Full genotype characterization of Brazilian canine G3P[3] strains during a 10-year survey (2012–2021) of rotavirus infection in domestic dogs and cats

Azevedo, Laís Sampaio de; Costa, Fernanda Faria; Ghani, Mohamed Kamel Abdul; Viana, Ellen; França, Yasmin; Medeiros, Roberta Salzone; Guiducci, Raquel; Morillo, Simone Guadagnucci; Primo, Dieli; Lopes, Ricardo Duarte; Gomes‐Gouvêa, Michele Soares; Costa, Antônio Charlys da; Luchs, Adriana

doi:10.1007/s00705-023-05807-5

Cited by 3 publications

(3 citation statements)

References 70 publications

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“…The 438 dog samples from Wuhan in China during 2019-2021 had a positivity rate of 1.60% (7/438) for species A CRV [12]. Between 2012 and 2021, 516 fecal samples from dogs in Brazil showed a positivity rate of 0.6% (3/516) for species A CRV [13]. From the 1990s to 2020, the CPV-2 morbidity and mortality rates in China varied from 3.90% to 95.8% and from 20.17% to 73.47%, respectively [28].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, CPV shows high genetic variation, and variants of CPV can cause serious harm to dogs [9,10]; some variants of CPV can even infect felines besides canines [11]. The G3 group CRV's inter-species transmission to children has been reported in Italy and China [12,13], and its zoonotic potential is of special interest to public health. In addition, co-infection with these viruses exacerbates clinical signs and pathological damage to infected dogs [5,14,15].…”

Section: Introductionmentioning

confidence: 99%

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A Quadruplex Reverse Transcription Quantitative Polymerase Chain Reaction for Detecting Canine Coronavirus, Canine Rotavirus, Canine Parvovirus, and Canine Distemper Virus

Shi,

Long,

Shi

et al. 2024

Microbiology Research

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Background: Canine coronavirus (CCoV), canine rotavirus (CRV), canine parvovirus (CPV), and canine distemper virus (CDV) cause gastroenteritis in dogs, and co-infections of these pathogens are common in China. In particular, CCoV and CRV are confirmed to have important zoonotic potential and cause public health issues. It is difficult to diagnose these diseases based only on clinical manifestations and pathological damage. Methods: In this study, four pairs of specific primers and probes targeting the CCoV M, CRV VP7, CPV VP2, and CDV N genes were designed. The reaction conditions, including the primer and probe concentrations, annealing temperatures, and reaction cycles, were optimized for the development of a quadruplex RT-qPCR for the detection of CCoV, CRV, CPV, and CDV. The assay was used to test 1028 clinical samples to validate its application. Results: A quadruplex RT-qPCR was successfully established for the differential detection of CCoV, CRV, CPV, and CDV, with good specificity, high sensitivity, and excellent repeatability. The assay could specifically detect CCoV, CRV, CPV, and CDV without cross-reactivity with the other canine viruses tested. It showed high sensitivity with limits of detection (LOD) of 1.1 × 102 copies/reaction for all four plasmid constructs. It showed excellent repeatability, with 0.05–0.90% intra-assay variation and 0.02–0.94% inter-assay variation. The 1028 clinical samples were tested using the quadruplex RT-qPCR and a reported reference RT-qPCR. The positivity rates of CCoV, CRV, CPV, and CDV were 9.53%, 0.97%, 25.68%, and 5.06% using the developed assay, and 9.05%, 0.88%, 25.68%, and 4.86% using the reference assay, with agreements higher than 99.32%. Conclusion: The results indicated that a rapid and accurate quadruplex RT-qPCR was developed for the detection and differentiation of CCoV, CRV, CPV, and CDV.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Quadruplex Reverse Transcription Quantitative Polymerase Chain Reaction for Detecting Canine Coronavirus, Canine Rotavirus, Canine Parvovirus, and Canine Distemper Virus

Shi,

Long,

Shi

et al. 2024

Microbiology Research

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“…Rotavirus (RV) belongs to the family Reoviridae and wheel-shaped, triple-layered virion with a diameter of about 100 nm (Nirmal and Gangar 2023). They have an 11-segment genome that codes for 5 nonstructural proteins (NSP1, NSP2, NSP3, NSP4, and NSP5) and 6 structural viral proteins (VP1, VP2, VP3, VP4, VP6 and VP7) (Azevedo et al 2023). RV strains are categorized based on the differences between two outside proteins on the virus surface known as VP4 (P-type) and VP7 (G-type) (McDonald et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Control Strategies of Rotavirus Infection

2023

IJAB

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Rotavirus is major cause of gastroenteritis particularly in young and newborn children. The main way that the virus spreads is by the fecal-oral route, however, contaminated food, drink, and surfaces can also pose a significant risk of transmission. Due to inadequate sanitation and medical facilities, low-and middle-income nations are disproportionately affected by RV infections, which cause severe morbidity and mortality on a global scale. Various animals are affected by RV infections in addition to people, resulting in a variety of types. Infection of host cells, virus replication, assembly, and release of new virus particles are all phases of the RV life cycle. Personal contact, contaminated objects, and airborne routes are the three ways the disease spreads. According to epidemiology, childhood RV infections are common, vary seasonally, and are more severe in low-income countries. RV vaccinations, such as RotaTeq and Rotarix have successfully avoided severe gastroenteritis. Passive immunization is the main focus of animal vaccines; however, RV Virus-like Particles (RV-VLPs) promise to be a more broadly serotype-covered vaccine in the future. RV continues to be a major public health concern, and developments like RV-VLPs, as well as the development and execution of efficient immunization programs, are essential for the prevention and management of disease worldwide.

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Genetic diversity and evolution of G12P[6] DS-1-like and G12P[9] AU-1-like Rotavirus strains in Brazil

França,

Medeiros,

Viana

et al. 2024

Funct Integr Genomics

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Full genotype characterization of Brazilian canine G3P[3] strains during a 10-year survey (2012–2021) of rotavirus infection in domestic dogs and cats

Cited by 3 publications

References 70 publications

A Quadruplex Reverse Transcription Quantitative Polymerase Chain Reaction for Detecting Canine Coronavirus, Canine Rotavirus, Canine Parvovirus, and Canine Distemper Virus

A Quadruplex Reverse Transcription Quantitative Polymerase Chain Reaction for Detecting Canine Coronavirus, Canine Rotavirus, Canine Parvovirus, and Canine Distemper Virus

Control Strategies of Rotavirus Infection

Genetic diversity and evolution of G12P[6] DS-1-like and G12P[9] AU-1-like Rotavirus strains in Brazil

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