A protocol for rapid in vitro propagation of genetically diverse pitaya

Abstract

An efficient propagation system of pitaya with diverse genetic background was established by using tender stem as explants. Various types of plant growth regulators (PGRs) were used to determine the most effective hormone combination for shoot proliferation. Zeatin (ZT), thidiazuron (TDZ), 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzylaminopurine (6-BA) alone could induce multiple shoots. In that case, the most shoots per explant i.e. 4.6 (13.68 lM ZT), 4.8 (0.11 lM TDZ), 4.3 (17.76 lM 6-BA) and 3.2 (0.23 lM 2, 4-D) were obtained. The best PGRs combination was MS medium with 3.0 lM ZT and 0.5 lM IBA producing the most shoots per explant and the most vigorous shoots. Of the two varieties and six superior selections cultured on the best PGRs combination, more than 6.0 shoots per explant were obtained. No polymorphism was detected among in vitro-derived plantlets selected at random after 11 sub-cultures. Those results showed that MS media with 13.68 lM ZT and 2.46 lM IBA was suitable for shoot propagation of diverse pitaya varieties and selections. The protocol can be used for largescale propagation of pitaya to meet the demand of increasing commercial cultivation.

High-efficiency Micropropagation of Dormant Buds in Spine Base of Red Pitaya (Hylocereus polyrhizus) for Industrial Breeding

“…In this study, addition of 6-BA along with auxin considerably increased morphogenetic response of red pitaya shoots as well as shoot number. Interestingly, the phytohormone combinations for the micropropagation of white pitaya (Hylocereus undatus) (Mohamed-Yasseen, 2002;Hua et al, 2015) were distinctly different from our findings.…”

Pitaya, an Attractive Alternative Crop for Mediterranean Region

“…which have demonstrated good adaptation and produced quality fruits in Mediterranean region. Several protocols were developed for propagation of pitaya [38][39][40][41][42].…”

“…However, tissue culture responses of micropropagated explants are controlled by diverse genetic mechanisms and dragon fruit cultivars with diverse genetic backgrounds respond differently to culture medium conditions [40][41][42]. Thus, an efficient method for micropropagation of the two genetically different pitaya cultivars object of this study was developed to produce multiple adventitious shoots directly from preformed areole buds while minimizing somaclonal variation.…”

Rápida produção de mudas de pitaia (Hylocereus undatus, Cactaceae) por meio da técnica da micropropagação

“…Além do meio de cultura, responsável pela sustentação do explante e pelo fornecimento de nutrientes, a utilização de reguladores de crescimento é essencial para o desenvolvimento dos explantes, pois está intimamente relacionada com a divisão celular e o crescimento (REIS et al, 2008). O uso e o efeito de citocininas na indução de brotações in vitro de pitaias vêm sendo relatados por vários autores (VIÑAS et al, 2012;MENEZES et al, 2012;FAN et al, 2013;HUA et al, 2015). Diferentes resultados são encontrados, de acordo com as concentrações e/ou as espécies trabalhadas.…”