An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

Abstract

Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA). The maximum number of shoots (45.30 ± 3.83) with an average length 6.52 ± 0.89 cm were differentiated on MS medium containing 0.5 mg L BAP, 0.1 mg L IAA and additives (50 mg L ascorbic acid, 25 mg L each of adenine sulphate, citric acid and l-arginine). The cloned shoots were rooted ex vitro. Each shoot treated with 250 mg L IBA for 5 min produced 12.3 ± 1.33 with a mean length 5.4 ± 0.73 cm. More than 85% (46 plants) of ex vitro rooted plantlets were successfully hardened in a greenhouse with normal growth characteristics. In order to evaluate the genetic stability of micropropagated plants, the two PCR-based techniques, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used. The amplification patterns of the micropropagated and mother plant were monomorphic thus depicting genetic stability of the micropropagation system. This protocol could be effectively employed for the mass multiplication of wild female , a popular summer vegetable crop.

Genetic characterization of Gac (Momordica cochinchinesis) accessions in Southern Vietnam by ISSR markers

“…The tested primers generated from 3 to 12 amplifications per reaction and the length of amplification varies approximately from 150 to 2,000 bp. Our results are consistent with study of Pham and colleagues in 2017, where the genetic correlation of 20 gac samples was characterized by using 10 RAPD primers and resulting 126 bands; however the amplified bands in this study is relatively higher than study of Choudhary and colleagues when they used ISSR to evaluate the genetic homogeneity of different Momordica dioica plants with 2-4 bands per primer (Pham et al 2017;Choudhary et al 2017). Among those, there were 114 polymorph bands, and an average of 11.4 bands per primer.…”

Maximization of Micropropagule Production in an Attractive Ornamental Plant, Oxalis triangularis A.st.-Hil

“…The higher concentrations of auxins decreased the number of roots and length considerably. The application of reduced strength of nutrient salts in the MS medium is reported to induce need to develop roots [31]. As per Kiba and Krapp [32] low nitrogen availability with specific plant growth regulators evoke the development of primary and lateral roots.…”

Micropropagation of arrow cane, Gynerium sagittatum (Aubl.) P. Beauv. cv. Criolla, Criolla 1, and Martinera, in a double-phase medium

“…Under in vitro conditions, plant medium and the environment prevent plant tissue from dehydration and favor nutrient uptake without root formation. However, for ex vitro conditions, in vitro rooting provides the mechanisms for normal growth and development of the plant in open field conditions (Choudhary et al, 2017;Zakavi et al, 2016). In vitro adventitious root formation is usually promoted by auxin supply in the culture medium (Ozdemir et al, 2014;Resende et al, 2016).…”