Rayleigh light scattering of Acid Chrome Blue K (ACBK) is enhanced greatly by proteins. Based on this, a method for protein assay in aqueous solution was developed. This assay matches the sensitivity of the colorimetric dye-binding method with a linear range of 0.136-10.88 micrograms ml-1. The measurements can be made easily on a common fluorimeter. The reaction between ACBK and proteins is completed in 2 min and the scattered light signal is stable for at lest 3 h. Protein-to-protein variability is encountered in this method as in many other protein assays. There is little or no interference from amino acids, most metal ions and complexing agents (e.g., EDTA). Interferences from salts, urea and detergents can be minimized by dilution.
A method for the quantitation of proteins in aqueous solution involving the binding of bromopyrogallol red to proteins under acidic conditions has been developed. The binding of the dye to proteins is accompanied by an enhancement of Rayleigh light scattering at 332 nm; the scattering intensity is linear over the range 0.136—6.80 μg ml−1. The reaction is completed immediately after mixing dye and protein solutions, and the scattering signal is stable for at least 3 h. There are very few interference with the method, most of which can be minimized by dilution.
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