Microdetermination of Proteins by Resonance Light Scattering Spectroscopy with Bromophenol Blue

Qi, Chun; Li, Ke An; Shen, Tong

doi:10.1006/abio.1996.0294

Cited by 73 publications

(29 citation statements)

References 11 publications

(14 reference statements)

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“…[9][10][11] RLS is a unique scattering with an identical frequency for both the electromagnetic wave absorbed by electrons and that of the scattering, and determinations are usually performed based on the substantial enhancement of RLS intensity in the presence of nucleic acids. A number of dyes have been employed for RLS determination of nucleic acids; [12][13][14][15] however, ethidium bromide has not been applied for this purpose so far.…”

Section: Introductionmentioning

confidence: 99%

An Abnormal Resonance Light Scattering Arising from Ionic‐Liquid/DNA/Ethidium Interactions

Cheng

Chen

Wang

et al. 2007

Chemistry A European J

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In the aqueous phase, ethidium bromide (EB) intercalates into the double helix structure of dsDNA (ds=double-stranded) with a notable enhancement in fluorescence and resonance light scattering (RLS). However, when dsDNA was extracted into an ionic liquid (IL), 1-butyl-3-methylimidazolium hexafluorophosphate (BmimPF(6)), an abnormal RLS arising from the interactions of IL-DNA-EB was observed, with a substantial decrease of the recorded RLS. The cationic Bmim(+) groups of BmimPF(6) intercalate into the DNA helix structure, in which they interact with the P-O bonds of phosphate groups in DNA strands and result in a reduction of the base-pair interstice along with transformation of DNA conformations that consequently prohibits the intercalation of EB with DNA. Thus, in the IL phase, the interactions between ethidium and DNA were dominated by electrostatic interactions and hydrogen bonding, leading to a congregation of EB entities around the DNA strands that results in an increase of absorption by ethidium, and consequently the inner filter effect leads to a reduction of the RLS. The present observation has been applied to the direct quantification of DNA in an ionic-liquid phase after DNA from human whole blood was extracted into BmimPF(6).

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Section: Introductionmentioning

confidence: 99%

An Abnormal Resonance Light Scattering Arising from Ionic‐Liquid/DNA/Ethidium Interactions

Cheng

Chen

Wang

et al. 2007

Chemistry A European J

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“…Resonance Rayleigh scattering (RRS), as a sensitive and simple detection technique, has been applied to determine macromolecules such as nucleic acids (26), proteins (27), heparin (28), metal ions (29), non-metal ions (30) and organic compounds (31). Recently, RRS has been increasingly used in drug analysis (32)(33)(34).…”

Section: Introductionmentioning

confidence: 99%

Study on the interaction between palladium(II)–lincomycin chelate and erythosine by absorption, fluorescence and resonance Rayleigh scattering spectra and its analytical applications

Liu

et al. 2008

Luminescence

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In pH 5.0-5.4 HAc-NaAc buffer solution, lincomycin (Linco) reacted with Pd(II) to form 1:1 cationic chelate, which could further react with erythrosine (Ery) to form 1:1 ion-association complexes (Pd-Linco)Ery. As a result, not only were the absorption and fluorescence spectra changed, but also the resonance Rayleigh scattering (RRS) intensity was greatly enhanced. These phenomena offered useful means for the determination of Linco by spectrophotometry, fluorescence and RRS methods. The linear range and detection limit of Linco were 0.20-3.00 mg/mL and 0.057 mg/mL, 0.20-4.80 mg/mL and 0.061 mg/mL, 0.05-2.70 mg/mL and 0.015 mg/mL for the spectrophotometric, fluorescence quenching and RRS methods, respectively. Among these, the RRS method obtained the highest sensitivity. Therefore, the optimum reaction conditions and the influences of coexisting substances were investigated using the RRS method. A simple, sensitive and rapid method has been developed for the determination of Linco in either the pharmaceutical form or human body fluids, and the reasons for RRS enhancement are discussed.

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“…Ma et al (9)(10)(11) used this technique for protein assay, and subsequently this method has attracted the attention of many researchers (12)(13)(14)(15).…”

mentioning

confidence: 98%

Resonance light scattering technique for the determination of proteins with Congo red and Triton X‐100

Sun

Guo

et al. 2005

Luminescence

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Resonance light scattering (RLS) of Congo red (CR) was greatly enhanced by BSA (HSA) in the presence of Triton X-100 (TX-100). In sodium citrate-HCl buffer (pH 2.7-3.0), the enhanced intensity of resonance light scattering at 360 nm was in proportion to the concentration of proteins [corrected] The linear relationship was obtained between the resonance light scattering intensity and proteins in the range 5.0 x 10(-8)-8.0 x 10(-6) g/mL and 1.0 x 10(-9)-6.0 x 10(-6) g/mL for BSA and HSA, respectively. Their detection limits were 1.4 x 10(-8) g/mL and 2.8 x 10(-10) g/mL (S:N = 3), respectively. Synthetic and actual samples were analysed satisfactorily.

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Microdetermination of Proteins by Resonance Light Scattering Spectroscopy with Bromophenol Blue

Cited by 73 publications

References 11 publications

An Abnormal Resonance Light Scattering Arising from Ionic‐Liquid/DNA/Ethidium Interactions

An Abnormal Resonance Light Scattering Arising from Ionic‐Liquid/DNA/Ethidium Interactions

Study on the interaction between palladium(II)–lincomycin chelate and erythosine by absorption, fluorescence and resonance Rayleigh scattering spectra and its analytical applications

Resonance light scattering technique for the determination of proteins with Congo red and Triton X‐100

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