miR-216b Post-Transcriptionally Downregulates Oncogene KRAS and Inhibits Cell Proliferation and Invasion in Clear Cell Renal Cell Carcinoma

Wang, Yan; Dong, Daoquan; Jiang, Shuhan; Zhang, Enpu; Zheng, Wenzhong; Mao, Longyi; Li, Weiqing; Zhou, Jingcheng; Fan, Longlong; Cheng, Ran; Li, Zesong; Fang, Zhengyu; Gui, Yaoting; Li, Xianxin

doi:10.1159/000493621

Cited by 23 publications

(19 citation statements)

References 26 publications

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“…miRNAs can act as oncomiRs or tumor suppressors in ccRCC [9][10][11]. However, the effects and molecular mechanisms of miR-671-5p in ccRCC progression have yet to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

HMGA1-mediated miR-671-5p targets APC to promote metastasis of clear cell renal cell carcinoma through Wnt signaling

Chi¹,

Meng²,

Ding³

et al. 2020

neo

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This study aimed to investigate the effect of miR-671-5p on metastasis of clear cell renal cell carcinoma (ccRCC) and underlying mechanism involved. The migration and invasion of ccRCC cells were determined by transwell and boyden assays in vitro and in vivo. Genes mRNA and protein expression were detected by quantitative polymerase chain reaction (qPCR) and western blot analysis, respectively. The target gene of miRNA was confirmed by luciferase reporter assays. Transcriptional regulation of miRNA by transcription factor was detected by chromatin immunoprecipitation assay (ChIP). The expressions of miRNA in clinical specimens were detected by in situ hybridization (ISH). miR-671-5p promoted migration and invasion of ccRCC in vivo and in vitro. Moreover, miR-671-5p directly targeted APC to activate Wnt signaling, thus inducing the epithelial-mesenchymal transition (EMT) in ccRCC. Intriguingly, miR-671-5p expression was transcriptionally enhanced by HMGA1. Consistently, bioinformatics analysis suggested that HMGA1 was positively correlated with miR-671 expression; however, miR-671 was negatively correlated with APC. In situ hybridization analysis showed that miR-671-5p was upregulated in ccRCC compared with paracarcinoma and correlated with poor prognosis of ccRCC patients. In addition, univariate and multivariate analysis indicated that miR-671-5p expression was an independent prognostic factor for overall survival in ccRCC patients. Our data suggest that miR-671-5p is a tumor enhancer in regulating of ccRCC metastasis, and miR-671-5p may be utilized as a factor for the clinical diagnosis and prognosis of ccRCC.

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“…miRNAs can act as oncomiRs or tumor suppressors in ccRCC [9][10][11]. However, the effects and molecular mechanisms of miR-671-5p in ccRCC progression have yet to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

HMGA1-mediated miR-671-5p targets APC to promote metastasis of clear cell renal cell carcinoma through Wnt signaling

Chi¹,

Meng²,

Ding³

et al. 2020

neo

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“…Several studies also showed antitumor effects of amygdalin in renal cell carcinoma (RCC) which ranked the second in genitourinary tumors in China . Juengel et al exposed RCC cells A498, Caki‐1and KTC‐26 to amygdalin at a dose of 10 mg/mL for 24 hours or 48 hours and used untreated cells as control.…”

Section: Recent Updates In Research Of the Anti‐tumor Effect Of Amygdmentioning

confidence: 99%

Recent updates and future perspectives about amygdalin as a potential anticancer agent: A review

Shi

Chen

et al. 2019

Cancer Medicine

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The overall incidence of cancer is increasing in recent years. Despite advances in various comprehensive treatments, the mortality of advanced malignant tumors remains at a high level. Numerous pharmacological studies have confirmed that many Chinese herbal medicines possess remarkable antitumor activities. Amygdalin, mainly existing in bitter almond, is reported to have antitumor properties in addition to the antioxidative, antibacterial, anti‐inflammatory and immunoregulatory activities. This article summarizes the structural characteristics of amygdalin, its antitumor mechanisms, and recent progress and achievement in the research of amygdalin, hoping that it could provide theoretical clues for exploring the clinical value of amygdalin against tumors. Amygdalin is known to have an antitumor effect in solid tumors such as lung cancer, bladder cancer and renal cell carcinoma by affecting cell cycle, inducing apoptosis and cytotoxicity, and regulating immune function. Further research is needed to elucidate the pharmacological mechanisms of amygdalin in terms of the optimal dosage, the feasibility of combined use of amygdalin with other antitumor drugs, and even artificial synthesis of the active components in amygdalin, for the sake of enhancing its antitumor activities and reducing its adverse effects for clinical use.

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“…A previous study demonstrated the close relationship between miR-216b and different types of human cancer such as breast cancer, cervical cancer and colorectal cancer (35). miR-216b inhibits proliferation and promotes apoptosis in pancreatic cancer and clear cell renal cell carcinoma by downregulating KRAS (36,37). In colorectal cancer, miR-216b suppresses tumors by activating the JAK2/STAT3 signaling pathway (38).…”

Section: Discussionmentioning

confidence: 98%

Thiostrepton and miR‑216b synergistically promote osteosarcoma cell cytotoxicity and apoptosis by targeting FoxM1

Cai

Xiao²,

Shen³

et al. 2020

Oncol Lett

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Osteosarcoma is a common primary bone cancer that there are currently no effective treatment strategies for. Forkhead box M1 (FoxM1) is key in the development of osteosarcoma, and microRNA (miR)-216b serves an antitumor role by targeting FoxM1. Moreover, thiostrepton (TST), a natural thiazole antibiotic, induces antitumor effects and specifically targets FoxM1. Therefore, the present study investigated whether thiostrepton and miR-216b synergistically inhibited osteosarcoma cells by targeting FoxM1. The MTT assay, reverse transcription-quantitative PCR, a dual-luciferase reporter assay and flow cytometry were performed. Compared with the human osteoblast cell line hFOB1.19, miR-216b expression was significantly downregulated in the osteosarcoma cell lines U2OS, MG63 and Saos-2. By contrast, FoxM1 expression was significantly upregulated in osteosarcoma cell lines compared with the hFOB1.19 cell line. The results indicated that miR-216b targeted the 3'-untranslated region of FoxM1. Moreover, the results suggested that miR-216b cooperated with TST to decrease cell cytotoxicity and increase cell apoptosis. In addition, miR-216b cooperated with TST to increase Bax expression and decrease Bcl-2 expression. In conclusion, the combination of TST and miR-216b synergistically promoted osteosarcoma cell cytotoxicity and apoptosis by targeting FoxM1. Therefore, the present study suggested that the combination of TST and miR-216b may serve as a promising therapeutic strategy for osteosarcoma.

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miR-216b Post-Transcriptionally Downregulates Oncogene KRAS and Inhibits Cell Proliferation and Invasion in Clear Cell Renal Cell Carcinoma

Cited by 23 publications

References 26 publications

HMGA1-mediated miR-671-5p targets APC to promote metastasis of clear cell renal cell carcinoma through Wnt signaling

HMGA1-mediated miR-671-5p targets APC to promote metastasis of clear cell renal cell carcinoma through Wnt signaling

Recent updates and future perspectives about amygdalin as a potential anticancer agent: A review

Thiostrepton and miR‑216b synergistically promote osteosarcoma cell cytotoxicity and apoptosis by targeting FoxM1

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