In this work, we have examined and proposed a dielectrically modulated biosensor based on the dual trench transparent gate engineered MOSFET (DM DT GE-MOSFET) for label-free detection of biomolecules with enhanced sensitivity and efficiency. Different sensing parameters such as the ION/IOFF, threshold voltage shift have been evaluated to validate the sensing metric for the proposed device. Additionally, the SVth (Vth Sensitivity) has been also analyzed by considering the charged (positive and negative) biomolecules. In addition to this, the RF sensing parameters such as the transconductance gain and cut-off frequency have been also taken into account to provide a better insight into the sensitivity analysis of the proposed device. Furthermore, the linearity, distortion and noise immunity of the device has been evaluated to check the overall performance of the biosensor at high frequency (GHz). Moreover, the results indicate that, the proposed biosensor exhibits a SVth of 0.68 for the positively charged biomolecules at a very low drain bias (0.2 V). Therefore, the proposed device can be used as an alternative to the conventional FET-based biosensors.