Resonance light‐scattering enhancement effect of the protein–Y³⁺–TTA–SLS system and its analytical application

Abstract

In this paper, a sensitive resonance light scattering (RLS) method for the determination of protein is reported. In the Tris-HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y(3+)-2-thenoyltrifluoroacetone (TTA)-sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 x 10(-9)-1.0 x 10(-5) g/mL for BSA, 1.0 x 10(-8)-1.0 x 10(-5) g/mL for HSA and 1.0 x 10(-8)-1.0 x 10(-6 )g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied.

SPR‐enhanced fluorescence and protein‐improved blood compatibility of quadruple core/shell nanostructure of Ag@SiO2@Eu3+(tta)3Phen@Protein

“…First, in the structure of the Eu 3+ (tta) 3 Phen, the TTA and Phen can form the first shell to cover the surface of the Eu 3+ , which make the conjugated group of the TTA and Phen have the org–org interaction with the amide bond of lysozyme [30, 31]. At the same time, the polar nitrogen and fluorine atom of the ligands can also produce the polar–polar interaction with the lysozyme [32, 33].…”

Study on the Interaction between a Novel Ionic Liquid Probe and Anionic Surfactants Using Resonance Light Scattering Spectra and Its Analytical Application

“…In recent years, resonance light scattering (RLS), as a highly sensitive and simple analytical technique, has wide applications, for example, analysis of protein, 12,13 and nucleic acid, [14][15][16] determination of inorganic ions, 17 organic compounds 18 and pharmaceuticals. 19,20 In addition, RLS technique has also been applied to determine AS in aqueous environment with various RLS probe reagents, such as acridine orange, 21 {Co(4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene) 2 } 2 ＋ , 22 chlorpromazine hydrochloride, 23 and janus green.…”

Resonance light scattering study on the interaction between quinidine sulfate and congo red and its analytical application

“…[9] Analytical procedures applying the RLS method combine the advantages of simplicity, sensitivity and rapidity. [10][11][12][13][14] Since Huang et al first used this technique for analytical purposes, [10] much attention has been paid to the study and determination of nucleic acids, [15][16][17] proteins [18][19][20] and inorganic ions [21][22][23] by use of this method. In recent years, RLS has been widely applied to determine some pharmaceuticals.…”