Reactive Oxygen Species and Sperm Cells

Takeshima, Teppei; Kuroda, Shinnosuke; Yumura, Yasushi

doi:10.5772/intechopen.73037

Cited by 15 publications

(16 citation statements)

References 115 publications

(117 reference statements)

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“…According to the obtained results, the recombinant FSH therapy can be a routine and experimental clinical treatment, which increases sperm concentration and spermatogonia in people with low semen concentration, but with normal plasma gonadotropin. This treatment is also proven to be capable of improving fertility outcomes of Assisted Reproductive Technique (ART) in oligozoospermia [3,10,11,13,[18][19][20]. In line with the studies mentioned above, in this study, the DFI level of >30% significantly decreased in the oligozoospermia patients treated with rhFSH (75 IU) for three months.…”

Section: Discussionsupporting

confidence: 80%

“…ROS induces lipid peroxidation in the sperm membrane, and peroxidation of the resulting fatty acids has toxic effects on sperms and decreases sperm function [2]. Lipid peroxidation leads to the development of several products, Most Notably Malondialdehyde (MDA) and isoprostane F2α [3][4][5]. The most commonly used biomarker for lipid peroxidation in sperms is MDA [4].…”

Section: Introductionmentioning

confidence: 99%

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The effect of rhFSH on Sperm DNA Fragmentation and sperm parameters in Oligozoospermia Infertile Men

et al. 2020

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Background: One of the main causes of male infertility is the negative effects of oxidative stress. Follicle-stimulating hormone (FSH) plays an essential role in spermatogenesis, as well as in the maintenance of sperm DNA integrity. This study aimed to determine whether the recombinant human follicle-stimulating hormone (rhFSH) treatment of sperm parameters could positively affect sperm DNA and oxidative DNA fragmentation in oligozoospermia infertile men. Materials and Methods: This interventional study was carried out on a sample of 50 oligozoospermia infertile men. To this end, sperm DNA fragmentation and ROS as an oxidative stress marker were measured before and after treatment with the rhFSH sperm parameters. Results: The sperm parameters (concentration, mobility, and morphology) were significantly different in the oligozoospermia infertile patients before and after the rhFSH treatment (p<0.05). Moreover, sperm DNA fragmentation had a significant decrease in the patients after the FSH treatment (p<0.05). In addition, the ROS level in sperm, and the malondialdehyde level of seminal plasma significantly decreased after the treatment (P<0.05). Conclusion: The findings indicated that the rhFSH treatment significantly improved the sperm parameters. Further, the treatment led to a meaningful reduction of sperm DNA fragmentation and oxidative stress in the oligozoospermia infertile patients. Similarly, the malondialdehyde concentration markedly decreased in correlation with DNA fragmentation after the rhFSH treatment.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

The effect of rhFSH on Sperm DNA Fragmentation and sperm parameters in Oligozoospermia Infertile Men

et al. 2020

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“…In viable and fertile sperm, both in fresh and diluted semen, ROS levels, and membrane lipid peroxidation are low [25]. At low levels, ROS plays a decisive role in tyrosine phosphorylation, sterol oxidation, and cholesterol efflux from the plasma membrane in the spermatozoon capacitation and the fertilization process [26].…”

Section: Viability Motility and Dose For Intracervical Inseminationmentioning

confidence: 99%

“…Susceptibility to cold temperatures is associated with a higher ratio of unsaturated fatty acids than saturated fats, which results in the formation of a highly ROS [30]. One of the products of lipid peroxidation is MDA [26], which increases in long-term storage.…”

Section: Ipm and Malondialdehyde Levelmentioning

confidence: 99%

Addition of L-arginine in skim milk extender maintains goat spermatozoa quality in chilled temperature for five days

et al. 2019

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Aim: The purpose of this study was to determine the benefits of L-arginine addition in skim milk extender to maintain the quality of goat spermatozoa in chilled storage. Materials and Methods: A total of 18 ejaculates from three healthy goats with weight and age of 45 kg and 4-5 years, respectively, were divided into three groups. The control group contained goat semen diluted in a skim milk extender without L-arginine; Treatment I and Treatment II contained goat semen diluted in a skim milk extender with added L-arginine 4 and 6 mM, respectively. These three groups were chilled at 5°C and evaluated daily for 5 days. Observed variables were viability, motility, intact plasma membrane (IPM), malondialdehyde (MDA) level, necrosis, and apoptosis of spermatozoa. Results: The addition of L-arginine 4 mM was the best treatment in maintaining viability, motility, and IPM and a decreased MDA level, percentage of necrosis, and apoptosis of goat spermatozoa. An ejaculate in this extender can be divided into 37 doses for intracervical insemination in <1 ml volume with 125 million motile spermatozoa. Conclusion: Goat semen retained its quality when kept for 5 days in chilled storage by adding L-arginine in skim milk extender.

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“…Free radicals are also required for normal cell proliferation, differentiation, and intracellular signaling that occurs in the process of migration. Excessive free radical formation often results in a spermiogenesis error, which causes abnormally high levels of cytoplasmic retention to be released from the germinal epithelium (Sanocka and Kurpisz, 2004).…”

Section: Free Radicalsmentioning

confidence: 99%

Evaluation of Current Antioxidant Profile in Semen

2021

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The freezing and storage of the sperm are used cryopreservation of germplasm in livestock breeding, genetic improvement of indigenous species, preservation of rare races, successful tolerance to environmental changes and international germplasm exchanges. Both the freezing and thawing process causes large changes in the volume of the cell fluid. Spermatozoon removes most of its cytoplasm at differentiation stages and lacks the cytoplasmic component that contains antioxidants that counteract the harmful effect of reactive oxygen species and lipid peroxidation. Therefore, the sensitivity of spermatozoa to lipid peroxidation increases during the freezing and thawing of the sperm, which creates a significant mechanical stress on the cell membrane. Oxidative stress is caused by oxygen and oxygen-derived oxidants, commonly known as ROS, and is known as an imbalance between the ability of biological systems to easily detoxify or repair damaged reagents. Uncontrolled ROS production, which exceeds the antioxidant capacity of seminal plasma, causes oxidative stress that is harmful to spermatozoa. All cellular components, including lipids, proteins, nucleic acids, and sugars, are potential targets of oxidative stress. Antioxidants control the chemical degradation of the substrate caused by oxidation, neutralizing free radicals, thereby it is used to minimize the risk of damage to spermatozoa during cryopreservation.

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Reactive Oxygen Species and Sperm Cells

Cited by 15 publications

References 115 publications

The effect of rhFSH on Sperm DNA Fragmentation and sperm parameters in Oligozoospermia Infertile Men

The effect of rhFSH on Sperm DNA Fragmentation and sperm parameters in Oligozoospermia Infertile Men

Addition of L-arginine in skim milk extender maintains goat spermatozoa quality in chilled temperature for five days

Evaluation of Current Antioxidant Profile in Semen

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