Resonance light scattering technique for the determination of proteins with Congo red and Triton X‐100

Abstract

Resonance light scattering (RLS) of Congo red (CR) was greatly enhanced by BSA (HSA) in the presence of Triton X-100 (TX-100). In sodium citrate-HCl buffer (pH 2.7-3.0), the enhanced intensity of resonance light scattering at 360 nm was in proportion to the concentration of proteins [corrected] The linear relationship was obtained between the resonance light scattering intensity and proteins in the range 5.0 x 10(-8)-8.0 x 10(-6) g/mL and 1.0 x 10(-9)-6.0 x 10(-6) g/mL for BSA and HSA, respectively. Their detection limits were 1.4 x 10(-8) g/mL and 2.8 x 10(-10) g/mL (S:N = 3), respectively. Synthetic and actual samples were analysed satisfactorily.

Study on interactions of aminoglycoside antibiotics with calf thymus DNA and determination of calf thymus DNA via the resonance Rayleigh scattering technique

“…Weak binding forces between molecules, such as Van der Waal's force, electrostatic attraction, hydrogen bonding, hydrophobic interaction and the aggregation of biological macromolecules, can easily cause the changes in RRS spectroscopy . Moreover, because RRS assays have many other advantages such as low sample volume, simple operation, high sensitivity and selectivity, this method has been widely applied to macromolecule detection and the study of interactions between DNA and other substances such as dyes , drugs , proteins , metal ions and surfactants . In addition, recent progress in the analysis of nucleic acids using the RRS method has increased the reliability and maturity of this approach.…”

Resonance light scattering study on the interaction between quinidine sulfate and congo red and its analytical application

“…Congo red, a kind of bisazo anionic dyes, is a sensitive RLS probe and has been used to determine many chemicals, such as proteins, [27] nucleic acids [28] and drugs. [29][30][31] In the experiments of this paper, the interaction between QDS and CR to form a supermolecular complex was studied using RLS.…”

Development of a sensitive and rapid nucleic acid assay with tetraphenyl porphyrinatoiron chloride by a resonance light scattering technique

“…Subsequently, Huang et al first used this technique for analytical purposes (16). Now the RLS technique followed spectrophotometric method and fluorescence method has become very attractive for the determination of biological molecules, such as nucleic acids (17)(18)(19), proteins (20)(21)(22)(23)(24) and amino acids (25). A series of reagents, such as α,β,γ,δ -tetrakis [4-(trimethylammoniumyl)phenyl] porphyrin (TAPP) (16), dequalinium chloride (26), morin (27), Azur B (28), hexaamine cobalt (III) ion (29), Y(III)-1,6-bi(1′-phenyl-3′-methyl-5′-pyrazolone-4′-)hexanedione(BPMPHD) (30), have been successfully exploited to determine nucleic acids.…”