“…Subsequently, Huang et al first used this technique for analytical purposes (16). Now the RLS technique followed spectrophotometric method and fluorescence method has become very attractive for the determination of biological molecules, such as nucleic acids (17)(18)(19), proteins (20)(21)(22)(23)(24) and amino acids (25). A series of reagents, such as α,β,γ,δ -tetrakis [4-(trimethylammoniumyl)phenyl] porphyrin (TAPP) (16), dequalinium chloride (26), morin (27), Azur B (28), hexaamine cobalt (III) ion (29), Y(III)-1,6-bi(1′-phenyl-3′-methyl-5′-pyrazolone-4′-)hexanedione(BPMPHD) (30), have been successfully exploited to determine nucleic acids.…”