Nitric oxide and dimethylthiourea up‐regulates pyrroline‐5‐carboxylate synthetase expression to improve arsenic tolerance in Glycine max L.

Chandrakar, Vibhuti; Keshavkant, S.

doi:10.1002/ep.12978

Cited by 15 publications

(3 citation statements)

References 34 publications

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“…Changes in root PpP5CS1 gene expression levels. Δ 1 -pyrroline-5-carboxylate synthetase (P5CS) is a key enzyme in the glutamate synthesis pathway of proline (Chandrakar and Keshavkant 2018). Earlier studies have confirmed that host P5CS gene expression could respond to mycorrhizal fungi under abiotic stress conditions (Porcel et al 2004, Abo-Doma et al 2011.…”

Section: Resultsmentioning

confidence: 99%

Mycorrhizal fungi enhance flooding tolerance of peach through inducing proline accumulation and improving root architecture

Zheng¹,

Liang²,

Chu³

et al. 2020

Plant Soil Environ.

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This study aimed to evaluate the effect of an arbuscular mycorrhizal fungus (AMF) Glomus mosseae on plant growth, root architecture, and proline metabolism in roots of peach (Prunes persica L.) under non-flooding and flooding conditions. The 12-day flooding dramatically inhibited root colonisation of G. mosseae, but induced a large number of extraradical mycelia. Although the flooding treatment also relatively inhibited growth and root architecture of peach, the mycorrhizal fungal inoculation dramatically increased shoot and root biomass, plant height, stem diameter, number of 1st- and 2nd-order lateral roots, root total length (mainly 0–1 cm and > 3 cm long), root surface area, and root volume under flooding. The study also revealed distinctly higher proline accumulation in the roots of mycorrhizal plants than non-mycorrhizal plants under both non-flooding and flooding conditions, accompanied by higher Δ1-pyrroline-5-carboxylate synthase (P5CS) activity and lower δ-ornithine transaminase and proline dehydrogenase activities. In addition, the PpP5CS1 gene expression was up-regulated by flooding and mycorrhization. This study concluded that mycorrhizal fungi enhanced flooding tolerance of peach through inducing proline accumulation and improving root architecture.

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Section: Resultsmentioning

confidence: 99%

Mycorrhizal fungi enhance flooding tolerance of peach through inducing proline accumulation and improving root architecture

Zheng¹,

Liang²,

Chu³

et al. 2020

Plant Soil Environ.

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“…Increased proline content in response to SNP treatment is most likely due to up-regulation of P5CS1, which is involved in proline biosynthesis; higher proline levels then prevent As uptake and enhance antioxidant potential [144,145]. As shown by Chandrakar et al [146], both dimethylthiourea and NO provide As tolerance in soybean by up-regulating pyrroline-5-carboxylate synthetase, in parallel with the accumulation of sugars and proline, with NO being more efficient against As-induced oxidative stress (Table 2).…”

Section: Arsenic Stressmentioning

confidence: 92%

Molecular Mechanisms of Nitric Oxide (NO) Signaling and Reactive Oxygen Species (ROS) Homeostasis during Abiotic Stresses in Plants

Wani

Naeem

Castroverde

et al. 2021

IJMS

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Abiotic stressors, such as drought, heavy metals, and high salinity, are causing huge crop losses worldwide. These abiotic stressors are expected to become more extreme, less predictable, and more widespread in the near future. With the rapidly growing human population and changing global climate conditions, it is critical to prevent global crop losses to meet the increasing demand for food and other crop products. The reactive gaseous signaling molecule nitric oxide (NO) is involved in numerous plant developmental processes as well as plant responses to various abiotic stresses through its interactions with various molecules. Together, these interactions lead to the homeostasis of reactive oxygen species (ROS), proline and glutathione biosynthesis, post-translational modifications such as S-nitrosylation, and modulation of gene and protein expression. Exogenous application of various NO donors positively mitigates the negative effects of various abiotic stressors. In view of the multidimensional role of this signaling molecule, research over the past decade has investigated its potential in alleviating the deleterious effects of various abiotic stressors, particularly in ROS homeostasis. In this review, we highlight the recent molecular and physiological advances that provide insights into the functional role of NO in mediating various abiotic stress responses in plants.

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“…The different concentrations of MeJA and IAA were added to the media as described earlier with some modifications ( Zhu et al, 2006 ; Ivanova et al, 2014 ). Dimethylthiourea (DMTU), an H 2 O 2 scavenger, was used at different concentrations according to the earlier methods with some modification ( Xia et al, 2009 ; Chandrakar and Keshavkant, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Alternative Oxidase Inhibition Impairs Tobacco Root Development and Root Hair Formation

Liu

et al. 2021

Front. Plant Sci.

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Alternative oxidase (AOX) is the terminal oxidase of the mitochondrial respiratory electron transport chain in plant cells and is critical for the balance of mitochondrial hemostasis. In this study, the effect of inhibition of AOX with different concentrations of salicylhydroxamic acid (SHAM) on the tobacco root development was investigated. We show here that AOX inhibition significantly impaired the development of the main root and root hair formation of tobacco. The length of the main root of SHAM-treated tobacco was significantly shorter than that of the control, and no root hairs were formed after treatment with a concentration of 1 mM SHAM or more. The transcriptome analysis showed that AOX inhibition by 1 mM SHAM involved in the regulation of gene expression related to root architecture. A total of 5,855 differentially expressed genes (DEGs) were obtained by comparing SHAM-treated roots with control. Of these, the gene expression related to auxin biosynthesis and perception were significantly downregulated by 1 mM SHAM. Similarly, genes related to cell wall loosening, cell cycle, and root meristem growth factor 1 (RGF1) also showed downregulation on SHAM treatment. Moreover, combined with the results of physiological measurements, the transcriptome analysis demonstrated that AOX inhibition resulted in excessive accumulation of reactive oxygen species in roots, which further induced oxidative damage and cell apoptosis. It is worth noting that when indoleacetic acid (20 nM) and dimethylthiourea (10 mM) were added to the medium containing SHAM, the defects of tobacco root development were alleviated, but to a limited extent. Together, these findings indicated that AOX-mediated respiratory pathway plays a crucial role in the tobacco root development, including root hair formation.

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Nitric oxide and dimethylthiourea up‐regulates pyrroline‐5‐carboxylate synthetase expression to improve arsenic tolerance in Glycine max L.

Cited by 15 publications

References 34 publications

Mycorrhizal fungi enhance flooding tolerance of peach through inducing proline accumulation and improving root architecture

Mycorrhizal fungi enhance flooding tolerance of peach through inducing proline accumulation and improving root architecture

Molecular Mechanisms of Nitric Oxide (NO) Signaling and Reactive Oxygen Species (ROS) Homeostasis during Abiotic Stresses in Plants

Alternative Oxidase Inhibition Impairs Tobacco Root Development and Root Hair Formation

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