Micropropagação de violeta-africana (Saintpaulia ionantha Wendl.): efeito da Benzilaminopurina na multiplicação

Lucas, M. A. K.; Fagundes, Joelma Dutra; Pereira, Denise Dias; Sarmento, Marcelo Benevenga

doi:10.1590/s1413-70542007000500016

Cited by 5 publications

(7 citation statements)

References 4 publications

(3 reference statements)

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“…A similar result was found for Mentha x Piperita, in which there was a calogenesis above 77% of the explants when using concentrations of 0.5 mg GA 3 L -1 of and 2 mg BAP L -1 (MORAIS et al, 2014). For Saintpaulia ionantha, the presence of callus was observed only at concentrations higher than 0.4 mg BAP L -1 (LUCAS et al, 2007). Thus, the composition of the medium and the concentrations of phytoregulators are essential to obtain high multiplication rates and quality explants.…”

Section: Resultssupporting

confidence: 82%

“…In Egletes viscosa, the highest height of the plants occurred with the use of 0.5 mg GA 3 L -1 of, and absence of BAP in the culture medium, with the addition of cytokinin, causing a reduction in height growth of plants (DINIZ et al, 2003). The increase in the concentration of cytokinin in the medium causes an increase in the number of shoots and a decrease in their height (LUCAS et al, 2007;MORAIS et al, 2014). In contrast to that observed in the present study, in Sinningia speciosa, the height of shoots linearly decreased with increasing BAP concentrations in the culture medium (ARAÚJO et al, 2004), similar to that observed for Saintpaulia ionantha, where the highest shoot height occurred when there was no addition of BAP to the culture medium (LUCAS et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

“…Sinningia speciosa, a species belonging to the same family, the highest bud production (3.5) also occurred when using the BAP concentration of 2 mg L -1 (ARAÚJO et al, 2004). For Saintpaulia ionantha, also belonging to the family Gesneriaceae, the concentration 0.4-1.0 mg BAP L -1 provided the highest multiplication rate for the tested cultivars (LUCAS et al, 2007). Chirita moonii produced 12 buds per initial explant using 3 mg BAP L -1 in a two-month period (HERATH, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Chirita moonii Gardn. species belonging to Gesneriaceae family, there are significant variations in the response of the application of BAP for the multiplication of explants (ARAÚJO et al, 2004;LUCAS et al, 2007;HERATH, 2013), confirming the need for adjustments in the concentration of phytoregulators for each species.…”

Section: Introductionmentioning

confidence: 99%

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Multiplicação in vitro de Codonanthe devosiana

Emer¹,

Winhelmann²,

Grzeça³

et al. 2018

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Codonanthe devosiana is a species endemic to Brazil belonging to the family Gesneriaceae. It features ornamental potential for use in hanging pots and shaded environments. The aim is to evaluate the effects of the combination of different concentrations of BAP and GA3 on the multiplication and development of in vitro explants of C. devosiana. We used MS media supplemented with concentrations of 0.0, 0.2 and 0.4 mg L-1 of BAP and 0.0, 0.25 and 0.50 mg L-1 of GA3 . The number, bud dry matter and height, callus dry matter and presence of roots were evaluated in agglomerates with multiple buds. The interaction between BAP and GA3 concentrations was verified for number of shoots, shoot dry matter and callus dry matter. For shoot height and root formation, only BAP concentrations were observed. The highest number of shoots (4.8) occurred when combined with the concentration of BAP 0.2 mg L-1 and 0.5 mg L-1 GA3 . The lowest shoot height was observed without the use of BAP, as well as the greatest rooting. It is concluded that the combination of 0.2 mg L-1 of BAP with 0.5 mg L-1 of GA3 allows for a more efficient multiplication of C. devosiana explants

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiplicação in vitro de Codonanthe devosiana

Emer¹,

Winhelmann²,

Grzeça³

et al. 2018

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“…Portanto, são importantes para formação de órgãos, principalmente aéreos. A benzilaminopurina (BAP) é uma citocinina sintética utilizada para a multiplicação in vitro de diversas espécies (Lucas et al, 2007).…”

Section: Introductionunclassified

Concentrações de BAP sobre a proliferação in vitro de brotos de Lippia alba [(Mill.)N.E.Brown]

Asmar

Resende

Araruna

et al. 2012

Rev. bras. plantas med.

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Lippia alba é uma planta de origem brasileira, pertencente à família Verbenaceae. É conhecida por combater a insônia e a asma, além de possuir ação anticonvulsivante e antifúngica. A micropropagação tem sido utilizada para multiplicação de várias espécies com propriedades medicinais, e o BAP é a citocinina sintética mais utilizada para a multiplicação in vitro. O objetivo deste trabalho foi estabelecer a concentração adequada de BAP na multiplicação in vitro de L. alba. Explantes constituídos de segmentos nodais provenientes de plântulas já estabelecidas in vitro com aproximadamente 0,5 cm foram inoculados em meio MS, suplementado com 0,0; 0,5; 1,0 e 1,5 mg L-1 de BAP em adição de 30 g L-1 de sacarose. A utilização de 1,5 mg L-1 de BAP promove a multiplicação in vitro de L. alba. A ausência deste regulador propicia maior número de folhas e o uso na concentração de 0,5 mg L-1 aumentou as massas fresca e seca de parte aérea nesta espécie.

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Micropropagação de sucupira-preta por meio de gemas axilares

Moura

Titon

Fernandes

et al. 2012

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar o uso de reguladores de crescimento, aditivos antioxidantes, tipo de explantes e intensidade de cobertura de mudas aclimatizadas, na micropropagação de sucupira-preta (Bowdichia virgilioides) por meio de gemas axilares. Os explantes foram cultivados para a multiplicação em meio de cultura básico WPM, suplementado com concentrações de benzilaminopurina (BAP). No alongamento, os tratamentos foram combinações de ácido naftalenoacético (ANA) e BAP adicionadas ao meio. Para o enraizamento, as brotações foram colocadas em meio com concentrações de ácido indolbutírico, ou em meio com combinações dos aditivos polivinilpirrolidona e carvão ativado, em diferentes concentrações de ANA. As plantas foram transplantadas para copos de plástico, com substrato, e cobertas com saco de polietileno. Posteriormente, esses sacos foram retirados, ou perfurados, ou não retirados, o que constituiu os tratamentos de pré-aclimatização in vitro. A aclimatização ex vitro foi realizada após o período de pré-aclimatização in vitro. A sucupira-preta apresentou melhor micropropagação com emprego de: segmentos cotiledonares e 0,3 mg L -1 de BAP, na multiplicação; 0,3 mg L -1 de ANA e 0,03 mg L -1 de BAP, no alongamento; e carvão ativado (2,0 mg L -1 ), no enraizamento e na pré-aclimatização in vitro, com uso de cobertura de plástico transparente em torno da muda.Termos para indexação: Bowdichia virgilioides, cultura de tecidos, espécie nativa, propagação, reguladores de crescimento. Sucupira-preta micropropagation through axillary budsAbstract -The objective of this work was to evaluate the use of growth regulators, antioxidant additives, type of explants, and coverage intensity of acclimatized seedlings in the micropropagation of sucupira-preta (Bowdichia virgilioides) through axillary buds. Explants were cultured for multiplication on WPM medium, supplemented with concentrations of benzylaminopurine (BAP). In the elongation phase, treatments consisted of naphthaleneacetic acid (NAA) and BAP combinations added to the medium. For rooting, shoots were inoculated in medium containing concentrations of indolbutyric acid or combinations of the additives polyvinylpyrrolidone and charcoal, in different NAA concentrations. Plants were transplanted to plastic cups with substrate, and covered with polyethylene bags. Later, the bags were withdrawn, or perforated, or not withdrawn, which constituted the in vitro pre-acclimatization treatments. Ex vitro acclimatization was carried out after the period of in vitro pre-acclimatization. Sucupira-preta had the best micropropagation using: cotyledon segments and 0.3 mg L -1 BAP, for multiplication; 0.3 mg L -1 NAA and 0.03 mg L -1 BAP, for elongation; and 2,0 mg L -1 activated carbon for rooting and for the in vitro pre-acclimatization, with the use of a transparent plastic cover around seedling.Index terms: Bowdichia virgilioides, tissue culture, native species, propagation, growth regulators. IntroduçãoA sucupira-preta (Bowdichia virgilioides Kunth.) é uma espécie ...

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Micropropagação de violeta-africana (Saintpaulia ionantha Wendl.): efeito da Benzilaminopurina na multiplicação

Cited by 5 publications

References 4 publications

Multiplicação in vitro de Codonanthe devosiana

Multiplicação in vitro de Codonanthe devosiana

Concentrações de BAP sobre a proliferação in vitro de brotos de Lippia alba [(Mill.)N.E.Brown]

Micropropagação de sucupira-preta por meio de gemas axilares

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