Mild induced testicular and epididymal hyperthermia alters sperm chromatin integrity in men

Ahmad, Gulfam; Moinard, Nathalie; Esquerré-Lamare, Camille; Mieusset, R.; Bujan, Louis

doi:10.1016/j.fertnstert.2011.12.025

Cited by 58 publications

(62 citation statements)

References 66 publications

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“…In support of our results, it has been shown that sperm DF increased in idiopathic infertile men (Saleh et al, 2003;Appasamy et al, 2007;Mahfouz et al, 2010), varicocele patients (Smith et al, 2006;Wang et al, 2012), infertile men with genitourinary infection (Gallegos et al, 2008), testicular hyperthermia (Ahmad et al, 2012), cigarette smoking (Selit et al, 2013), testicular cancer (O'Flaherty et al, 2008), vasectomy reversal (Smit et al, 2010) and cryptorchidism (Smith et al, 2007). Zhang et al (2009Zhang et al ( , 2010 measured the DF percentage in semen and showed that the amount of DF was higher in infertile groups compared with fertile groups.…”

Section: Discussionsupporting

confidence: 88%

Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

et al. 2012

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The objective of this study was to investigate the association between the amount of superoxide anion, peroxynitrite as oxidative stress (OS) markers and total antioxidant capacity (TAC) with sperm DNA fragmentation in infertile men with abnormal semen parameters. Semen samples were obtained from 102 infertile couples and divided into groups with normal and abnormal semen parameters according to the World Health Organization (WHO). Peroxynitrite and superoxide anions were detected using spectrofluorometric assays combined with 2,7 dicholorofluorescein (DCF)-DA and 4-chloro-7-nitrobenzo-2-oxa -1, 3-diazole (NBD-CL). Colorimetric assay was used for evaluation of TAC, while DNA fragmentation was studied by using sperm chromatin dispersion test. Superoxide anion, peroxynitrite and DNA fragmentation were significantly higher in infertile couples with abnormal semen parameters as compared to infertile couples with normal semen (P < 0.01). TAC was significantly lower in infertile men with abnormal semen parameters (P < 0.01). There was also a significant positive correlation between OS markers with sperm DNA fragmentation (r = 0.59, P < 0.01 and r = 0.67, P < 0.01, respectively). We have found that imbalance between superoxide anion and peroxynitrite with antioxidant capacity in infertile men with abnormal sperm parameters is associated with higher sperm DNA fragmentation.

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Section: Discussionsupporting

confidence: 88%

Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

et al. 2012

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“…hyperthermia may result from wearing close-fitting underwear, sauna use, longtime bathing, and cycling [42][43][44][45]. The position of the scrotum acts to maintain the temperature of the testes (34-35°C) lower than that of the body (36-37°C) [46].…”

Section: External Causes Of Potential Oxidative Stressmentioning

confidence: 99%

“…To minimize exogenous ROS generation, cessation of smoking [89], weight loss through diet education and moderate exercise [90], and decreasing the opportunity of exposure to phthalate [91] are useful preventive measures. It is also well established that alcohol abuse [92], an elevated temperature around the scrotum [44,45], and exposure to toxins such as heavy metals and organic solvents [93] lead to an increase in oxidative stress and can have harmful effects on one's fertile capacity. Avoiding cycling with tight pants, avoiding taking long hot water baths and saunas, and avoiding using a laptop on closed legs will also help to minimize oxidative stress [42][43][44][45].…”

Section: Lifestyle Modificationmentioning

confidence: 99%

“…It is also well established that alcohol abuse [92], an elevated temperature around the scrotum [44,45], and exposure to toxins such as heavy metals and organic solvents [93] lead to an increase in oxidative stress and can have harmful effects on one's fertile capacity. Avoiding cycling with tight pants, avoiding taking long hot water baths and saunas, and avoiding using a laptop on closed legs will also help to minimize oxidative stress [42][43][44][45]. Further, using protective equipment at work places that reduces the exposure to chemicals and vapors leading to oxidative stress is also an effective preventive way to minimize oxidative stress.…”

Section: Lifestyle Modificationmentioning

confidence: 99%

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Reactive Oxygen Species and Sperm Cells

Takeshima¹,

Kuroda²,

Yumura³

2018

Reactive Oxygen Species (ROS) in Living Cells

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Many cases of male factor infertility are idiopathic, but 30-40% of cases may have excessive levels of reactive oxygen species (ROS) in their semen. The origins of endogenous ROS are leukocytes and immature spermatozoa, and external causes are various. On the contrary, seminal plasma contains various antioxidants. Low levels of ROS are essential for the fertilization process, but excessive levels of ROS lead to oxidative stress and can have harmful effects such as lipid peroxidation of a membrane, sperm deoxyribonucleic acid fragmentation, and apoptosis on the fertile capacity. In order to evaluate oxidative stress appropriately, ROS is measured by the chemiluminescence method with neat semen and quantification of 8-OH-2′-deoxyguanosine and malondialdehyde in seminal plasma. Antioxidant potential is often measured using total antioxidant capacity (TAC) assay. The oxidation-reduction potential measured by a MiOXSYS analyzer is a novel, easier, quicker, and less expensive technology to measure oxidative stress. In order to minimize oxidative stress and improve clinical outcomes, sperm-sorting methods, lifestyle modifications, shortening the ejaculatory abstinence, and treatments such as oral antioxidants, varicocelectomy, and testicular sperm extraction are taken into account. As a future prospect, proteomics, metabolomics, and genomics are still developing areas that have the potential to discover new findings and highly sensitive biomarkers.

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“…Li et al, (2015) measured the temperature in boar in the rectum and scrotum at heat stress when the outside temperature reached above 28 °C for more than 3 days and found that the percentage of morphologically abnormal sperm had increased, but the function of the additional glands was not impaired. Elevated temperature in the scrotum causes impaired spermiogenesis, which leads to higher incidence of abnormally developed sperm (Ahmad et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Elimination the Impact of Heat Stress by Supplementation of Antioxidants Into Diet of Duroc Boars

Přibilová

Horký

Nevrkla

et al. 2018

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The aim of this experiment was to find out, if is it possible to eliminate the impact of heat stress on quality of Duroc boar ejaculate by the addition of complex of antioxidants (Selenium, Zinc, Vit. C and Vit. E) into the basic diet. The experiment was carried out at the station of insemination in Velke Mezirici (Czech Republic) from May to September. For the study were chosen 12 Duroc boars, of approximately the same age and weight, and were divided into two groups. The control group (n = 6) was fed only by basic diet, the experimental group (n = 6) was fed by basic diet with the supplementation of antioxidants in amount: 0.5 mg selenium (seleno-methionine), 70 mg zinc (zinc-methionine), 70 mg vit. E (alpha-tocopherol) and 350 mg vit. C (L-ascorbic acid) per kilogram of basic diet. Monitored parameters of ejaculate was volume of ejaculate (ml), concentration of sperm (10 6 / ml), total rate of sperm (10 9 ), motility of sperm (%) and amount of morphologically abnormal sperm (%). The control group reported slight increase in volume of ejaculate (of 19 %), which is common in summer months. At the same time was the concentration of sperm unstable, during August there was an increase of 15 %, but during September concentration decreased below the initial amount. Motility was around 69 % during whole experiment. There was an increase (of 15 %) in amount of morphologically abnormal sperm, but the result was insignificant. The experimental group had a higher volume of ejaculate, but the diagram of volume of ejaculate was almost the same with the control group. Concentration of sperm increased after the supplementation of antioxidants (of 23 %) and during the experiment decreased only slightly. Motility was about average amount 70 % and was not various during the experiment. In amount of morphologically abnormal sperm there was an increase about 12 %. According to the results, the supplementation of antioxidants had no significant effect on improving of semen quality. But the addition could have a positive effect to stable the parameters of ejaculate.

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Mild induced testicular and epididymal hyperthermia alters sperm chromatin integrity in men

Cited by 58 publications

References 66 publications

Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

Reactive Oxygen Species and Sperm Cells

Elimination the Impact of Heat Stress by Supplementation of Antioxidants Into Diet of Duroc Boars

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