Chuchaona, Watchaporn; Vongpunsawad, Sompong; Poovorawan, Yong

doi:10.1007/s00705-022-05641-1

Cited by 9 publications

(7 citation statements)

References 12 publications

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“…26 However, a similar strain (G3-P[9]-I2-R2-C2-M2-A3-N2-T3-E3-H3) was recently identified in 2021 in Thailand, and was originated from a feline with diarrhoea. 27 The rare combination of G3-P[9]-I2-E3 that was detected in this study is possibly derived from a reassortment event. Reassortment is common among RVs and is a crucial mechanism for the evolution of the virus.…”

Section: Discussionmentioning

confidence: 68%

Molecular characterization of VP6 and NSP4 genes of unusual G and P Rotavirus group A isolated from children with acute gastroenteritis

Tatsi,

Dellis,

Koukou

et al. 2023

Preprint

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Group A Rotavirus (RVA), which causes acute gastroenteritis (AGE) in children worldwide, is categorized mainly based on VP7 (genotype G) and VP4 (genotype P) genes. Genotypes that circulate at <1% are considered unusual. Important genes are also VP6 (genotype I) and NSP4 (genotype E). VP6 establishes the group and affects immunogenicity, while NSP4, as enterotoxin, is responsible for the clinical symptoms. Aim of this study was to genotype and molecularly characterize the VP6 and NSP4 genes of unusual RVA. Unusual RVA strains isolated from fecal samples of children ≤16 years with AGE, were genotyped in VP6 and NSP4 genes with Sanger sequencing. Phylogenetics was performed using the MEGA 11 program. In a 15-year period (2007-2021), 54.8% (34/62) of unusual RVA were successfully I and E genotyped. Three different I and E genotypes were identified; I2 (73.5%, 25/34) and E2 (35.3%, 12/34) were the commonest. E3 genotype was detected from 2017 onwards. The uncommon combination of I2-E3 was found in 26.5% (9/34) of the strains and G3-P[9]-I2-E3 was the most frequent G-P-I-E combination (20.6%, 7/34). Statistical analysis showed that children infected with E2 strains had a higher relative frequency of dehydration (50%) compared to those with the E3 genotype ( p=0.019). Multiple substitutions were detected in both genes, but their functional effect remains unknown. The results of this study highlight the genetic diversity of RVA strains. Continuous surveillance of the RVA based on the whole genome will provide a better knowledge of its evolution.

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Section: Discussionmentioning

confidence: 68%

Molecular characterization of VP6 and NSP4 genes of unusual G and P Rotavirus group A isolated from children with acute gastroenteritis

Tatsi,

Dellis,

Koukou

et al. 2023

Preprint

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“…No gene segments of the representative G3P[9] feline/human‐like (FRV‐1/AU‐1‐like) or G3P[3] feline/canine‐like (Cat97/CU‐1‐like) strains shared > 98% nucleotide identity with those of Mie20120017f (Table 1). Among other feline strains, G3P[9] strain THA/Meesuk/2021 most closely resembled Mie20120017f, sharing > 95% nucleotide identity across seven genetic segments (VP4, VP1, and NSP1–5) 32 …”

Section: Resultsmentioning

confidence: 99%

“…THA/Meesuk/2021 most closely resembled Mie20120017f, sharing > 95% nucleotide identity across seven genetic segments (VP4, VP1, and NSP1-5). 32 The VP7 (G6) gene of Mie20120017f shared high nucleotide identity (97.6%-98.0%) and amino acid identity (98.4%-99.5%) with G6P [8] strains identified in Brazil between 2019 and 2022, such as LVCA_30388, AM668, and LVCA_33925 (Table 1). 33 human-like, FRV-1/AU-1-like).…”

Section: Mie20120017f (G6p[9])mentioning

confidence: 99%

Human transmission and outbreaks of feline‐like G6 rotavirus revealed with whole‐genome analysis of G6P[9] feline rotavirus

Fukuda,

Kusuhara,

Takai‐Todaka

et al. 2024

Journal of Medical Virology

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Group A rotaviruses (RVAs) are generally highly species‐specific; however, some strains infect across species. Feline RVAs sporadically infect humans, causing gastroenteritis. In 2012 and 2013, rectal swab samples were collected from 61 asymptomatic shelter cats at a public health center in Mie Prefecture, Japan, to investigate the presence of RVA and any association with human infections. The analysis identified G6P[9] strains in three cats and G3P[9] strains in two cats, although no feline RVA sequence data were available for the former. A whole‐genome analysis of these G6P[9] strains identified the genotype constellation G6‐P[9]‐I2‐R2‐C2‐M2‐A3‐N2‐T3‐E3‐H3. The nucleotide identity among these G6P[9] strains exceeded 99.5% across all 11 gene segments, indicating the circulation of this G6P[9] strain among cats. Notably, strain RVA/Human‐wt/JPN/KF17/2010/G6P[9], previously detected in a 3‐year‐old child with gastroenteritis, shares high nucleotide identity (>98%) with Mie20120017f, the representative G6P[9] strain in this study, across all 11 gene segments, confirming feline RVA infection and symptomatic presentation in this child. The VP7 gene of strain Mie20120017f also shares high nucleotide identity with other sporadically reported G6 RVA strains in humans. This suggests that feline‐origin G6 strains as the probable source of these sporadic G6 RVA strains causing gastroenteritis in humans globally. Moreover, a feline‐like human G6P[8] strain circulating in Brazil in 2022 was identified, emphasizing the importance of ongoing surveillance to monitor potential global human outbreaks of RVA.

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“…Rotaviruses can be transmitted as interspecies as a result of genetic reassortment and this situation may result in genetic similarity between rotaviruses that infect different species. There are some examples for interspecies transmission of RVA from bovine to heterologous species, such as from bovine to rabbit (K1130027 strain), from bovine to horse (OH-4 strain) [35,36], and with some recent data, from bovine to feline transmission was shown in other studies [37,38]. It was previously shown that the NSP5 gene of feline RVA strain shares high nucleotide identity with BRV (97%) and it was suggested to have been acquired from a bovine rotavirus through reassortment [38].…”

Section: Declarations Conflict Of Interestmentioning

confidence: 97%

“…In a recent study, the R11-035 strain of a rotavirus, which was transferred from a cat to a human, acquired NSP3 through intergenotype reassortment with strains found in pigs before being transmitted [17]. Phylogenetic analysis of a recent study revealed that some of the genes of the rotavirus (VP4, VP7, NSP1, NSP3, NSP4, and NSP5) were similar to those found in human feline-like rotaviruses, while other genes (VP1, VP2, VP3, VP6, and NSP2) were more similar to those found in human bovine-like rotaviruses [37] The results of this study are consistent with previously published ndings in the area. These results demonstrated that rotaviruses have international, intercontinental and interspecies transmission and Turkish bovine RVA eld isolate can be the source of reassortment that culminates with interspecies transmission.…”

Section: Declarations Conflict Of Interestmentioning

confidence: 99%

Genome characterization of a Turkish bovine rotavirus field isolate by shotgun metagenomics

Aksoy

Azkur

2023

Arch Virol

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Bovine rotavirus (BRV) which is the leading viral agent of neonatal calf diarrhea. In recent years, the use of shotgun metagenomics has emerged as a powerful tool for the detection and characterization of viruses. In the present study, BRV was isolated on MA-104 cells from feces of a neonatal calf with diarrhea and shotgun metagenomics was performed in order to obtain whole genome of BRV isolate. From nine sequenced segments, ve segments encoding VP1, VP6, NSP3, NSP4, and NSP5/6 proteins was sequenced in full-length and four segments encoding the VP2, VP3, VP7, and NSP2 proteins was sequenced partially and are deposited in GenBank. Sequences of 9 out of 11 segments of BRV can be gained from metagenomics and the BRV isolate which is named as "RVA/Cow-tc/TUR/KIRSEHIR/2019/G10P[X]" is genotyped as G10-P[X]-I2-R2-C2-M2-AX-N2-T6-E2-H3. The VP1 and VP7 nucleotide and amino acid sequences of the present virus are conserved among Turkish BRV strains. Moreover, the VP2 gene/protein was genetically close to a number of RVA strains of cat and dog. Interestingly, for the NSP4 gene/protein, many equine rotaviruses from Japan and feline rotaviruses were found to be similar to KIRSEHIR strain. Although the highest nucleotide identity for NSP5/6 gene was with buffalo rotaviruses, amino acid identity and similarity were highest with human rotaviruses. Additionally, the study found that the present virus had some variations in its amino acid sequences and ndings from amino acid sequence identity and similarity are almost compatible with nucleotide sequences. Our results suggested that the present bovine RVA eld isolate might be genetic ancestor of canine, equine and feline rotaviruses and support the ndings of previous studies about interspecies transmission.

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A G3P[9] rotavirus strain with an unusual genome constellation in a diarrheic cat in Thailand

Cited by 9 publications

References 12 publications

Molecular characterization of VP6 and NSP4 genes of unusual G and P Rotavirus group A isolated from children with acute gastroenteritis

Molecular characterization of VP6 and NSP4 genes of unusual G and P Rotavirus group A isolated from children with acute gastroenteritis

Human transmission and outbreaks of feline‐like G6 rotavirus revealed with whole‐genome analysis of G6P[9] feline rotavirus

Genome characterization of a Turkish bovine rotavirus field isolate by shotgun metagenomics

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