Molecular and Antigenic Comparison of
            <i>Ehrlichia canis</i>
            Isolates from Dogs, Ticks, and a Human in Venezuela

Ünver, Ahmet; Perez, Miriam; Orellana, Nelson G.; Huang, Haibin; Rikihisa, Yasuko

doi:10.1128/jcm.39.8.2788-2793.2001

Cited by 95 publications

(87 citation statements)

References 24 publications

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“…There are scarce data available regarding E. canis infection in R. sanguineus (MURPHY et al, 1998;UNVER et al, 2001;NDIP et al, 2007). In the study performed by Aguiar et al (2007b) in Monte Negro (State of Rondônia) and São Paulo (State of São Paulo), E. canis-infection rates in R. sanguineus ticks varied from 2.4 (4/165) to 6.2% (10/162).…”

Section: Discussionmentioning

confidence: 96%

“…A new subspecies of Ehrlichia canis, recently characterized in Venezuela, underscored the zoonotic potential of this genre, in view of identifying persistent and asymptomatic infection in a human patient with involvement of a dog and also ticks infected by the same microorganism (PEREZ; RIKIHISA; WEN, 1996;PEREZ et al, 2006;UNVER et al, 2001). DNA of E. chaffeensis has also been detected in members of the Cervidae family (Blastocerus dichotomus) captured in the area of the Paraná River in southeastern São Paulo and eastern Mato Grosso do Sul, Brazil (MACHADO et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of ehrlichial infection among dogs and ticks in Northeastern Brazil

Souza¹,

Leal²,

Barboza³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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This study investigated the epidemiology of canine ehrlichiosis in Northeastern Brazil, focusing the identification of the Ehrlichia species and vectors involved. Samples were collected from 472 domestic dogs residing in the health districts of Cajazeiras and Itapuã of Salvador city. The average prevalence of antibodies reactive to E. canis by immunofluorescent antibody test (IFAT) (titer ≥ 1:80) was 35.6% (168/472). Blood samples from the E. canis-seropositive animals were tested by nested PCR in order to identify the Ehrlichia species responsible for the infection. Among the seropositives, 58 (34.5%) were found to be PCR-positive for E. canis. Ticks were found in 32 dogs. Nested-PCR analysis showed that 21.9% (7/32) of the Rhipicephalus sanguineus were infected by E. canis. In both dogs and Rhipicephalus sanguineus, nested-PCR for E. ewingii and E. chaffeensis was negative, with no amplification of DNA fragment.Keywords: Ehrlichia, PCR, epidemiology. ResumoEste estudo objetivou pesquisar a epidemiologia da erliquiose canina no Nordeste do Brasil, com especial atenção na identificação da espécie de Ehrlichia envolvida nas infecções caninas e vetoriais detectadas. Para isso foram coletadas amostras de 472 cães domiciliados nos distritos sanitários de Cajazeiras e Itapuã. A prevalência de anticorpos anti-E. canis, pela imunofluorescência indireta (título ≥ 1:80), em cães foi de 35,6% (168/472). Os animais soropositivos foram analisados por uma nested-PCR para identificação da espécie de Ehrlichia responsável pela infecção. Dentre os positivos, 58 (34,5%) cães foram PCR-positivos para E. canis. Foram coletados e classificados os carrapatos em 32 cães. A nested-PCR de Rhipicephalus sanguineus resultou em 21,9% (7/32) de infecção por E. canis. A nested-PCR de amostras de sangue de cães e Rhipicephalus sanguineus para E. chaffeensis e E. ewingii foi negativa, não havendo amplificação de fragmento de DNA.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Prevalence of ehrlichial infection among dogs and ticks in Northeastern Brazil

Souza¹,

Leal²,

Barboza³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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“…Canine monocytic ehrlichiosis (CME) is a major infectious disease that affects dogs and is also of interest in public health due to the recent discoveries of infection by E. canis in humans in Venezuela, where the agent was likely transmitted by the tick vector R. sanguineus (48,49) . Thus, taking into consideration the concern about the zoonotic potential of E. canis and other hemoparasites, further studies on the parasitism of humans by the R. sanguineus are necessary.…”

Section: Resultsmentioning

confidence: 99%

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

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The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI 95 17.7% -30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI 95 40.0% -59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P>0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P<0.05). However, for the erythrogram, the platelet count, the leukogram and clinical signs, no significant difference (P>0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis. Keywords: babesiosis; canine; ehrlichiosis; IFAT; laboratory findings. ResumoO objetivo deste estudo foi determinar a prevalência de anticorpos IgG anti-Ehrlichia canis e antiBabesia vogeli em cães e correlacionar com a prevalência e fatores de risco para avaliar a relação do estado sorológico com os achados hematológicos. Amostras de sangue de cães coletadas de setembro de 2011 a março de 2012, no Hospital Veterinário da Universidade Federal de Lavras, Brasil, foram analisadas usando o teste de reação de imunofluorescência indireta (RIFI). Do total de amostras de soro de 160 cães, 23,7% (38 cães; IC95 17,7% -30,7%) foram soropositivos para E. canis, 40,0% (64 cães; IC95 40,0% -59,2%) para B. vogeli, e 5,6% (9 cães) para os dois hemoparasitas. Nenhuma das variáveis epidemiológicas mostrou associação significativa (P> 0,05) com a soropositividade para E. canis e B. vogeli. Cães soropositivos para E. canis mostraram valores médios mais baixos para hematócrito (P<0,05). No entanto, para o eritrograma, a contagem de plaquetas, o leucograma e os sinais clínicos, nenhuma diferença significativa (P> 0,05) foi observada entre cães soropositivos e soronegativos para E. canis ou para B. vogeli. Os resultados sorológicos deste estudo sugerem que a Cienc. anim. bras., Goiânia, v.18, 1-9, e-36095, 2017 2 FONSECA, J.P., et al.infecção por E. canis e B. vogeli é endêmica na população canina em questão, com uma prevalência da fase subclínica (assintomática) em cães soropositivos para erliquiose ou babesiose.

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“…Antibodies to E. canis have also been detected in almost all Brazilian states, with the prevalence of exposure in dogs ranging from 4.8% (SAITO et al, 2008) to 73% (NAKAGHI et al, 2008). Although E. canis DNA has been detected in humans in Venezuela (PEREZ et al, 2006;UNVER et al, 2001), the importance of the parasite as a zoonotic agent remains unknown.…”

Section: Igg Antibodies Tomentioning

confidence: 99%

Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil

Sousa

André

Herrera³

et al. 2013

Rev. Bras. Parasitol. Vet.

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Tick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detect Ehrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis of L. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies to Ehrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, for E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys and Leishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis.Keywords: Ehrlichia canis, Babesia vogeli, Anaplasma platys, Leishmania infantum, dogs, co-infection. ResumoPatógenos transmitidos por carrapatos atingem uma variedade de hospedeiros vertebrados. Para identificar os agentes patogênicos transmitidos por carrapatos entre cães soropositivos para Leishmania infantum no município Campo Grande-MS, foi realizado um estudo sorológico e molecular para a detecção de Ehrlichia canis, Anaplasma platys e Babesia vogeli em 60 amostras de soro e baço, respectivamente. Adicionalmente, foi realizado o diagnóstico confirmatório de L. infantum por meio de técnicas sorológicas e moleculares. Também foi realizado o alinhamento e análise filogenética das sequências para indicar a identidade das espécies de parasitas que infectam esses animais. Anticorpos IgG anti-Ehrlichia spp., anti-B. vogeli e anti-L. infantum foram detectados em 39 (65%), 49 (81,6%) e 60 (100%) dos cães amostrados, respectivamente. Vinte e sete (45%), cinquenta e quatro (90%), cinquenta e três (88,3%), dois (3,3%) e um (1,6%) cães mostraram-se positivos na PCR para E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. e Anaplasma sp., respectivamente. Após o seqüenciamento, os amplicons mostraram 99% de similaridade com isolados de E. canis, B. vogeli e A. platys e Leishmania chagasi. Os resultados deste estudo indicaram que os cães soropositivos para L. infantum de Campo Grande, MS, são expostos a vários agentes transmitidos por carrapatos, e, portanto, devem ser incluídos no diagnóstico diferencial em cães com suspeita clínica de leishmaniose.

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Molecular and Antigenic Comparison of Ehrlichia canis Isolates from Dogs, Ticks, and a Human in Venezuela

Cited by 95 publications

References 24 publications

Prevalence of ehrlichial infection among dogs and ticks in Northeastern Brazil

Prevalence of ehrlichial infection among dogs and ticks in Northeastern Brazil

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil

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