Modulation in arsenic-induced lipid catabolism in Glycine max using proline, 24-epibrassinolide and diphenylene iodonium

Chandrakar, Vibhuti; Parkhey, Suruchi; Dubey, Amit; Keshavkant, S.

doi:10.1515/biolog-2017-0033

Cited by 20 publications

(12 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accrual of As inside the cells might be the result of damaged cell membranes, alterations in the cell wall structures and/ or rapid influx of it through transporters of the exposed cells (Singh et al 2015). A similar result was recently recorded by Chandrakar et al (2017b). On the other hand, exogenous application of DPI, EBL or Pro with Fig.…”

Section: Discussionsupporting

confidence: 76%

“…The detrimental impacts of As in Glycine max L., however, was attenuated substantially after the addition of DPI, EBL or Pro; moreover, among the three treatments, Pro was recognized to be the most effective against As-stress. Since Pro acts as a free radical scavenger and membrane stabilizer, it protects the lipid fractions from being oxidized, and thereby there is less accumulation of lipid peroxidized products (Siddiqui et al 2015, Chandrakar et al 2017b. Arsenic-prompted increase in ROS accumulation led oxidation of proteins and was analyzed by measuring protein and its oxidized products (PCO and PrOOH)/ aldehydic adducts (MDA-and HNE-protein adducts).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, protein hydroperoxides (PrOOHs), the most abundant and reactive molecules, are also produced as a derivative of ROS-mediated oxidation of proteins, and are shown to contribute to PCO turnover (Mallick et al 2013). Moreover, in stressed cells, proteins are also shown to combine readily with lipid peroxidized products such as malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), thus forming cytotoxic compounds MDA-/HNE-protein adducts, which are genotoxic in nature and are resistant to proteases (Parkhey et al 2014a, Chandrakar et al 2017b. Proteasomes are shown to be involved actively in elimination of degraded, aggregated and/ or misfolded proteins (Karmous et al 2014).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Modulation of arsenic-induced oxidative stress and protein metabolism by diphenyleneiodonium, 24-epibrassinolide and proline in Glycine max L.

Chandrakar¹,

Dubey²,

Keshavkant³

2018

Acta Botanica Croatica

Self Cite

View full text Add to dashboard Cite

Arsenic (As)-toxicity is a major constraint for crop production. The present study was intended to examine the comparative ameliorative effects of diphenyleneiodonium (DPI), 24-epibrassinolide (EBL) and proline (Pro) on As-stress inGlycine maxL. Seeds ofGlycine maxL. were subjected to As (100 µM) singly, and together with DPI (10 µM), EBL (0.5 µM) or Pro (10 mM), for five days, and were then analyzed. Experimental results showed that As treatment caused a substantial fall in growth traits like germination percentage, radicle length and dry mass, which was accompanied by As accumulation. Additionally, As application also revealed reduced viability, total protein content and activities of antioxidative enzymes (superoxide dismutase, catalase and ascorbate peroxidase), while it increased the levels of total sugar, proline and oxidative stress markers such as electrolyte leakage, reactive oxygen species, lipid oxidized products, protein carbonyls and hydroperoxides, Amadori and Maillard reaction products, malondialdehyde-/4-hydroxy-2-nonenal-protein adducts, protease and proteasome. Isozymes of antioxidative enzymes were also observed to be altered considerably under As-stress. Impressively, DPI, EBL and Pro played their role as protective agents, hence caused enhanced growth and reduced As accumulation. These protective chemicals also improved the viability, accruals of total protein, total sugar and endogenous proline, and activities of antioxidants, while they reduced the levels of oxidative stress markers. Our findings demonstrated the involvement of DPI, EBL and Pro in As-stress tolerance inGlycine maxL. Further, Pro appears to be superior to DPI and EBL, in alleviating As-induced responses inGlycine maxL.

show abstract

Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Modulation of arsenic-induced oxidative stress and protein metabolism by diphenyleneiodonium, 24-epibrassinolide and proline in Glycine max L.

Chandrakar¹,

Dubey²,

Keshavkant³

2018

Acta Botanica Croatica

Self Cite

View full text Add to dashboard Cite

show abstract

“…Membrane lipids are also deteriorated enzymatically by LOX, and activity of this enzyme was increased on exposure to As (Table ), which is incongruent with the findings of Chandrakar et al . However, the addition of NO or DMTU into As solution, more effectively NO, allowed the lower activity of LOX.…”

Section: Discussionmentioning

confidence: 56%

“…Glycine max L. (soybean) is an important crop, a potent source of both oil and protein, and is sensitive to As contamination . Based on the functions of NO and DMTU in plants exposed to various stressful environments, in the present study the authors have addressed the question of whether exogenous applications of each of NO and DMTU can enhance the adaptation of G. max L. to As stress.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide and dimethylthiourea up‐regulates pyrroline‐5‐carboxylate synthetase expression to improve arsenic tolerance in Glycine max L.

Chandrakar

Keshavkant

2018

Env Prog and Sustain Energy

Self Cite

View full text Add to dashboard Cite

Arsenic (As) is a toxic metalloid, severely affecting the normal functioning of plants, animals, and human beings. Nitric oxide (NO) and dimethylthiourea (DMTU) are two potential compounds play key roles in enhancing tolerance against a variety of abiotic stresses in plants. In the present study, ameliorating abilities of both NO and DMTU against As‐induced disturbances in Glycine max L. have been investigated. Overall results revealed that As accrual significantly suppressed seed germination, radicle length, and biomass accumulation. The oxidative stress markers such as membrane integrity, contents of reactive oxygen species, and malondialdehyde, and lipoxygenase activity were enhanced by As treatment. Pronounced decreases in protein and DNA contents with a concomitant increase in DNA oxidation were observed in the growing radicles after exposure to As. In addition, treatment of As also revealed elevation in the activity and gene expression of pyrroline‐5‐carboxylate synthetase, in parallel to both proline and sugar accumulation. However, the toxic effects of As were partially/ to a large extent reversed by the exogenous application of NO and DMTU, separately, in Glycine max L. Moreover, between these two treatments, NO was more efficient than the DMTU against As‐induced oxidative stress responses in Glycine max L. Our findings provide a foundation to minimize the toxic effects of As in crop plants worldwide. © 2018 American Institute of Chemical Engineers Environ Prog, 38: 402–409, 2019

show abstract

Plant Responses to Arsenic Toxicity: Morphology and Physiology

Chandrakar

Pandey

Keshavkant

2018

Mechanisms of Arsenic Toxicity and Tolerance in Plants

View full text Add to dashboard Cite

Modulation in arsenic-induced lipid catabolism in Glycine max using proline, 24-epibrassinolide and diphenylene iodonium

Cited by 20 publications

References 35 publications

Modulation of arsenic-induced oxidative stress and protein metabolism by diphenyleneiodonium, 24-epibrassinolide and proline in Glycine max L.

Modulation of arsenic-induced oxidative stress and protein metabolism by diphenyleneiodonium, 24-epibrassinolide and proline in Glycine max L.

Nitric oxide and dimethylthiourea up‐regulates pyrroline‐5‐carboxylate synthetase expression to improve arsenic tolerance in Glycine max L.

Plant Responses to Arsenic Toxicity: Morphology and Physiology

Contact Info

Product

Resources

About