Cytokinesis-Block Micronucleus Assay in Human Glioma Cells Exposed to Radiation

Słowiński, Jerzy; Bierzyńska-Macyszyn, G; Mazurek, Urszula; Wideł, Maria; Latocha, Małgorzata; Stomal, Monika; Śnietura, Mirosław; Mrówka, R

doi:10.5566/ias.v23.p159-165

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…Micronucleus tests describe micronuclei of all chromosomes or lagged chromosome fragments caused by cytogenic damage. MN assay with cytokinesis‐block induced by cytochalasin B was performed according to the method described by Yared et al as modified by Slowinski et al Following disaggregation of routinely MCF‐7 cells with trypsin/EDTA and resuspension in complete medium, 3 mL aliquots (10 4 cells) were seeded into 30‐mm petri dishes containing 20‐mm coverslips. The cells were then allowed to attach for overnight prior to HMS treatment.…”

Section: Methodsmentioning

confidence: 99%

Assessment of the cytotoxicity and genotoxicity of homosalate in MCF‐7

Yazar

Ertekin

2019

J of Cosmetic Dermatology

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Sunscreens (UV filters) can be found in cosmetics and in other personal care products, but also in food packaging materials, pharmaceuticals, plastics, textiles, and vehicle maintenance products to prevent the photodegradation of polymers and pigments. Organic UV filters are often used together with inorganic filters to increase the overall efficacy. 1,2 HMS (3,3,5-trimethylcyclohexylsalicylate; Figure 1) is an organic UV filter that absorbs UVB radiation at wavelengths from 290 to 320 nm, that can be found in many cosmetics. 3 The presence of alkyl-substituted cyclohexane ring in UV filters can considerably alter the lipophilicity of the whole molecule which might possibly have a significant effect on metabolism. 4 Studies have shown that HMS passes by systemic circulation after topical application of a gel formulation in rats. 3 Hence, HMS has been shown to cause hormonal dysfunction when absorbed in in vivo and in vitro studies. 5-7 HMS accumulates in aquatic biota and humans through the food chain 1,8 ; therefore, it is important to consider the effects on health and know its mechanisms of action.

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Section: Methodsmentioning

confidence: 99%

Assessment of the cytotoxicity and genotoxicity of homosalate in MCF‐7

Yazar

Ertekin

2019

J of Cosmetic Dermatology

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“…The CBMN test was performed as reported elsewhere with minor modifications [ 49 ]. After 16 h of culturing CHO-K1, three experimental conditions were performed.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador

Bailón-Moscoso

Tinitana

Martínez-Espinosa

et al. 2017

BMC Complement Altern Med

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Background“Horchata” is an herbal mixture infusion consumed in Southern Ecuador; 66% of its plants are anti-inflammatory medicinal plant, and 51% are analgesics. Anti-inflammatory substances can prevent carcinogenesis mediated by cytotoxic effects and can prevent DNA damage. The aim of this study was to evaluate the cytotoxicity and apoptotic/antigenotoxic effects of horchata as well as its mechanism.MethodsNine different varieties of horchata were prepared in the traditional way and then freeze-dried. Phytochemical screening tested for the presence of secondary metabolites using standard procedures and antioxidant activities. The cytotoxic activity was evaluated on cerebral astrocytoma (D-384), prostate cancer (PC-3), breast cancer (MCF-7), colon cancer (RKO), lung cancer (A-549), immortalized Chinese hamster ovary cells (CHO-K1), and human peripheral blood lymphocytes via a MTS assay. The pro-apoptotic effects were evaluated with Anexin V/Propidium Iodide and western blot of Bax, Bcl-2, TP53, and TP73. Induction and reduction of ROS were assessed by fluorimetry. Genotoxic and antigenotoxic effects were evaluated with a comet assay and micronuclei on binucleated cells.ResultsFive of nine horchatas had cytotoxic effects against D-384 while not affecting normal cells. These horchatas induce cell death by apoptosis modulated by p53/p73. In CHO-K1 cells, the horchatas decrease the damage induced by hydrogen peroxide and Mitomycin C measured in the comet and micronucleus assay respectively.ConclusionsThe IC50 range of effective horchatas in D-384 was 41 to 122 μg·mL−1. This effect may be related to its use in traditional medicine (brain tonic). On the other hand, immortalized Chinese hamster ovary cells (CHO-K1) and lymphocytes did not show a cytotoxic effect. The most potent horchata induced apoptosis via a p53/p73-mediated mechanism.The horchatas present antigenotoxic properties, which may be related to the antioxidant capacity. Future studies on horchata components are necessary to understand the interactions and beneficial properties.

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“…These structures are excluded from the daughter nuclei into the cytoplasm. 4 Micronucleus is the target structure for the toxicological screening to explore potential genotoxicity of a material. The in vitro chromosomal aberration potential of a material could be done using cytokinesis-block assay.…”

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confidence: 99%

Bisphenol-A Release and Genotoxicity Differences of Three Branded Adhesives Materials

Winarta¹,

Amtha²,

Roeslan³

et al. 2015

J Dent Indones

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Objectives: The objective of the study were to determine if there was any (bisphenol A) BPA release from three adhesive brands, to determine the differences of BPA release between three adhesive brands, to determine the genotoxicity from three adhesive brands, and to determine the correlation of BPA release and genotoxicity. Methods: Three branded adhesives materials were polimerized in mold and immersed in pH 7 and 4 artificial saliva from 24 to 720 hours. The artificial saliva was tested with spectrophotometry test to see BPA release at 24, 240, 480, and 720 hours, then freeze dried to get solid extract. Combination of the extract and lymphocite culture (male and female) then tested with in vitro cytokinesis-block micronucleus (MN) assay to see genotoxicity level of three adhesives at 24, 240, 480, and 720 hours as well. Results: The BPA release occured at 720 hours by Adhesive 1: 0.013μg/L; Adhesive 2: 0.11μg/L; Adhesive 3: 0.036μg/L. There was a statistically significant difference between BPA release with time (F = 505.98; p=0.00) and brands (F = 147.65; p = 0.00). Time and BPA release interaction also showed a statistically significant difference (F=13.35; p=0.00). Genotoxicity can be seen at 720 hours on Flowtain LV sample (MN frequency: male: 0.044; female: 0.053). Conclusion: The number of BPA release of all brand can be seen from the first 24 hours, and were increasing from 24 to 720 hours. Genotoxicity can be seen from one of the adhesive brand at 720 hours.There was correlation between BPA leaching and micronucleus frequency.

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Cytokinesis-Block Micronucleus Assay in Human Glioma Cells Exposed to Radiation

Cited by 5 publications

References 24 publications

Assessment of the cytotoxicity and genotoxicity of homosalate in MCF‐7

Assessment of the cytotoxicity and genotoxicity of homosalate in MCF‐7

Cytotoxic, antioxidative, genotoxic and antigenotoxic effects of Horchata, beverage of South Ecuador

Bisphenol-A Release and Genotoxicity Differences of Three Branded Adhesives Materials

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