HMGA1-mediated miR-671-5p targets APC to promote metastasis of clear cell renal cell carcinoma through Wnt signaling

Chi, X G; Meng, Xiangxin; Ding, Dongyan; Xuan, Xiuchen; Chen, Y Z; Cai, Qing; Wang, A

doi:10.4149/neo_2019_190217n135

Cited by 18 publications

(18 citation statements)

References 20 publications

(21 reference statements)

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“…For example, Tan et al ( 9 , 10 ) have demonstrated that miR-671-5p suppresses forkhead box M1-mediated epithelial-to-mesenchymal transition (EMT) during the oncogenesis of breast cancer. Similarly, miR-671-5p exerts suppressive effects on the cell proliferation in esophageal squamous cell carcinoma ( 11 ), osteosarcoma ( 12 ) and clear cell renal cell carcinoma ( 13 ). By contrast, miR-671-5p targets cerebellar degeneration-related autoantigen 1 to promote the proliferation, migration and invasion of glioblastoma cells ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑671‑5p inhibits cell proliferation, migration and invasion in non‑small cell lung cancer by targeting MFAP3L

Luo

et al. 2021

Mol Med Rep

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MicroRNA (miR)-671-5p serves as a tumor suppressor in several types of cancer, including gastric and breast cancer. However, the function of miR-671-5p in non-small cell lung cancer (NSCLC) has not been described in detail. The present study aimed to investigate the role of miR-671-5p in NSCLC. The expression levels of miR-671-5p were determined in NSCLC tissue samples and cell lines using reverse transcription-quantitative PCR. Prediction of miR-671-5p targets was performed using the TargetScan database and verified by luciferase reporter assay and western blot analysis. Functional experiments, including Cell Counting Kit-8, wound healing and Transwell assays, were performed in NSCLC cells. The results of the present study demonstrated that lower expression levels of miR-671-5p were observed in NSCLC tissues and cell lines compared with those in the corresponding controls. Low miR-671-5p levels were significantly associated with an advanced Tumor-Node-Metastasis stage and lymph node metastasis in patients with NSCLC. Microfibril-associated protein 3-like (MFAP3L) was confirmed to be a direct target of miR-671-5p. The proliferative, migratory and invasive abilities of NSCLC cells were suppressed following transfection with miR-671-5p mimics and promoted by the miR-671-5p inhibitor compared with those in the respective control groups. In addition, the effects of miR-671-5p on cell proliferation, migration and invasion, as well as the expression levels of proliferating cell nuclear antigen, E-cadherin, N-cadherin and vimentin were reversed by MFAP3L overexpression. In conclusion, targeting the miR-671-5p/MFAP3L signaling pathway may be a promising therapeutic strategy for NSCLC treatment.

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Section: Introductionmentioning

confidence: 99%

MicroRNA‑671‑5p inhibits cell proliferation, migration and invasion in non‑small cell lung cancer by targeting MFAP3L

Luo

et al. 2021

Mol Med Rep

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“…In these tumors, miR-671 was demonstrated to show inhibit the malignant phenotypes of tumor cells by targeting SOX6, CCND2, and FOXM1 (23)(24)(25)(26)(27). However, miR-671 may function as an oncogenic miRNA in other types of cancer (28,29). In our study, miR-671 was identified as a downstream effector of circ_0092314 in PAAD cells, in which the tumor-promoting effects of c i r c _ 0 0 9 2 3 1 4 c o u l d b e bl o c k e d t h r o u g h mi R -6 7 1 overexpression.…”

Section: Discussionmentioning

confidence: 99%

CircRNA circ_0092314 Induces Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells via Elevating the Expression of S100P by Sponging miR-671

et al. 2021

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BackgroundCircular RNAs (circRNAs) is a novel class of non-coding RNAs that regulate gene expression during cancer progression. Circ_0092314 is a newly discovered circRNA that was upregulated in pancreatic cancer (PAAD) tissues. However, the detailed functions and underlying mechanisms of circ_0092314 in PAAD cells remain unclear.MethodsWe first determined the expression of circ_0092314 in PAAD and normal tissues and further investigated the functional roles of circ_0092314 in regulating epithelial-mesenchymal transition (EMT) of PAAD cells. We also assessed the regulatory action of circ_0092314 on the microRNA-671 (miR-671) and its target S100P.ResultsCirc_0092314 was markedly upregulated in PAAD tissues and cells, and its overexpression was closely correlated with worse prognosis of PAAD patients. Functionally, circ_0092314 promotes proliferation, invasion and EMT in vitro and tumor growth in vivo. Mechanistically, we demonstrated that circ_0092314 directly binds to miR-671 and relieve its suppression of the downstream target S100P, which induces EMT and activates the AKT signaling pathway. The tumor-promoting effects caused by overexpression of circ_0092314 could be revered by re-expression of miR-671 in PAAD cells.ConclusionsOverall, our study demonstrates that circ_0092314 exerts critical roles in promoting the EMT features of PAAD cells, and provides insight into how elevated expression of circ_0092314 might influence PAAD progression.

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“…HMGA1 was a well‐studied transcription factor that involves in cell metastasis and EMT in RCC. Recently, Chi et al (2020) have reported the oncogene HMGA1 transcriptionally enhanced miR‐671‐5p expression to form HMGA1/miR‐671‐5p/APC signaling, thus inducing the EMT process in RCC. Similarly, Dong et al (2020) have also reported the EMT‐regulatory role of HMGA1 in RCC.…”

Section: Discussionmentioning

confidence: 99%

Pseudogene HSPB1P1 contributes to renal cell carcinoma proliferation and metastasis by targeting miR‐296‐5p to regulate HMGA1 expression

Chen

Wang

Chen

et al. 2021

Cell Biology International

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With the aid of next‐generation sequencing technology, pseudogenes have been widely recognized as functional regulators in the development and progression of certain diseases, especially cancer. Our present study aimed to investigate the functions and molecular mechanisms of HSPB1‐associated protein 1 pseudogene 1 (HSPB1P1) in renal cell carcinoma (RCC). HSPB1P1 expression at the mRNA levels was determined by quantitative real‐time polymerase chain reaction, and its clinical significance was assessed. Cell viability was detected by Cell Counting Kit‐8 assay. Cell migration and invasion were detected by transwell assays. The location of HSPB1P1 in RCC cells was detected by subcellular distribution analysis. The direct relationship between HSPB1P1 and miR‐296‐5p/HMGA1 axis was verified by dual‐luciferase reporter assay and RNA immunoprecipitation assay. Our results identify the elevated expression of HSPB1P1 in RCC tissues and cell lines, which predicted advanced progression and poor prognosis in patients with RCC. Knockdown of HSPB1P1 suppressed cell proliferation, migration, and invasion, and reversed epithelial–mesenchymal transition process in RCC. HSPB1P1 was mostly enriched in the cytoplasm and functioned as a miRNA sponge for miR‐296‐5p and then regulated high‐mobility group A1 expression. In conclusion, our study indicated that HSPB1P1 contributed to RCC progression by targeting the miR‐296‐5p/HMGA1 axis, and should be considered as a promising biomarker and therapeutic target for clinical applications.

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HMGA1-mediated miR-671-5p targets APC to promote metastasis of clear cell renal cell carcinoma through Wnt signaling

Cited by 18 publications

References 20 publications

MicroRNA‑671‑5p inhibits cell proliferation, migration and invasion in non‑small cell lung cancer by targeting MFAP3L

MicroRNA‑671‑5p inhibits cell proliferation, migration and invasion in non‑small cell lung cancer by targeting MFAP3L

CircRNA circ_0092314 Induces Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells via Elevating the Expression of S100P by Sponging miR-671

Pseudogene HSPB1P1 contributes to renal cell carcinoma proliferation and metastasis by targeting miR‐296‐5p to regulate HMGA1 expression

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