Micropropagation of Oregano (Origanum syriacum L.) Through Tissue Culture Technique

Abstract

This research was carried out at the Plant Tissue Culture Laboratory (PTCL) in Faculty of Environmental Agricultural Sciences, Arish University, Egypt during 2013 to 2016. The aim of this study was using tissue culture technique for the micropropagation of Origanum syriacum L. plant. One node cutting and shoot tip were cultured on Murashige and Skoog (MS), Nitsch and Nitsch (NN) and Gamborg (B5) media. The results showed that shoot tip cultured on the MS medium had the best combination for the establishment stage of mother plants. Shoots cultured on MS medium supplemented with0.50 mgl -1 Kin and 0.05 mgl -1 NAA produced the highest shoots developed during multiplication stage. MS medium supplemented with 0.40 mgl -1 thiamine achieved the best shoots development compared with the other additive treatments and control. Addition of 1 mgl -1 IBA to the full strength MS medium and 1.00 mgl -1 IAA to the half strength MS had the highest number of main roots and root lengths. Hardening the produced rooted plantlets were done in a greenhouse in pots containing mixture of peatmoss, vermiculite and washed sand at the rate (1:1:1) and after adjustment of mixture pH. Plantlets were successfully acclimated with 93 % survive.

Effect of Macronutrients, Cytokinins and Auxins, on <i>in Vitro</i> Organogenesis of <i>Thymus vulgaris</i> L.

“…Furmanowa and Olszowska [15], the first to establish micropropagation of Thymus vulgaris, used Nitsch and Nitsch (1969) [44] medium. Moreover, our results are in concordance with Abdallah et al [45], who obtained the best number of shoots, leaves and leaves/shoots for Origanum syriacum on MS medium, compared to NN and B 5 , as well as with Ӧzkum [46], who found that MS generates the highest number of parameters, such as the number of shoots and the number of leaves/shoots for Origanum minutiflorum, compared to B 5 . Also, Nordine et al [47] found that MS medium is the best for survival, multiplication and elongation of Thymus hyemalis Lange shoots, compared to 1/2 MS, B 5 and White (1963) [48].…”

Application of Tissue Culture Technique on Marrubium vulgare L. Plant

“…Abdallah (2012) found that 0.30 mgl -1 of pyridoxine resulted in the highest number of shoots, shoot length, number of leaves and number of leaves/shoot of Capparis spinosa. Also, Abdallah (2017) found that 0.40 mgl -1 thiamine achieved the highest values of number of shoots, shoot length, number of leaves and number of leaves/shoot on Origanum syriacum. Arafeh et al (2003), Oluk and Cakir (2009) observed that plants were rooted on 1/2 strength MS medium supplemented with0.10 -2.00 mgl -1 IBA for Origanum species.…”

Efficient Micropropagation protocol for Lamium garganicum L. subsp. striatum (Sm.) Hayek var. striatum Grown Naturally in Turkey

“…Acclimatized and survival plantlets There has been many reports published about the Turkish medicinal and aromatic species represented in Lamiaceae family such as Origanum sp. (Avtar and Çölgeçen 2019;Abdallah et al 2017;Korkor et al 2017;Sevindik et al 2017;El Beyrouthy et al 2015;Hussein et al 2014;Yıldırım et al 2013;Yashodabai et al 2011;Özkum et al 2007;Arafeh et al 2006;El-Gengaihi et al 2006;Goleniowski et al 2003;Iyer and Pai 2000;Iconomou-Petrovich and Nianiou-Obeidat 1998;Iyer and Pai 1998;Sajina et al 1996), Sideritis sp. (Sevindik et al 2019;Yavuz 2016;Shtereva et al 2015;Faria et al 1998;Sánchez-Gras and Segura 1997;Garcia-Granados et al1994;Sánchez-Gras and Segura 1988), but only a few report were published about in vitro propagation of Lamium sp.…”