Micronucleus assay in vivo provides significant prognostic information in human cervical carcinoma; the updated analysis

Wideł, Maria; Kołosza, Zofia; Jedrus, S.; Lukaszczyk, Beata; Raczek-Zwierzycka, Katarzyna; Świerniak, Andrzej

doi:10.1080/09553000110035558

Cited by 22 publications

(7 citation statements)

References 4 publications

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“…In the cervix tumor cells MN have good predictive value after one week of therapy -high number of MN compared to the background level predict good response to radiotherapy [29]. The same results were obtained by the research group of Wiedel -they investigated the tumor cells obtained with biopsy instead of exfoliated tumor cells [3,4].…”

Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levesupporting

confidence: 57%

“…It is well established that radio and chemother apy widely used for treatment of cancer patients induce chromosomal aberrations and micronuclei (MN) both in tumor [1][2][3][4] and normal (healthy) cells [5,6]. Because of some technical difficulties (invasive procedure to obtain tumor cells during and after treatment) cytogenetic disturbances are mostly studied in lymphocytes [7,8].…”

Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levementioning

confidence: 99%

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Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

Nersesyan

2007

Cytol. Genet.

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Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levesupporting

confidence: 57%

Section: The Data Concerning Possibility To Use Micronuclei (Mn) Levementioning

confidence: 99%

Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

Nersesyan

2007

Cytol. Genet.

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“…There was a good number of cervical cells with both chromosome loss (centromere positive micronucleus) and breakage (centromere-lacking micronucleus) in the LSIL and HSIL categories. In fact, many researchers have observed the higher frequencies of micronucleated cells among cancer patients compared to healthy individuals [29,30]. The ease and low cost of the detection of MN may allow it as a prognostic indicator during the planning and validation of programs for cancer monitoring and prevention.…”

Section: Discussionmentioning

confidence: 99%

Micronucleus in Cervical Intraepithelial Lesions and Carcinoma

Samanta¹,

Dey

Nijhawan

2010

Acta Cytologica

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Aims and Objectives: To score and compare micronucleus (MN) in the whole spectrum of cervical lesions including normal, inflammatory, abnormal squamous cell of undetermined significance (ASC-US), low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL) and invasive cancer (IC) and to evaluate the role of MN as a biomarker in different pre-neoplastic and neoplastic lesions. Materials and Methods: A total of 224 slides, comprised of normal (40), inflammatory (40), ASC-US (30), LSIL (38), HSIL (30) and IC (46), were studied. All the cases of HSIL, IC and ASC-US had histopathology. The LSIL, normal and inflammatory smears were again reviewed by 2 experienced cytopathologists independently. Two observers separately and independently counted the number of micronucleated cells per 1,000 of epithelial cells in oil immersion magnification (×100 objective) which was expressed as MN score per 1,000 cells. Results: The mean MN scores ± SD in normal, inflammatory, ASC-US, LSIL, HSIL and IC cases of cervical lesions were 1.02 ± 1.59, 0.4250 ± 0.71208, 2.87 ± 2.21, 4.7368 ± 5.62179, 21.30 ± 17.18 and 18.50 ± 9.54, respectively. MN scores of IC and HSIL were significantly high compared to the normal (p < 0.000), the inflammatory (p < 0.000), the ASC-US (p < 0.000) and to the LSIL (p < 0.000) group (analysis of variance test). LSIL showed significant difference with the normal (p = 0.043), the inflammatory (p = 0.019), the HSIL (p < 0.000) and the IC (p < 0.000) group but not with the ASC-US (p = 0.342) group. Conclusions: MN scoring on the epithelial cells of cervix could be used as a biomarker in cancer screening. This is an easy, simple, reliable, reproducible and objective test which can be performed on routinely stained smears.

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“…The MN frequency in control (non-irradiated) cells, representing so-called spontaneous MN was regarded as a baseline level. The percentage of binucleated cells (%BNC) in non-irradiated cells was used for measurement of proliferation fraction in vitro (Widel et al, 2001). …”

Section: Microscopic Evaluation and Mn Scoringmentioning

confidence: 99%

“…Micronuclei can be measured after DNA staining by the Feulgen reaction, Giemsa or fluorescence dyes. Evaluation of micronucleus frequency is widely used for determination of cellular radiosensitivity (Wandl et al, 1989;Shibamoto et al, 1991, Widel et al, 2001. Generally, radiosensitive cells are more susceptible to radiation-induced micronucleus formation.…”

Section: Introductionmentioning

confidence: 99%

Cytokinesis-Block Micronucleus Assay in Human Glioma Cells Exposed to Radiation

Słowiński¹,

Bierzyńska-Macyszyn²,

Mazurek³

et al. 2011

Image Anal Stereol

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Biological tests are efficient in reflecting the biological influences of several types of generally harmful exposures. The micronucleus assay is widely used in genotoxicity studies or studies on genomic damage in general. We present methodological aspects of cytokinesis-block micronucleus assay performed in human gliomas irradiated in vitro. Eight human glioblastoma cell lines obtained from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Germany) were gamma-irradiated ( 60 Co) over a dose range of 0-10 Gy. Cytokinesis-block micronucleus assay was performed to quantitate cytogenetic damage. The cells were fixed directly on dishes, stained with fluorochrome DAPI and evaluated under fluorescent and phase contrast microscope. The micronucleus frequency was expressed as a micronuclei (MN) per binucleated cell (BNC) ratio, calculated after scoring at least 100 BNC per dish. The frequency of spontaneous MN ranged from 0.17 to 0.613 (mean: 0.29 ± 0.14). After irradiation increase of MN frequency in the range of 0.312 -2.241 (mean: 0.98 ± 0.68) was found at 10 Gy. Gliomas are extremely heterogenous in regard to cytogenetic effects of irradiation, as shown in this study by cytokinesis-block micronucleus assay. This test is easily performed on irradiated glioma cell lines and can assist in determining their radiosensitivity. However, in order to obtain reliable and reproducible results, precise criteria for MN scoring must be strictly followed. Simultaneous use of fluorescent and phase contrast equipment improves imaging of morphological details and can further optimize MN scoring.

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Micronucleus assay in vivo provides significant prognostic information in human cervical carcinoma; the updated analysis

Cited by 22 publications

References 4 publications

Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

Micronucleus in Cervical Intraepithelial Lesions and Carcinoma

Cytokinesis-Block Micronucleus Assay in Human Glioma Cells Exposed to Radiation

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