Enhancement of Rayleigh Light Scattering of Acid Chrome Blue K by Proteins and Protein Assay by the Scattering Technique

Abstract

Rayleigh light scattering of Acid Chrome Blue K (ACBK) is enhanced greatly by proteins. Based on this, a method for protein assay in aqueous solution was developed. This assay matches the sensitivity of the colorimetric dye-binding method with a linear range of 0.136-10.88 micrograms ml-1. The measurements can be made easily on a common fluorimeter. The reaction between ACBK and proteins is completed in 2 min and the scattered light signal is stable for at lest 3 h. Protein-to-protein variability is encountered in this method as in many other protein assays. There is little or no interference from amino acids, most metal ions and complexing agents (e.g., EDTA). Interferences from salts, urea and detergents can be minimized by dilution.

Resonance light‐scattering enhancement effect of the protein–Y3+–TTA–SLS system and its analytical application

“…Since Pasternack developed a resonance light scattering (RLS) technique with a common spectroflurimeter (12,13), this technique has been widely applied to determine nucleic acids (14)(15)(16) and proteins (17)(18)(19)(20). The RLS technique is based on the following phenomenon: when the wavelength of the incident beam is close to the absorption band of molecular particles which exist as aggregates, the Rayleigh scattering intensity of some wavelengths will be much higher than normal light scattering (21).…”

Resonance light scattering technique for the determination of proteins with Congo red and Triton X‐100

“…Ma et al (9)(10)(11) used this technique for protein assay, and subsequently this method has attracted the attention of many researchers (12)(13)(14)(15).…”

Rayleigh Light Scattering Study on the Reaction of Nucleic Acids and Methyl Violet

“…This technique is an important tool for biomolecule analysis, although the enhancement of resonance light scattering requires the aggregation of dyes on nucleic acids. Ma et al (14,15) and Wei et al (16) found that some small molecules that do not selfaggregate can also produce enhanced light scattering signals when they react with proteins. This type of scattering is not resonance light scattering, but Rayleigh light scattering (RLS) 2 (14).…”