Micropropagation and validation of genetic homogeneity of Alhagi maurorum using SCoT, ISSR and RAPD markers

Agarwal, Tanvi; Gupta, Amit; Patel, Ashok Kumar; Shekhawat, N. S.

doi:10.1007/s11240-014-0608-z

Cited by 78 publications

(29 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For ISSR (UBC series, University of British Columbia, Vancouver, Canada) amplification, PCR was performed in a 15 ll reaction mixture having the same composition as described for RAPD primers. For PCR amplification, thermal cycler was programmed as described by Agarwal et al (2015).…”

Section: Issr Analysismentioning

confidence: 99%

An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

Choudhary

Patel

Harish

et al. 2017

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA). The maximum number of shoots (45.30 ± 3.83) with an average length 6.52 ± 0.89 cm were differentiated on MS medium containing 0.5 mg L BAP, 0.1 mg L IAA and additives (50 mg L ascorbic acid, 25 mg L each of adenine sulphate, citric acid and l-arginine). The cloned shoots were rooted ex vitro. Each shoot treated with 250 mg L IBA for 5 min produced 12.3 ± 1.33 with a mean length 5.4 ± 0.73 cm. More than 85% (46 plants) of ex vitro rooted plantlets were successfully hardened in a greenhouse with normal growth characteristics. In order to evaluate the genetic stability of micropropagated plants, the two PCR-based techniques, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used. The amplification patterns of the micropropagated and mother plant were monomorphic thus depicting genetic stability of the micropropagation system. This protocol could be effectively employed for the mass multiplication of wild female , a popular summer vegetable crop.

show abstract

Section: Issr Analysismentioning

confidence: 99%

An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

Choudhary

Patel

Harish

et al. 2017

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…Figure 1 is a schematic representation of the pitaya in vitro propagation procedure, including the approximate time required for each step. SCoT, a simple and novel DNA marker, has been used for genetic diversity analysis of micropropagated plants due to its high reproducibility and sensitivity (Agarwal et al 2014;Rathore et al 2014). Results from SCoT analyses showed that there was no polymorphic bands among propagated plants and parental plants ( Fig.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

A protocol for rapid in vitro propagation of genetically diverse pitaya

Hua

Chen

et al. 2014

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

An efficient propagation system of pitaya with diverse genetic background was established by using tender stem as explants. Various types of plant growth regulators (PGRs) were used to determine the most effective hormone combination for shoot proliferation. Zeatin (ZT), thidiazuron (TDZ), 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (6-BA) alone could induce multiple shoots. In that case, the most shoots per explant i.e. 4.6 (13.68 lM ZT), 4.8 (0.11 lM TDZ), 4.3 (17.76 lM 6-BA) and 3.2 (0.23 lM 2, 4-D) were obtained. The best PGRs combination was MS medium with 3.0 lM ZT and 0.5 lM IBA producing the most shoots per explant and the most vigorous shoots. Of the two varieties and six superior selections cultured on the best PGRs combination, more than 6.0 shoots per explant were obtained. No polymorphism was detected among in vitro-derived plantlets selected at random after 11 sub-cultures. Those results showed that MS media with 13.68 lM ZT and 2.46 lM IBA was suitable for shoot propagation of diverse pitaya varieties and selections. The protocol can be used for largescale propagation of pitaya to meet the demand of increasing commercial cultivation.

show abstract

“…In general, the in vitro plant regeneration procedure involved the use of growth regulators even when axillary buds were used as explants [19,20,48,49,50]. In L. alba, for example, the cytokinin BAP has been used in the cultivation of nodal segments with the purpose of rapid multiplication [39,40,41].…”

Section: Discussionmentioning

confidence: 99%

“…Among the most used markers, ISSR and SSR have been largely used due to its simplicity, speed, and cost-effectiveness being highly discriminative and reliable [15,16,17,18]. These markers have been very useful to investigate the genetic stability of several micropropagated species such as Gossypium hirsutum [15], Platanus acerifolia [19], Alhagi maurorum [20], Gerbera jamesonii [16], Pisum sativum [21], Zea mays [17], Bacopa monnieri [14], and Achras sapota [22].…”

Section: Flow Cytometry and Molecular Markers Havementioning

confidence: 99%

Genome Status of Lippia alba Polyploid Complex Long-term in vitro Cultivated

Julião

Lopes

Zorzatto

et al. 2020

EJMP

View full text Add to dashboard Cite

This study is the first report of a genetic stability analysis of a polyploid complex maintained in vitro for a long-time. Twenty-two accessions of Lippia alba, a medicinal species of economic importance, had been maintained under in vitro culture conditions for 7 years through sprouting of axillary buds. Four clones of each accession were analyzed, being three plants from in vitro bank and one cultivated in the field. We investigated the genetic stability of diploid, aneuploid, triploid, tetraploid, and hexaploid accessions. The investigation was carried out using flow cytometry, inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers. No significant variation in nuclear DNA content was observed between the in vitro conserved plants and their respective field plant. Out of 23 ISSR primers screened, 8 primers were found to produce clear reproducible bands resulting in a total of 5456 bands. 86.36% of the analyzed plantlets (19 accessions) showed at least one polymorphic band. The polymorphic rate ranged from 1.61 to 33.87%. The SSR markers were used to confirm the absence or low occurrence of variation in accessions that showed no polymorphism or polymorphism for only one ISSR primer. The genetic instability detected in our study at the molecular level may be attributed to the natural instability of L. alba genome combined with the long-time in vitro maintenance.

show abstract

Micropropagation and validation of genetic homogeneity of Alhagi maurorum using SCoT, ISSR and RAPD markers

Cited by 78 publications

References 36 publications

An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

A protocol for rapid in vitro propagation of genetically diverse pitaya

Genome Status of Lippia alba Polyploid Complex Long-term in vitro Cultivated

Contact Info

Product

Resources

About