Resumo -O objetivo deste trabalho foi estimar os parâmetros genéticos e predizer o valor genético de populações e indivíduos oriundos de populações segregantes de trigo, com o uso da metodologia de modelos mistos ("restricted maximum likelihood"/"best linear unbiased prediction", REML/BLUP). Trinta e seis populações segregantes de trigo e quatro controles foram avaliados na geração F 3 , em delineamento de blocos ao acaso, com informações de indivíduo retiradas de dentro das parcelas. Os caracteres avaliados foram: produção de grãos, índice de colheita, número de perfilhos e altura de planta. Observou-se a existência de variabilidade genética entre populações em todos os caracteres avaliados. A herdabilidade média variou de 39,15 a 92,78%, e a acurácia, de 62,57 a 96,32%, na seleção de populações. A herdabilidade individual no sentido restrito foi baixa dentro das populações, em todos os caracteres. A acurácia na seleção individual apresentou magnitude média, quanto ao caráter altura de plantas, e baixa quanto aos demais caracteres. A herdabilidade individual contribui para maior ganho nos caracteres altura de planta e índice de colheita com o uso do BLUP individual, em comparação ao BLUP de populações. As populações segregantes Embrapa22/BRS207, Embrapa22/ VI98053, Embrapa22/IVI01041, BRS254/BRS207, BRS254/VI98053, BRS254/UFVT1Pioneiro e BRS264/ BRS207 destacam-se por apresentar valor genético aditivo elevado em dois ou mais caracteres.Termos para indexação: Triticum aestivum, análise de deviance, dados desbalanceados, estratégias de melhoramento, população segregante, REML/BLUP. Estimation of genetic parameters and prediction of additive genetic value for wheat by mixed modelsAbstract -The objective of this work was to estimate the genetic parameters and to predict the genotypic value of populations and individuals from wheat segregating populations, using the methodology of mixed models (restricted maximum likelihood/best linear unbiased prediction, REML/BLUP). Thirty-six wheat segregating populations and four controls were evaluated in the F 3 generation, in a randomized complete block design, with individual information taken from within the plots. The evaluated traits were: grain yield, harvest index, number of tillers, and plant height. Genetic variability between populations was observed for all evaluated traits. The mean heritability varied from 39.15 to 92.78%, and accuracy varied from 62.57 to 96.32% in the selection of populations. The narrow-sense individual heritability was low within populations for all traits. The accuracy for individual selection had a moderate value for plant height, and low values for the other traits. Individual heritability contributes to a greater gain for the traits plant height and harvest index with the use of individual BLUP, in comparison to population BLUP. The segregating populations Embrapa22/BRS207, Embrapa22/VI98053, Embrapa22/IVI01041, BRS254/BRS207, BRS254/VI98053, BRS254/UFVT1Pioneiro, and BRS264/BRS207 stand out with high additive genetic value, for two or mor...
ABSTRACT. We aimed to evaluate 40 common bean cultivars recommended by various Brazilian research institutions between 1970 and 2013 and estimate the genetic progress obtained for grain yield and other agronomic traits. Additionally, we proposed a bi-segmented nonlinear regression model to infer the year in which breeding began to show significant gains in Brazil. The experiment was carried out in Viçosa/MG and Coimbra/MG, in the dry and winter seasons of 2013. For this, a randomized complete block design with three replications was employed. The following traits were evaluated: number of pods per plant (NPP); number of seeds per pod (NSP); 1000-seed weight (W1000); grain yield (Yield); plant architecture (Arch); and grain aspect (GA). Genotypic means were estimated over years using linear mixed models, and genetic gains were estimated using bi-segmented nonlinear regression models. In summary, the methodology proposed in the present study indicated that bean breeding programs in Brazil began to influence Yield beginning in 1990, resulting in a gain of 6.74% per year (68.15 kg/ha per year). The years from which estimated genetic progress for NPP (5.62% per year), NSP (4.59% per year), W1000 (2.08% per year), and GA (1.36% per year) began to increase were 1994, 1990, 1989, and 1986, respectively.
The quality and quantity of light are important factors in controlling in vitro plant growth in photoautotrophic systems. The aim of this study was to evaluate the influence of light quality (fluorescent, white, red, blue, red/blue, and distant red) on microstumps of a Eucalyptus urophylla clone in an in vitro photoautotrophic system, as well as the intensity of fluorescent light (60, 85, 100, and 140 μmol m–2 s–1) in the growth and production of microcutting. The number of shoots and microcutting, the size of the largest shoot, the stomatal density, chlorophyll, and carotenoid content were analyzed. Light quality altered plant growth, and fluorescent light intensity did not affect the microstumps’ production during the evaluation period. In white light-emitting diode (LED) light, there was higher production of carotenoids, with a lower initial production of microcuttings. A smaller number of shoots were obtained in blue LED. In general, the different qualities and light intensities tested allowed for the growth of the Eucalyptus urophylla clone grown in vitro, making it possible to obtain microcuttings under photoautotrophic cultivation. Study Implications In vitro propagation is a stressful process for plants and has limitations for commercial-scale Eucalyptus production. Fluorescent lamps, closed containers, and high sucrose concentrations are traditionally used. To reduce costs and improve production, the use of efficient light sources and photoautotrophic cultivation systems become alternatives. This study investigated the influence of light on the in vitro growth of a Eucalyptus clone in a photoautotrophic system. The quality was more important than the intensity of light. Foresters will be able to indicate the use of LEDs (light-emitting diodes) as a replacement for fluorescent lamps. This approach is useful in enhancing micropropagation techniques.
ResumoOs métodos utilizados para seleção de cruzamentos em uma cultura não necessariamente são apropriados para outras. O presente trabalho teve como objetivo avaliar a aplicação da análise dialélica, da diversidade genética, da média parental e do método de Jinks e Pooni na seleção de genitores e populações segregantes superiores para o caráter rendimento de grãos em trigo. Para isso, foram conduzidos dois experimentos. Os tratamentos foram dispostos em látice (experimento I) e em blocos casualizados com informação de indivíduo dentro da parcela (experimento II). Avaliaram-se 12 genitores e as 36 populações segregantes na geração F 3 resultantes do cruzamento desses genitores em esquema de dialelo parcial. A aplicação das metodologias avaliadas produziu resultados distintos. A média parental e a diversidade genética isoladamente não constituem método de predição eficiente. A aplicação da metodologia de Jinks e Pooni apresenta limitação de ordem prática, não se adequando ao sistema de cultivo do trigo. Dentre as metodologias avaliadas, a análise dialélica constitui o método mais promissor para identificação de genitores e populações segregantes superiores.Palavras-chave: Triticum aestivum, predição do potencial genético, capacidade de combinação, rendimento de grãos. Comparison of methods for selection of parents and segregating populations applied to wheat breeding AbstractThe methods used for selecting crosses in one crop species are not necessarily appropriate for others. The present study aimed to evaluate the application of diallel analysis, genetic diversity, parental average, and the Jinks and Pooni method for the selection of parents and superior segregating populations for the grain yield character in wheat. Thus, two experiments were conducted and the treatments were arranged in lattice (experiment I) and randomized blocks with individual information within the plot (experiment II). Twelve parental individuals and 36 segregating populations in the F 3 generation resulting from the crossing of these parents in a partial diallel arrangement were evaluated. The application of the evaluated methodologies led to distinct results. The parental average and genetic diversity alone are not efficient prediction methods. The methodology of Jinks and Pooni presents practical limitation because it is not adequate for wheat crop system. Among the evaluated methodologies, diallel analysis is the most promising method for the identification of parents and superior segregating populations.
The proposed models were adequate for estimating lettuce pigments in a quick and non-destructive way, representing an alternative to conventional measurement methods. Prediction accuracies were improved by using the detrending, smoothing and first derivative pretreatments to the original spectral signatures prior to estimating lettuce chlorophyll, carotenoid and anthocyanin content, respectively. © 2016 Society of Chemical Industry.
The objective of the present study was to test the effects of explant type, auxin concentrations, culture media, and auxin concentrations on the induction and proliferation of somatic embryos of Eucalyptus grandis x E. urophylla. Seeds and cotyledons were used as explants and inoculated in culture media containing 1.13, 2.26, 3.39 and 4.52 µM dicamba or 4.14, 10.35, 20.71 and 31.06 µM picloram. Embryogenic calli induced in the picloram treatments were used as explants and inoculated in semisolid or liquid media containing 4.14, 10.35, 20.71 and 31.06 µM picloram and keeping the origin of the embryogenic callus (seeds or cotyledons) and the concentration of picloram in those who were in the induction phase. Statistical, descriptive and anatomical analyses were performed. Induction of somatic pro-embryos into the juvenile plant material of Eucalyptus grandis x E. urophylla was performed using seeds or cotyledons as the source of explants, with the addition of dicamba and picloram as growth regulators. The use of cotyledons as a source of explants and the concentration of 4.1 µM picloram added to the culture media resulted in a higher induction of somatic pro-embryos. Proliferation of secondary somatic embryos was achieved using liquid medium added with picloram.
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