Relationship of seminal reactive nitrogen and oxygen species and total antioxidant capacity with sperm DNA fragmentation in infertile couples with normal and abnormal sperm parameters

Abstract

The objective of this study was to investigate the association between the amount of superoxide anion, peroxynitrite as oxidative stress (OS) markers and total antioxidant capacity (TAC) with sperm DNA fragmentation in infertile men with abnormal semen parameters. Semen samples were obtained from 102 infertile couples and divided into groups with normal and abnormal semen parameters according to the World Health Organization (WHO). Peroxynitrite and superoxide anions were detected using spectrofluorometric assays combined with 2,7 dicholorofluorescein (DCF)-DA and 4-chloro-7-nitrobenzo-2-oxa -1, 3-diazole (NBD-CL). Colorimetric assay was used for evaluation of TAC, while DNA fragmentation was studied by using sperm chromatin dispersion test. Superoxide anion, peroxynitrite and DNA fragmentation were significantly higher in infertile couples with abnormal semen parameters as compared to infertile couples with normal semen (P < 0.01). TAC was significantly lower in infertile men with abnormal semen parameters (P < 0.01). There was also a significant positive correlation between OS markers with sperm DNA fragmentation (r = 0.59, P < 0.01 and r = 0.67, P < 0.01, respectively). We have found that imbalance between superoxide anion and peroxynitrite with antioxidant capacity in infertile men with abnormal sperm parameters is associated with higher sperm DNA fragmentation.

Peroxynitrite-mediated nitrosative stress decreases motility and mitochondrial membrane potential in human spermatozoa

“…Also, negative associations were found between ONOO 2 levels and Na + /K + -ATPase and Ca 2+ -ATPase activity, and total thiol content (Vignini et al, 2009). A positive correlation has also been reported between RNS and sperm DNA fragmentation (Khosravi et al, 2012). It has been suggested that ONOO 2 generation is the result of excessive activity of nitric oxide synthase and nitric oxide formation, which would cause the decreased sperm function observed in patients with varicocele (Mitropoulos et al, 1996).…”

“…In asthenozoospermic infertile men, higher levels of ONOO 2 and nitration of the tyrosine residues have been found than in normospermic fertile men (Vignini et al, 2006;Salvolini et al, 2012). A negative correlation has been reported between ONOO 2 concentration and sperm morphology and motility (Vignini et al, 2006;Khosravi et al, 2012). Also, negative associations were found between ONOO 2 levels and Na + /K + -ATPase and Ca 2+ -ATPase activity, and total thiol content (Vignini et al, 2009).…”

Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: Possible role in recurrent pregnancy loss

“…Among the various causative agents unravelled, oxidative stress has always been concomitant with nonspecific DNA fragmentation [Aitken et al 2010;de Castro et al 2015;Iommiello et al 2015;Khosravi et al 2014;Muratori et al 2015;Noblanc et al 2013;Wright et al 2014]. This yields an atypical metabolically unbalanced state in which supra-physiological production of reactive oxygen species (ROS) overwhelms the impaired antioxidant defence.…”

Effect of selenium, vitamins E and C on antioxidant potential and quality of boar ejaculate

“…Shapoval et al, 2003). Reactive oxygen forms can damage sperm compatibility and thus reduce their viability (Khosravi et al, 2014;Horký et al, 2012b). So the proper antioxidant system activity in the ejaculate plays an important role in keeping boars reproduction properties.…”

“…The other enzymes from antioxidant system which play an important role in boar ejaculate are: catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST) and phospholipid hydroperoxide glutathione peroxidase (PHGPx) (Koziorowska-Gilun et al, 2011;Horký, 2014). The antioxidants may improve ejaculate quality (Khosravi et al, 2014). The forms of reactive oxygen negatively affect the sperm quality and cause the lipid peroxidation (Ashrafi et al, 2013).…”